Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Kashiwazaki, Naomi; Yamaguchi, Ryo; Uesugi, Ryuhei; Hishiyama, Nobuya; Kim, Masahide; Nakatsukasa, Ena; Kojima, Yoshihiko; Okuda, Yasushi; Hisamatsu, Shin; Inomata, Tomo; Shino, Masao

doi:10.1262/jrd.17052

Cited by 16 publications

(19 citation statements)

References 23 publications

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“…2006). On the contrary, we obtained better results regarding sperm motility and PMI compared with Kashiwasaki et al. (2005).…”

Section: Discussioncontrasting

confidence: 56%

“…Our post-thawing results for control and cooled sperm were lower for sperm motility, progressive status and PMI when compared with other studies using cat epididymal sperm (Axne´r et al 2004;Tebet et al 2006). On the contrary, we obtained better results regarding sperm motility and PMI compared with Kashiwasaki et al (2005). Since freeze-thaw protocols are considerably different among these studies, it is difficult to determine which factors may have influenced post-thaw viability.…”

Section: Discussionmentioning

confidence: 65%

“…2004) and 7% (Tebet et al. 2006)], while the lowest values were obtained with 3% glycerol (Kashiwasaki et al. 2005).…”

Section: Discussionmentioning

confidence: 96%

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Impact of 24‐h Cooling Prior to Freezing on the Survival of Domestic Cat (Felis catus) Epididymal Sperm

Martins

Villaverde

Lima

et al. 2009

Reprod Domestic Animals

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The aim of this study was to investigate the impact of a 24-h cooling period prior to freezing on domestic cat epididymal sperm viability. Fifteen tomcats were submitted to routine orchiectomy and sperm samples were retrieved from both epididymides in a Tris-glucose-20% egg yolk extender. For each tomcat, the diluted sperm was split into two equal volumes and cooled to 5 degrees C at a rate of 0.5 degrees C/min; one sample for 60 min (control) and the other for 24 h (cooled). After the cooling period, samples from both groups were frozen using an identical freezing protocol. Sperm samples were evaluated in three different periods: immediately after harvesting, after cooling at 5 degrees C for 24 h (cooled group) and after freezing-thawing of control and cooled groups. Evaluations consisted of sperm motility and progressive status, sperm morphology and plasma membrane integrity (PMI) using two fluorescent probes. After cooling for 24 h, a decrease (p < 0.05) in sperm motility, progressive status and PMI was observed when compared to sperm samples immediately after collection. Comparing the results obtained after thawing, no difference (p < 0.05) was found regarding sperm motility, progressive status, PMI and sperm morphology between control and cooled groups. The results from the present study show that cooling cat epididymal spermatozoa at 5 degrees C for 24 h prior to freezing does not lead to major damage of spermatozoa impairing the freeze-thaw process.

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“…2006). On the contrary, we obtained better results regarding sperm motility and PMI compared with Kashiwasaki et al. (2005).…”

Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 65%

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Impact of 24‐h Cooling Prior to Freezing on the Survival of Domestic Cat (Felis catus) Epididymal Sperm

Martins

Villaverde

Lima

et al. 2009

Reprod Domestic Animals

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“…It has been reported that a pregnancy rate of only about 10% was obtained in the domestic cat after the use of frozen-thawed semen with vaginal insemination (Tsutsui et al, 2003). The fertilizing ability of frozenthawed epididymal spermatozoa has been proven (Kashiwazaki et al, 2005). Cryopreservation of epididymal spermatozoa is a potentially valuable procedure for banking genetic resource from individuals of endangered species that die accidentally, (Tsutsui et al, 2003) a conception rate of 27.3% for eleven female cats that received unilateral intrauterine AI of 5 × 10 7 sperm has been reported (Nakatsukasa et al, 2000, Hay & Goodrowe, 1993.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction

Cocchia¹,

Ciani

El-Rass

et al. 2009

Zygote

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Cryopreservation of gametes is an important tool in assisted reproduction programmes; long-term storage of oocytes or spermatozoa is necessary when in vitro fertilization (IVF) or artificial insemination is to be performed at a future date. Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of endangered populations. The objectives of this work were to: (1) examine sperm motility, viability, abnormality and acrosome integrity of frozen-thawed domestic cat epididymal spermatozoa; and (2) evaluate the same cryopreservation method on wild feline spermatozoa, needed to preserve their genetic resources. Epididymides were collected from 20 domestic cats during routine neutering procedure and from two wild felines at autopsy. The sperm samples, diluted with 4% glycerol/Tris/egg yolk, were loaded into 0.25 ml mini-straws, exposed to nitrogen vapour and stored in liquid nitrogen. After 4 weeks, samples were thawed and re-evaluated. The quality of each fresh and frozen-thawed sperm sample was tested by determining the motility (54.7 ± 11.3% and 32 ± 13.1% respectively for cat spermatozoa; 38.3 ± 18.7% and 21.5 ± 16.8% respectively for tiger spermatozoa), viability (74.3 ± 8.6% and 45.2 ± 9.4% respectively for cat spermatozoa; 42.4 ± 14.5% and 33.5 ± 12.9% respectively for wild felid spermatozoa), morphology and acrosomal status. The present study showed that feline epididymal spermatozoa can be frozen in egg-yolk extender with 4.0% glycerol in 0.25 ml straws. The procedure used in the present study for epididymal cat sperm cryopreservation may be applied to bank the genetic resources of wild felid species.

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“…Several studies have demonstrated that the domestic cat may be used as an important experimental and useful model to establish assisted reproductive technologies in wild cats, such as, for instance, artificial insemination, in vitro fertilization, embryo transfer, and embryo/sperm cryopreservation (Kashiwaki et al, 2005; Pelican et al, 2006; Nagano et al, 2008). However, all these techniques have some limitations once the gametes are terminally differentiated (haploid cells) and are available in a finite number.…”

mentioning

confidence: 99%

Germ Cell Transplantation in Felids: A Potential Approach to Preserving Endangered Species

Silva

Costa

Lacerda

et al. 2012

Journal of Andrology

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With the exception of the domestic cat, all members of the family Felidae are considered either endangered or threatened.Although not yet used for this purpose, spermatogonial stem cell (SSC) transplantation has a high potential to preserve the genetic stock of endangered species. However, this technique has not previously been established in felids. Therefore, we developed the necessary procedures to perform syngeneic and xenogeneic SSC transplants (eg, germ cell [GC] depletion in the recipient domestic cats, enrichment and labeling of donor cell suspension, and the transplantation method) in order to investigate the feasibility of the domestic cat as a recipient for the preservation and propagation of male germ plasm from wild felids. In comparison with busulfan treatment, local x-ray fractionated radiation was a more effective approach to depleting endogenous spermatogenesis. The results of both syngeneic and xenogeneic transplants revealed that SSCs were able to successfully colonize and differentiate in the recipient testis, generating elongated spermatids several weeks posttransplantation. Specifically, ocelot spermatozoa were observed in the cat epididymis 13 weeks following transplantation. As donor GCs from domestic cats and ocelots were able to develop and form mature GCs in the recipient environment seminiferous tubules, these findings indicate that the domestic cat is a suitable recipient for SSC transplantation. Moreover, as modern cats descended from a medium-size cat that existed approximately 10 to 11 million years ago, these results strongly suggest that the domestic cat could be potentially used as a recipient for generating and propagating the genome of wild felids.

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Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Cited by 16 publications

References 23 publications

Impact of 24‐h Cooling Prior to Freezing on the Survival of Domestic Cat (Felis catus) Epididymal Sperm

Impact of 24‐h Cooling Prior to Freezing on the Survival of Domestic Cat (Felis catus) Epididymal Sperm

Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction

Germ Cell Transplantation in Felids: A Potential Approach to Preserving Endangered Species

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