Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)

Costa, Laiana Cristina da; Azeredo-Oliveira, Maria Tercília Vilela de; Tartarotti, Ester

doi:10.1590/s1415-47572008000300008

Cited by 21 publications

(14 citation statements)

References 27 publications

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“…10 The analysis of the nucleolar behavior during the meiosis of these bugs has highlighted that all species feature the nucleolar persistence phenomenon. Therefore, as the observed results were the same for all triatomines, we represent the Our results are in agreement with the initial works carried out for the Triatoma, [11][12][13][14][15][16][17] Meccus, 18 Rhodnius, 9,19 and Panstrongylus 9 genera, which likewise have exhibited this phenomenon during spermatogenesis of these vectors.…”

supporting

confidence: 92%

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Madeira

Borsatto

Lima

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. All species of triatomines are considered potential vectors of Chagas disease and the reproductive biology of these bugs has been studied by different approaches. In 1999, nucleolar persistence during meiosis was observed in the subfamily for the first time. Recently, it has been observed that all species within the genus Rhodnius exhibit the same phenomenon, suggesting that it may be a synapomorphy of the triatomines. Thus, this article aims to analyze the nucleolar behavior during spermatogenesis of 59 triatomine species. All analyzed species exhibited nucleolar persistence during meiosis. Recently, it has been suggested that nucleolar persistence may be fundamental for the spermatogenesis of these vectors, since it is related to the formation of the chromatoid body. Therefore, we emphasize that this phenomenon is a peculiarity of the Triatominae subfamily and that further studies are required to analyze whether the nucleolar material that persists is active.The Triatominae subfamily is composed of 150 species (148 living species and two fossils), grouped into 18 genera and five or six tribes. All triatomine species are considered potential vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease. 1Chagas disease is a neglected disease that has no cure. The main way to minimize the incidence of this disease in human populations is through vector control.1 It is estimated that more than 5 million people are infected by T. cruzi, the parasite that causes Chagas disease, and that 70 million still live at risk, which places this illness among the most serious parasitic diseases in the world.1 Thus, improving the knowledge on several fields on triatomine vector potentiality (such as ecology 2 and biology 3 ) may provide important information for control measures.The reproductive biology of these bugs has been studied by different approaches, such as cytogenetic, 4 structural, 5 and ultrastructural 6 analysis. Furthermore, the spermatogenesis of the triatomines is characterized as cystic, 5,6 and it has been suggested that, during imaginal molt (transition from the fifth instar nymph to adult), the cell division is disrupted, aiming to reduce energy costs, and the differentiation into sperm is stimulated to ensure the paternity of the adult male. 7In 1999, Tartarotti and Azeredo-Oliveira, 8 while studying the spermatogenesis of Panstrongylus megistus and Panstrongylus herreri (= Panstrongylus lignarius), noted that these triatomines exhibited a different nucleolar behavior than the one described for other eukaryotes: the nucleolus persisted during all stages of meiosis. The authors characterized this phenomenon as nucleolar persistence.Recently, Alevi and others 9 have observed that all species within the genus Rhodnius feature nucleolar persistence during meiosis as well, and they suggested that analyses of the nucleolar behavior should be carried out in a large range of species of triatomines to examine whether that characteristic is a synapomorphy of the Triatominae subfamily.Thus, this ...

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supporting

confidence: 92%

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Madeira

Borsatto

Lima

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…The phenomenon of nucleolar persistence during spermatogenesis is characterized by the presence of the nucleolus or nucleolar corpuscles during meiotic metaphase, since, generally, in eukaryotes the nucleolus is fragmented in late prophase and is only reorganized at the end of prophase. This phenomenon has been described in 21 species of triatomines (Tartarotti and Azeredo-Oliveira, 1999;Azeredo-Oliveira, 2004, 2007;Severi-Aguiar and Azeredo-Oliveira, 2005;Severi-Aguiar et al, 2006;Costa et al, 2008;Alevi et al, 2013e, 2014d, and Alevi et al (2014d) have proposed that it is a synapomorphy of the subfamily Triatominae.…”

Section: Discussionmentioning

confidence: 86%

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Pereira¹,

Alevi²,

Mendonca³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study describes spermatogenesis in Triatoma vandae and the nucleolar behavior of T. vandae and Triatoma williami, with a cytotaxonomic focus. Analysis of mitotic and meiotic metaphases of T. vandae confirms the species karyotype. T. vandae presents some characteristics during meiosis that differentiate it from T. williami, including the presence of a chromocenter with two sex chromosomes individualized during early prophase, and the presence of a bi-or tripartite corpuscle in polyploid nuclei. It was possible to observe the compaction of chromatin during prophase resulting in holocentric chromosomes. During metaphase, the autosomes presented a ring shape and the sex chromosomes were in the center of the ring. These chromosomes were separated in anaphase. Although it is common, we did not observe the phenomenon of late migration of the sex chromosomes. By means of silver ion impregnation it was possible to describe nucleologenesis in T. vandae and T. williami.

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“…This phenomenon differs from the more general process in which the disorganization of the nucleolus occurs during prophase in that, from diakinesis until the end of the meiotic division, it is not visible, suggesting that at the time it is already completely disorganized (Risueño and Medina, 1976). Nucleolar persistence, that is, the maintenance of visible nucleolar material after meiotic prophase, has been described in several Heteroptera species (Morielle-Souza and Azeredo-Oliveira, 2007;Costa et al, 2008;Souza et al, 2008). In Mormidea v-luteum, Oebalus poecilus and Oebalus ypsilongriseus (three Heteroptera species from the Pentatomidae family) the nucleolar material could be observed as a round corpuscle until the end of telophase I, and the round spermatids still exhibited a variable number of small nucleoli.…”

Section: Introductionmentioning

confidence: 98%

Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae

Arakaki¹,

Souza²,

Castanhole³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The nucleolar material of Chariesterus armatus was analyzed during spermiogenesis in cell preparations impregnated with silver nitrate. Nucleolar corpuscles were observed in spermatids at the beginning of the process, showing that this organoid is also maintained after meiosis. In addition, nucleoli were seen in the round spermatids connected to the X-chromosome (bearer of the nucleolar organizer in C. armatus), indicating de novo synthesis of nucleolar material. This differs from the reorganization of ribosomal granules, transported from meiotic spermatocytes to round spermatids, where they would support protein synthesis, which is reported for other species. We also observed connections of nucleolar corpuscles to the nuclear membrane regions where the tail and the acrosome will be formed, suggesting close involvement of the nucleolar material in the formation of these structures. In addition to the nucleolar bodies, we detected silver-positive structures, which will require new approaches to clarify their role. One of these structures, observed in the cytoplasm, appears to correspond to the chromatoid body, which has been found in several organisms, but is still poorly understood; another is a complex structure to which the tail appears to be connected. We conclude that C. armatus is an appropriate model for understanding not only the synthesis of rRNA in the spermiogenesis, but also the functional meaning of the close relationship of nucleolar material with other structures during this process.

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Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)

Cited by 21 publications

References 27 publications

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)

Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae

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