Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (<i>Sparus aurata</i>
 L., 1758) semen

Fabbrocini, Adele; D’Adamo, Raffaele; Pelosi, Sergio; Oliveira, Luis F.J.; Prete, Francesco Del; Silvestri, Fausto; Vitiello, Valentina; Sansone, Giovanni

doi:10.1111/jai.12726

Cited by 16 publications

(1 citation statement)

References 24 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For preservation of sperm during a longer period, cryopreservation is the best option, and protocols developed in fish species can keep the sperm quality up to several years (Fabbrocini et al, 2015). Moreover, sperm cryopreservation presents many other applications in broodstock management, including the transport of gametes from different centers, or preservation of selected genetic lines (Asturiano et al, 2017;Cabrita et al, 2010;Martínez-Páramo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

European eel sperm storage: Optimization of short-term protocols and cryopreservation of large volumes

et al. 2019

View full text Add to dashboard Cite

Maturation in captivity of European eel (Anguilla anguilla) requires long and costly hormonal treatments that often lead to asynchronic maturation between sexes. Therefore, optimization of sperm short-term storage methods and cryopreservation protocols can be a key factor for successful artificial fertilization. Two experiments were carried out to optimize the existing protocols.For the short-term storage experiment, sperm was diluted in P1 extender and then stored at different dilution ratios (1:9 and 1:49). The best outcome was then tested at different temperatures (4 and 20 ºC) and in constant agitation or still. In the cryopreservation experiments, large sperm volumes (cryotubes of 2 and 5 ml), different cooling rates (freezing tubes 1 or 3 cm above liquid nitrogen during 15 and 20 minutes), and different extender compositions (methanol 10% was used as cryoprotectant, and complemented with FBS 20%, BSA 5% or egg yolk 5%) were tested. Sperm kinetic parameters were analyzed with a CASA-Mot system both in fresh and short-or long-term stored samples.In the short-term storage trial, sperm quality did not show significant differences in the first 24 h after sperm collection between the different storage conditions tested. For longer time, 1:49 dilution ratio showed significantly better results than 1:9, and low temperature (4 ºC) was better for sperm preservation after 3 days.Cryopreserved sperm samples showed good motility results when they were frozen in cryotubes of 2 and 5 ml, with no significant differences compared to samples cryopreserved in lower volumes (straws of 0.5 mL). Furthermore, the combination of methanol (10%) and egg yolk (5%) as freezing medium, induced significant higher post-thawing motility values (over 50%) than the control (methanol 10%), whereas the addition of FBS (20%) and BSA (5%) led to a significant reduction of the sperm motility. The establishment of these storage and cryopreservation protocols will be important for the improvement of European eel artificial reproduction programs.

show abstract

Section: Introductionmentioning

confidence: 99%

European eel sperm storage: Optimization of short-term protocols and cryopreservation of large volumes

et al. 2019

View full text Add to dashboard Cite

show abstract

The effects of environmentally relevant concentrations of aldrin and methoxychlor on the testes and sperm of male Clarias gariepinus (Burchell, 1822) after short-term exposure

Neves

Barnhoorn

Wagenaar

2018

Fish Physiol Biochem

View full text Add to dashboard Cite

The organochlorine pesticides aldrin (0.14 μg/L) and methoxychlor (0.23 μg/L) were both present in the Albasini Dam, Limpopo Province, South Africa, during a field survey in 2014. The use of aldrin has been banned in the USA since 1987 and restricted in South Africa since 1992. The use of methoxychlor, however, remains undefined with little information available about its registration in South Africa despite being banned in Europe (2002) and USA (2003). The aim of this study was to determine the potential effects of environmentally relevant concentrations of aldrin and methoxychlor on the reproductive system of male catfish, Clarias gariepinus. Males were exposed for 96 h to the two pesticides under controlled laboratory conditions. Following exposure, each fish was weighed and measured, and a necropsy performed to determine any macroscopic abnormalities and the general health of the fish. The fish were killed and dissected and the testes removed, weighed and measured to determine the gonadosomatic index (GSI). The right testis of each fish was sectioned for histopathological assessment and to calculate the testes index (I ). The left testis was used for computer-assisted sperm analysis (CASA). The histopathological assessment of the testes showed histopathological changes such as of melano-macrophage centres (MMCs) and vacuolation of spermatogonia and spermatocytes. However, the classification of these changes indicated that the testes tissue structure was normal with slight histological changes. No statistically significant differences (p> 0.05) were found in the CASA parameters between exposure groups. The results of this study showed that the environmentally relevant concentrations of aldrin and methoxychlor did not have a negative effect on the motility of the mature sperm, but adverse effects were noted in the early stages of spermatogenesis, indicating possible effects over longer exposure periods.

show abstract

Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters

Medina‐Robles

Sandoval‐Vargas

Suárez-Martínez

et al. 2023

Aquaculture Reports

View full text Add to dashboard Cite

Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

Cited by 16 publications

References 24 publications

European eel sperm storage: Optimization of short-term protocols and cryopreservation of large volumes

European eel sperm storage: Optimization of short-term protocols and cryopreservation of large volumes

The effects of environmentally relevant concentrations of aldrin and methoxychlor on the testes and sperm of male Clarias gariepinus (Burchell, 1822) after short-term exposure

Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters

Contact Info

Product

Resources

About