Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele

Blumer, Camile Garcia; Fariello, R.M.; Restelli, Adriana Ester; Spaine, D.M.; Bertolla, Ricardo Pimenta; Cedenho, A.P.

doi:10.1016/j.fertnstert.2007.09.007

Cited by 140 publications

(131 citation statements)

References 44 publications

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“…It was presumabe that as the protein expression was elevated after varicocelectomy; mean of sperm motility was increased. Some reports have indicated that defects in mitochondrial proteins play important roles in varicocele [21].…”

Section: Discussionmentioning

confidence: 99%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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Purpose To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. Methods This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. Results Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). Conclusions High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.

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Section: Discussionmentioning

confidence: 99%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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“…There is a strong association between decreased sperm motility and oxidative stress [11,12,28,29]. Sperm motility is generated through constant ATP production by mitochondria localized in the sperm midpiece.…”

Section: Discussionmentioning

confidence: 99%

“…Although the other mechanisms are important, in our study we selected a test which assessed the third stage, when excess ROS will directly interact with DNA and cause single and doublestrand non-specific breaks in the DNA molecule [28]. Also, as a more sensitive test, the Comet assay will allow analysis of very initial lesions to the DNA.…”

Section: Discussionmentioning

confidence: 99%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

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Purpose To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria. Methods Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria. Semen was processed using Percoll, and motility, DNA fragmentation, and mitochondrial activity were analyzed pre-and post-processing. Results No differences were observed in age, abstinence, volume, sperm morphology, progressive motility, concentration, and vitality (p>0.10). Variables were grouped according to time (pre-vs post-processing) and group (leukocytospermia vs non-leukocytospermia) because no interactions could be observed. Leukocytospermia was associated to increased DNA fragmentation, while semen processing led to a decrease in DNA fragmentation and to increased mitochondrial activity. Conclusion While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation. Semen processing also selects sperm with higher mitochondrial activity.

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“…Excessive ROS production, associated to increased testicular temperature, typical condition of varicocele, and to abnormal retention of cytoplasmic droplets, due to an impaired spermatogenesis, causes a reduced/altered sperm functions correlated with DNA damages. Studies confirm higher levels of damaged and fragmented DNA ROS related and thus reduced MMP and decreased sperm motility (39), in infertile men with varicocele compared to fertile men without varicocele (40-41).…”

mentioning

confidence: 85%

Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

Scarselli

Lobascio

Terribile

et al. 2016

Arch Ital Urol Androl

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The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients’ ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration > 2 million/ml and progressive motility < 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS). Afterwards, the sperm progressive motility was assessed using Computer Assisted Sperm Analysis (CASA system). Incubation with MYO-Inositol improved sperm progressive motility in high viscosity samples compared to control group (38.9% ± 3.0 vs 24.35% ± 2.41, respectively; p ≤ 0.0001). Conversely, no statistically significant difference was observed in total sperm progressive motility in varicocele samples compared with control group (22.7% ± 2.07 vs 26.7% ± 3.31, respectively; p = 0.085). The MYO-Inositol positive effect on spermatozoa motility may depend on the type of sperm damage: heavy structural and biochemical defects which typically affects patients with varicocele are not restored by Inositol. On the contrary, MYOInositol is able to improve sperm motility in semen samples with high viscosity, since those samples show no substantial structural sperm defects.

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Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele

Cited by 140 publications

References 44 publications

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

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