Sperm quality variables as indicators of bull fertility may be breed dependent

Morrell, Jane M.; Nongbua, Thanapol; Valeanu, Sabina; Verde, Isabel Lima; Lundstedt‐Enkel, Katrin; Edman, Anders; Johannisson, Anders

doi:10.1016/j.anireprosci.2017.08.001

Cited by 48 publications

(32 citation statements)

References 23 publications

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“…This might point to differences between breeds in their seasonal variations in sperm quality, or to differences due to the stallion´s location during semen collection. In bulls, breed was shown to affect which parameters are useful for prediction of fertility [30]. Aurich concluded that because of small variations in semen quality between seasons, the choice of stallion is more important than the season of collection, and our results support this conclusion [14].…”

Section: Discussionsupporting

confidence: 76%

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Johannisson

Al-Essawe

Al-Saffar

et al. 2020

Journal of Reproduction and Development

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The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.

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Section: Discussionsupporting

confidence: 76%

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Johannisson

Al-Essawe

Al-Saffar

et al. 2020

Journal of Reproduction and Development

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“…Since part of the head structures of spermatozoa are equipped with essential enzymes for penetrating oocytes (Layek et al, 2016), intact acrosomal cap is crucial condition for fertilization. Acrosomal integrity, therefore, could become the best evaluation parameter for fertilization (Morrell et al, 2017). However, the effects of β-carotene addition in semen diluents on acrosomal integrity have not been widely reported.…”

Section: Discussionmentioning

confidence: 99%

“…The most significant obstacle to utilize cryopreserved semen of many species is that cooling, freezing, and thawing damage sperm membrane structures, leading to a fewer number of viable and motile cells after thawing process (Morrell et al, 2017). Research on the damage of spermatozoa after freezing and thawing have been previously reported on some domestic animals such as bull (Ting-xi et al, 2016), ram (Toker et al, 2017), stallion (Krakowski et al, 2013), and boar (Yeste et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene.

Eriani

Azhar

Ihdina

et al. 2018

Trop. Anim. Sci. J.

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Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.

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“…Seiring dengan bertambahnya umur, pejantan akan menghasilkan semen dengan volume yang cenderung meningkat (Dewi, Ondho, & Kurnianto, 2012;Wahyuningsih, Saleh, & Sugiyatno, 2013), motilitas menurun dan meningkatnya jumlah spermatozoa yang abnormal (Brito et al, 2002). Kualitas semen dari seekor pejantan berhubungan erat dengan fertilitas (Mishra, Palai, Sarangi, Prusty, & Maharana, 2013;Morrell et al, 2017) dan memiliki arti ekonomis yang tinggi pada program produksi ternak (Han & Peñagaricano, 2016).…”

Section: Pendahuluanunclassified

“…Hal demikian menjadi salah satu pertimbangan karena pada proses pembekuan semen, motilitas sperma akan mengalami penurunan kurang lebih 20 -30% pada sapi Bali (Hapsari, Khalifah, Widyas, Pramono, & Prastowo, 2018). Seperti yang dilaporkan pada penelitian-penelitian sebelumnya, motilitas memiliki korelasi positif terhadap fertilitas pejantan (Januskauskas, Johannisson, & Rodriguez-Martinez, 2001;Morrell et al, 2017). Secara umum diperoleh rata-rata persentase L/D spermatozoa sebesar 71,95±7,90%.…”

Section: Hasil Dan Pembahasan Data Kualitas Semen Segar Sapi Baliunclassified

Kualitas semen segar sapi Bali (Bos javanicus) pada kelompok umur yang berbeda

Prastowo

Dharmawan²,

Nugroho³

et al. 2018

JIT

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Penelitian ini bertujuan untuk mengetahui kualitas semen segar sapi Bali pada kelompok umur yang berbeda. Sampel yang digunakan berupa semen segar dari 8 pejantan sapi Bali di Balai Besar Inseminasi Buatan (BBIB) Singosari Malang, Jawa Timur, terbagi menjadi 2 kelompok umur yaitu 4 dan 7 tahun. Rata-rata bobot badan sapi pada kelompok umur 4 tahun adalah 656,75±32,69 Kg, dan lingkar skrotum 27,5±1,64 cm sedangkan pada kelompok umur 7 tahun adalah 615,5±72,59 Kg dan 27,93±0,74 cm. Kualitas semen segar diamati dari 10 kali penampungan menggunakan vagina buatan. Parameter yang diamati meliputi volume (ml), pH, konsentrasi (x106/ml), motilitas (%), persentase spermatozoa hidup (L/D; %), abnormalitas primer (%) dan abnormalitas sekunder (%). Untuk mengetahui perbedaan antar 2 kelompok umur, data kualitas semen dibandingkan menggunakan t-test. Hasil penelitian menunjukkan bahwa pada kelompok umur 4 dan 7 tahun masing-masing diperoleh volume 4,55±0,91 ml dan 5,18±1,58 ml, pH 6,51±0,06 dan 6,52±0,01, konsentrasi 962,30±390,50×106/ml dan 1079,00±90,56×106/ml, L/D 71,88±2,58% dan 72,02±1,35%, motilitas 68±3,11% dan 66,04±6,30%, abnormalitas primer 1,054±0,20% dan 0,93±0,14% serta abnormalitas sekunder 3,54±0,48% dan 4,24±0,31%. Volume dan abnormalitas sekunder secara statistik menunjukkan adanya perbedaan yang nyata (P<0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa pada kelompok pejantan sapi Bali umur 7 tahun menghasilkan volume semen dan abnormalitas sekunder lebih tinggi dibandingkan umur 4 tahun.Kata kunci: sapi Bali, pengaruh umur, kualitas semen segar

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Sperm quality variables as indicators of bull fertility may be breed dependent

Cited by 48 publications

References 23 publications

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene.

Kualitas semen segar sapi Bali (Bos javanicus) pada kelompok umur yang berbeda

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