Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Bareh, G.M.; Jacoby, Ethan; Binkley, Peter; Chang, T. Arthur; Schenken, Robert S.; Robinson, Randal D.

doi:10.1016/j.fertnstert.2015.10.033

Cited by 68 publications

(47 citation statements)

References 49 publications

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“…Several studies have reported a negative correlation between SDF and fertilization rates, pregnancy, and embryo quality [52][53][54]. Barehet al reported high incidence of SDF in normozoospermic male partners of couples with recurrent pregnancy loss (RPL) [55]. In the current study, we also noticed high levels of DNA damage in idiopathic infertile men, which decreased significantly following antioxidant supplementation with FH PRO for Men.…”

Section: Discussionsupporting

confidence: 70%

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

et al. 2020

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Antioxidants are used in the empirical treatment of infertile men. The aim of this study was to evaluate the effects of antioxidant therapy on conventional semen parameters and advanced sperm function tests in men seeking fertility treatment. A total of 148 infertile men of unknown etiology were divided into idiopathic (n = 119) and unexplained male infertility (UMI; n = 29). All participants were treated with the antioxidant supplement 'FH PRO for Men' for a period of three months. Compared with pretreatment results, there was a significant improvement in conventional semen parameters including sperm concentration, total and progressive motility and normal morphology, and seminal oxidation reduction potential (ORP), and sperm DNA fragmentation (SDF) in idiopathic infertile men. The changes were more prominent in idiopathic infertile men positive for ORP and SDF. UMI patients showed an improvement in progressive motility, ORP, and SDF after antioxidant treatment. Statistical analysis revealed that the efficacy of FH PRO for Men was significant in idiopathic male infertility compared with UMI. Treatment of idiopathic male infertility patients with the FH PRO for Men antioxidant regimen for three months resulted in a significant improvement in conventional semen parameters and sperm function. Therefore, FH PRO for Men offers promise for the medical treatment of idiopathic male infertility.

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Section: Discussionsupporting

confidence: 70%

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

et al. 2020

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“…Determination of DNA fragmentation in spermatozoa may thus be a relevant, complementary sperm parameter to reinforce current sperm analyses (Agarwal, Majzoub, et al, ; Evgeni, Charalabopoulos, & Asimakopoulos, ; Giwercman et al, ; Kim, ; Lewis, ; Malić Vončina et al, ); particularly when ART is used (Bounartzi et al, ; Bungum et al, ; Yilmaz et al, ). Most interesting is the determination of sperm DNA fragmentation in cases of men diagnosed with idiopathic infertility, where semen variables are within “normal” limits (Bareh et al, ; Oleszczuk, Augustinsson, Bayat, Giwercman, & Bungum, ; Santi, Spaggiari, & Simoni, ).…”

Section: Introductionmentioning

confidence: 99%

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

et al. 2019

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Men affected with idiopathic infertility often display basic spermiogramme values similar to fertile individuals, questioning the diagnostic impact of the World Health Organization (WHO) thresholds used. This study explored sperm DNA fragmentation in single ejaculates from 14 fertile donors and 42 patients with idiopathic infertility providing semen for assisted reproductive techniques in a university fertility clinic. Each ejaculate was simultaneously studied for sperm DNA fragmentation by the flow cytometer‐based sperm chromatin structure analysis (SCSA) and the new light‐microscopy‐based sperm chromatin dispersion assay (SCD‐HaloSpermG2®), before and after sperm selection for in vitro fertilisation with a colloid discontinuous gradient. The WHO semen variables did not differ between groups, but DNA fragmentation after SCSA (DFI) or SCD (SDF) was significantly (p < 0.05) higher in patients (DFI: 40.2% ± 3.0 vs. SDF: 40.3% ± 1.4) than in fertile donors (DFI: 17.1% ± 2.1 vs. SDF: 20.9% ± 2.5). Sperm selection led to lower proportions of DNA‐fragmented spermatozoa (DFI: 11.9 ± 1.7 vs. SCD: 10.0 ± 0.9, p < 0.05). The techniques output correlated highly and significantly (r2 = 0.82). DNA fragmentation is confirmed as a relevant variable for scrutinising patients with idiopathic infertility, beyond the evidently insufficient WHO semen analyses. Since both techniques yielded similar results, the reduced necessity of complex equipment when running SCD ought to be considered for a clinical setting.

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“…Bungum et al (2007) described significantly reduced pregnancy and delivery rates among infertile couples with a sperm DNA fragmentation index (DFI) > 30% compared to those with DFI < 30% in intra uterine insemination (IUI) cycles [2]. Despite the acceptable pregnancy rate achieved by IVF-ICSI for couples with high DFI, reduced pregnancy rates [11] [12] and increased risk for pregnancy loss [13] [1,14,15] remain major concerns. There is still controversy as to whether DFI should be investigated routinely or selectively among subgroups of infertile couples [16].…”

Section: Introductionmentioning

confidence: 99%

Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading

et al. 2017

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High DNA fragmentation index (DFI) may be associated with poor outcome after IVF. Our aim was to determine whether DFI impacts blastocyst quality or clinical outcome. This retrospective study included 134 couples who underwent 177 IVF-ICSI and pre-implantation genetic screening (PGS) cycles during January 1st, 2014—March 31st, 2016 and had documented previous DFI. Group 1 (DFI>30%) encompassed 25 couples who underwent 36 cycles; Group 2 (DFI 15–30%) included 45 couples and 57 cycles; group 3 (DFI<15%) included 64 couples and 83 cycles. Male partners within group 1 were older (45.1 compared to 40.6 and 38.3 years, respectively, p<0.05), had higher BMI (32.4 compared to 26.6 and 25.8 respectively, p<0.05) and lower sperm count and motility (46*106/ml and 35.5%, respectively) compared to groups 2 (61.8*106/ml and 46.6%, respectively) and 3 (75.8*106/ml and 55.1%, respectively, p<0.05). Female parameters including ovarian reserve and response and embryo development were similar. Total numbers of biopsied blastocysts were 116, 175 and 259 in groups 1, 2 and 3, respectively. PGS for 24 chromosomes revealed comparable euploidy rate of 46–50.4%, with a similar morphological classification. No significant differences were found regarding pregnancy rates or pregnancy loss. It seems that DFI doesn't correlate with blastocyst aneuploidy or morphological grading.

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Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Abstract: Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

Cited by 68 publications

References 49 publications

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading

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Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Abstract: Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

Cited by 68 publications

References 49 publications

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ® ) comparable?

Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading

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Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?