Spermatogenesis in fish

Schulz, Rüdiger; França, Luiz R.; Lareyre, Jean‐Jacques; Legac, Florence; Chiarini-Garcia, Hélio; Nóbrega, Rafael Henrique; Miura, Takeshi

doi:10.1016/j.ygcen.2009.02.013

Cited by 1,045 publications

(915 citation statements)

References 184 publications

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“…Such discrepancies warrant further studies to confirm the conserved role of DMRT1 in spermatogenesis of teleosts. Previously, we confirmed that estrogen has an important role in the spermatogenesis of three-spot wrasse (Kobayashi et al 2011) similar to other teleosts (Schulz et al 2010). Indeed, AI treatment to IP male inhibited the renewal and proliferation of spermatogonia, corollary of the ratio of spermatocyte and spermatozoa were increased (Kobayashi et al 2011).…”

Section: Discussionsupporting

confidence: 83%

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

et al. 2014

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Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes.Keywords DMRT1 Á Spermatogenesis Á Efferent duct Á Wrasse Á Aromatase inhibitor Á Sertoli cell Abbreviations DMRT1 Doublesex-mab 3-related transcription factor 1 E2

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Section: Discussionsupporting

confidence: 83%

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

et al. 2014

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“…During this stage genetic diversity is generated by meiosis II, the result of which is four haploid cells, and the formation of spermatids. T and 11-KT are also involved in this process of maturation (Schulz et al, 2010). These correlations found in S3 can be explained by the fact that 16:0 and consequently 16:1, are the main products of the de novo synthesis of the fatty acids (Cook and Mc Master, 2002) and as we know, fatty acids, especially polyunsaturated fatty acids, are essential in the formation of sperm cell membranes (Whates et al, 2007).…”

Section: Discussionmentioning

confidence: 92%

“…They showed that not only is it a factorin the regulation of final maturation, but also in the early stages of spermatogenesis, especially the initiation of meiosis. Schulz et al (2010) also suggested that DHP is an essential hormone in the initiation of meiosis during spermatogenesis in teleost fish. In our experiment, the lowest plasma levels of the progestin DHP were registered in S1 and increased significantly after the growing phase (S2), reaching their highest concentrations in the maturation (S3) and spermiation (S4-S5) phases.…”

Section: Discussionmentioning

confidence: 99%

Exploring correlations between sex steroids and fatty acids and their potential roles in the induced maturation of the male European eel

et al. 2015

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ElsevierBaeza Ariño, R.; Peñaranda, D.; Vilchez Olivencia, MC.; Tveiten, H.; Pérez Igualada, LM.; Asturiano Nemesio, JF. (2015). Exploring correlations between sex steroids and fatty acids and their potential roles in the induced maturation of the male European eel. Aquaculture. 435:328-335. doi:10.1016Aquaculture. 435:328-335. doi:10. /j.aquaculture.2014 . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 AbstractThe present study was undertaken to evaluate the correlations between the fatty acids in the liver and testis and the plasma levels of the hormonal steroids used during eel spermatogenesis, in order to clarify the physiological roles fatty acids play in the spermatogenetic process. The stages of testis development (S1-S5) were assessed by histological observations in order to classify the different phases of hormonally-induced spermatogenesis and evaluate the possible relationships between the hormones and fatty acids in each stage.The highest plasma levels of 17β-Estradiol (E2), testosterone (T) and 11-ketotestosterone (11KT) were found in S1, when spermatogonial proliferation occurs. A correlation was found between 17α-20β-dihydroxy-4-pregnen-3-one (DHP) levels and some fatty acids during the proliferation and growing phases (S1-2), suggesting that DHP might modulate lipid metabolism in the liver during early spermatogenesis. The DHP levels increased significantly during the growing phase (S2) and remained at high levels throughout the subsequent development stages (S3-S5).Similar to results found in mammals, our results show that in the eel there are regulatory mechanisms, including eicosapentaenoic acid (20:5-n3, EPA) and docosahexaenoic acid (22:6-n3, DHA), which act as modulators in the synthesis of androgens, particularly during the final phase of sperm maturation. Our results suggest that the fact that EPA, ARA and DHA concentrations in the eel testis remain constant/stable during spermiation could be related to the subsequent union of the spermatozoa and the egg.The findings from this research provide new insights for further studies about the possible effect of steroids on desaturase activity and highlight the importance of the effect of lipid metabolism during male eel spermatogenesis.

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“…However in the past the role played by 17,20β-P in males remained unclear and critical revision articles were written regarding its activity as a maturation-inducing steroid in females (Nagahama, 1987). Moreover, the involvement of 17,20β-P regulating different phases of teleost fish testes development has been highlighted (Schulz et al, 2010;Scott et al, 2010). The 17,20β-P testes synthesis and release in male blood plasma has been studied in more than 100 species as per Scott et al (2010) revision article.…”

Section: Introductionmentioning

confidence: 99%

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Cantonnet¹,

Mateo²,

Alberro³

et al. 2015

Neotrop. ichthyol.

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The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3 H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,17, were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature testes and in those at the first spermatogenesis stages, while a clear increase was observed during the whole spermatogenesis and spermiation phases (from undetectable to 1047 pg mL -1 ).In vitro studies together with plasma detection suggest that 17,20β-P is a good steroid candidate involved in M. furnieri testes maturation. The role of cortisol during late phases of testes development needs further studies.El objetivo fue investigar cuales eran los esteroides C21 más importantes producidos por los testículos en espermiación de la corvina blanca Micropogonias furnieri (Sciaenidae) para poder identificar los potenciales mediadores de la maduración gamética de los machos de esta especie. Los esteroides producidos por los testículos en espermiación fueron identificados después de su incubación con el precursor 3 H-17-hidroxi-4-pregnene-3,20-diona a través de cromatografía de capa fina y cromatografía líquida de alta presión y posteriormente por oxidación enzimática, acetilación e inmunoquímica. Los principales metabolitos producidos por los testículos en espermiación fueron la 17,20β-Dihidroxi-4-pregnen-3-ona (17,20β-P) y la 11β,17,21-Trihidroxi-4-pregnene-3,20-diona (cortisol). Contrariamente a lo esperado, no se encontró el derivado tri-hidroxilado de la progesterona llamado 17,20β,21-Trihidroxi-4-pregnen-3-ona. Los niveles circulantes de 17,20β-P fueron no detectable en los testículos inmaduros y en aquellos en inicios de espermatogénesis, mientras que un aumento claro en las concentraciones circulantes fue detectada en corvinas en plena espermatogénesis y en espermiación (desde no detectable a 1047 pg mL -1 ). Los resultados obtenidos in vitro junto a los cambios a nivel plasmático sugieren que la 17,20β-P es un buen candidato a proponer como esteroide involucrado en la regulación del proceso de maduración testicular de la corvina. La función del cortisol a nivel testicular debería ser mejor estudiada en las etapas finales del desarrollo testicular de esta especie.

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Spermatogenesis in fish

Cited by 1,045 publications

References 184 publications

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

Exploring correlations between sex steroids and fatty acids and their potential roles in the induced maturation of the male European eel

17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

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