Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique

Benchaïb, Mehdi; Braun, Valerie; Lornage, Jacqueline; Hadj, S.; Salle, Bruno; Lejeune, Hervé; Guérin, J. F.

doi:10.1093/humrep/deg228

Cited by 411 publications

(328 citation statements)

References 23 publications

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“…Although the different thresholds have applied to assisted reproduction samples, and no IVF or ICSI pregnancies were reported when the DFI of the neat semen sample was >27 % [28]. Similar cut-offs of 28 % for 33 IUI/IVF/ICSI/ cycles [36], 20 % in a group of 104 IVF and ICSI cycles [4] and 27 % for 113 IVF and ICSI cycles have been reported [27]. [10] have also shown the importance of this chromatin assay as an independent predictor in assisted reproduction outcome.…”

Section: Discussionmentioning

confidence: 96%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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Section: Discussionmentioning

confidence: 96%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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“…Other reports, such as Martin and co-authors, demonstrated that oligozoospermic samples have an increased probability of sperm chromosome abnormality, whether from mild to severe oligozoospermia [23], whereas chromosomal abnormalities of spermatozoa are not higher in aging males. It has been shown that low sperm morphology is associated with DNA fragmentation in men with an average age of 35 [24]. In addition, the same investigators reported that an increased DNA fragmentation rate decreases the pregnancy rate.…”

Section: Discussionmentioning

confidence: 97%

“…Young women, of an average age of 24 (range [20][21][22][23][24][25][26], were accepted into the Egg Donation Program (EDP) cycles. All of these women were healthy, had normal menstrual cycles and no determined pathology.…”

Section: Oocytes Donation and Fertilizationmentioning

confidence: 99%

Male age influences oocyte-donor program results

Girsh

Katz

Genkin

et al. 2008

J Assist Reprod Genet

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Background The purpose of our research was to examine the relationship between male age and semen parameters in a range of ages (from 20s to 60s) in Egg Donation Program (EDP) cycles. EDP provides a pool of high quality oocytes, thus allowing better analysis of the sperm efficacy. Discussion The retrospective study population consisted of 484 male partners of patients undergoing EDP in in-vitro fertilization (IVF) treatments. A comparison was made of male age and sperm parameters within two groups: cycles resulting in a pregnancy (pregnant group) and cycles which failed to achieve a pregnancy (non-pregnant group). The men involved in the pregnant group were found to be significantly younger 43.2±8.1 than those of the nonpregnant group 46.8±7.8 (p=0.003). Analysis of sperm morphology revealed a significant prevalence of teratozoospermia in males of the non-pregnant group, as compared to the males of the pregnant group (29% vs. 11%, respectively). The results also demonstrate that sperm parameters are apparently not diminished until men reach the age of 40. However, between 40-50 years old semen parameters deteriorate. Male age was found to be related to a reduction in sperm strict criteria: 44.8 in normozoospermia, 47.9 (p=0.02), 48.4 (p=0.04) and 51.9 (p=0.001) years old in mild teratozoospermia, moderate teratozoospermia and severe teratozoospermia, respectively. Additionally, the results showed that the percentage of "healthy" embryos on day 3 of embryo culture was lower in the non-pregnant group (26%), as compared with the pregnant group (34%; p=0.01). Conclusion Our study confirms that sperm parameters are reduced by age and suggests that this age-dependent effect could be a reason for failures in IVF cycles even in EDP couples.

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“…With ICSI, individual sperm are then selected visually based on motility and morphology by a trained embryologist for injection to an egg 7, 8. However, other important sperm attributes that can affect ART outcomes, including sperm DNA–chromatin or chromosomal integrity, mutational, or methylome composition, and the presence of reactive oxygen species (ROS)9, 10, 11, 12, 13, 14, 15, 16, 17 are typically not considered. In particular, long periods of incubation, as well as shear stresses due to centrifugation, increase ROS accumulation.…”

Section: Introductionmentioning

confidence: 99%

Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

et al. 2017

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Male infertility is a reproductive disease, and existing clinical solutions for this condition often involve long and cumbersome sperm sorting methods, including preprocessing and centrifugation‐based steps. These methods also fall short when sorting for sperm free of reactive oxygen species, DNA damage, and epigenetic aberrations. Although several microfluidic platforms exist, they suffer from structural complexities, i.e., pumps or chemoattractants, setting insurmountable barriers to clinical adoption. Inspired by the natural filter‐like capabilities of the female reproductive tract for sperm selection, a model‐driven design, featuring pillar arrays that efficiently and noninvasively isolate highly motile and morphologically normal sperm, with lower epigenetic global methylation, from raw semen, is presented. The Simple Periodic ARray for Trapping And isolatioN (SPARTAN) created here modulates the directional persistence of sperm, increasing the spatial separation between progressive and nonprogressive motile sperm populations within an unprecedentedly short 10 min assay time. With over 99% motility of sorted sperm, a 5‐fold improvement in morphology, 3‐fold increase in nuclear maturity, and 2–4‐fold enhancement in DNA integrity, SPARTAN offers to standardize sperm selection while eliminating operator‐to‐operator variations, centrifugation, and flow. SPARTAN can also be applied in other areas, including conservation ecology, breeding of farm animals, and design of flagellar microrobots for diagnostics.

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Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique

Abstract: The proportion of sperm with DNA fragmentation appears to be potentially useful as a predictor of ICSI outcome, whereas embryo quality based on morphological criteria, appeared unaffected by DNA fragmentation.

Cited by 411 publications

References 23 publications

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Male age influences oocyte-donor program results

Guidance and Self‐Sorting of Active Swimmers: 3D Periodic Arrays Increase Persistence Length of Human Sperm Selecting for the Fittest

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