Sperm DNA Fragmentation in Saudi Infertile Men with Normal Standard Semen Parameters

Ghedan, Maher Hassan Al; Matrafi, Hamoud Al; Sufyan, Hamad Al; Al-Tannir, Mohamad

doi:10.4172/2167-0250.1000126

Cited by 3 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…About 15% of infertile Saudi men had normal semen parameters despite high DFI; therefore, testing DFI in addition to other semen parameters is beneficial [ 38 ] as the DFI evaluation may reveal a hidden abnormality in apparently normal sperm parameters [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome

Omrani

Eisa

Javed³

et al. 2018

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundMale factor infertility is quite common as 30–50% of infertility cases are due to sperm defects. The high sperm DNA fragmentation is one of the causes of male factor infertility. Many factors cause sperm DNA fragmentation and could be testicular or post-testicular. The purpose of this study was to assess relationships among sperm DNA fragmentation, lifestyle factors and semen values of Saudi men and to determine impact of sperm DNA fragmentation on ICSI cycle outcome.MethodsThe duration of this study was from January 2015 to June 2016. The cases with female factor infertility were excluded. In total 94 couples were selected for investigation. The study parameters were male age, body mass index, smoking, semen values, % sperm DNA fragmentation, fertilization rate and pregnancy outcome. The ICSI procedure was performed in all patients per standard protocol. The semen samples were grouped based on % sperm DNA fragmentation into < 15%, 15–30 and > 30% which corresponded to low, moderate and high sperm DNA fragmentation, respectively.ResultsThere was no difference in ICSI outcome in low and moderate sperm DNA fragmentation, however, in high sperm DNA fragmentation no patient achieved pregnancy. In this study, 53.19% Saudi men had low, 32.98% moderate and 13.83% high DFI. Semen volume, sperm morphology and fertilization rate did not show any correlation trend with DNA fragmentation, however, sperm concentration and motility were negatively correlated in all DFI categories. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.ConclusionsThe results of this study indicated that 14% Saudi men had high DNA fragmentation. The BMI was positively correlated in moderate DFI category and smoking was positively correlated with low DFI category. The age was positively correlated in moderate and high DFI categories.

show abstract

Section: Discussionmentioning

confidence: 99%

Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome

Omrani

Eisa

Javed³

et al. 2018

Reprod Biol Endocrinol

View full text Add to dashboard Cite

show abstract

“…However, it has been reported that even among men with normal semen analysis parameters, there are cases of advanced SDF. 16 …”

Section: Introductionmentioning

confidence: 99%

Age, sexual abstinence duration, sperm morphology, and motility are predictors of sperm DNA fragmentation

Yoshiakwa‐Terada,

Takeuchi,

Tachibana

et al. 2024

Reprod Medicine & Biology

View full text Add to dashboard Cite

PurposeSperm DNA fragmentation (SDF) has recently received attention as a cause of male infertility. However, SDF cannot be fully assessed using conventional semen parameter evaluations alone. Therefore, the authors aimed to elucidate the relationship between SDF and sperm parameters via computer‐assisted sperm analysis (CASA) to improve treatment strategies in reproductive medicine.MethodsThis retrospective observational study analyzed the relationship between sperm parameters assessed by CASA and SDF values determined by the TUNEL assay in 359 patients who visited the Mie University Hospital for infertility treatment. The methodology involved semen analyses covering concentration, motility, and morphology, followed by SDF quantification using the flow cytometry.ResultsStatistical analysis revealed significant correlations between SDF and various factors, including age, sexual abstinence period, and specific CASA‐measured parameters. Notably, lower sperm motility rates and abnormal head dimensions were associated with higher SDF values, indicating that these parameters were predictive of SDF.ConclusionsThis study highlights the importance of sperm motility and head morphology as indicators of SDF, suggesting their usefulness in assessing male fertility. These findings demonstrate the efficacy of detailed sperm analysis, potentially increasing the success rate of assisted reproductive technologies by improving sperm selection criteria.

show abstract

“…However, the CASA results are susceptible to being compromised in low and high-concentration specimens, especially for morphological results, due to the heterogeneity between shapes of sperm either in one sample or across multiple samples from one subject [ 6 ]. Despite the strict criteria in the latest WHO laboratory manual for normal semen examination and evaluation [ 7 ], around 15% of infertile men were still diagnosed with normal semen [ 8 ]. By contrast, a positive correlation between sperm DNA fragmentation and reduced male fertility, even impaired offspring fertility, has been confirmed [ 9 ], in which the sperm DNA fragmentation index was recognized as a promising detection marker for male infertility diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications

Zhang

Guan

et al. 2022

Biosensors

View full text Add to dashboard Cite

Routine assessment of sperm DNA integrity involves the time-consuming and complex process of staining sperm chromatin. Here, we report a Raman spectroscopy method combined with extended multiplicative signal correction (EMSC) for the extraction of characteristic fingerprints of DNA-intact and DNA-damaged sperm cells directly on glass slides. Raman results of sperm cell DNA integrity on glass substrates were validated one-to-one with clinical sperm cell staining. Although the overall Raman spectral pattern showed considerable similarity between DNA-damaged and DNA-intact sperm cells, differences in specific Raman spectral responses were observed. We then employed and compared multivariate statistical analysis based on principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), and the classifications were validated by leave-one-out-cross-validation (LOOCV) and k-fold cross-validation methods. In comparison, the PLS-DA model showed relatively better results in terms of diagnostic sensitivity, specificity, and the classification rate between the sperm DNA damaged group and the DNA intact group. Our results demonstrate the potential of Raman based label-free DNA assessment of sperm cell on glass substrates as a simple method toward clinical applications.

show abstract

Sperm DNA Fragmentation in Saudi Infertile Men with Normal Standard Semen Parameters

Cited by 3 publications

References 11 publications

Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome

Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome

Age, sexual abstinence duration, sperm morphology, and motility are predictors of sperm DNA fragmentation

Micro-Raman Analysis of Sperm Cells on Glass Slide: Potential Label-Free Assessment of Sperm DNA toward Clinical Applications

Contact Info

Product

Resources

About