Spermatozoa with numerical chromosomal abnormalities are more prone to be retained by Annexin V‐<scp>MACS</scp> columns

Esbert, Marga; Godo, Anna; Soares, Sérgio Reis; Florensa, M.; Amorós, David; Ballesteros, A.; Vidal, Francesca

doi:10.1111/andr.12376

Cited by 17 publications

(17 citation statements)

References 36 publications

(61 reference statements)

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“…Similar to these results, Zhang et al (2018) reported that the combination of DCG followed by AV-MACS in immotile sperm samples resulted in the recovery of a sperm population with lower DNA fragmentation compared to DGC alone. In agreement, Esbert et al (2017) reported that DGC followed by AV-MACS reduced the number of spermatozoa porting chromosomal abnormalities.…”

Section: Sperm Selection Based On Membrane Characteristicssupporting

confidence: 69%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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Almost 50% of the infertility cases are due to male factors. Assisted reproductive technologies (ARTs) allow to overcome the incapacity of these patients' spermatozoa to fertilize the oocyte and produce a viable and healthy offspring, but the efficiency of the different techniques has still the potential to improve. According to the latest reports of the European Society of Human Reproduction and Embryology (ESHRE) and the Centers for Disease Control and Prevention of the United States (CDC), the percentages of deliveries per ART cycle in 2014 and 2016 were 21 and 22%, respectively. Among the reasons for this relatively low efficiency, the quality of the spermatozoa has been pointed out as critical, and the presence of high percentages of DNA-damaged spermatozoa in patients' ejaculates is possibly one of the main factors reducing the ARTs outcomes. Thus, one of the main challenges in reproductive medicine is to ensure the highest quality of the spermatozoa used in ARTs, and specifically, in terms of genetic integrity. The latest techniques for the preparation and selection of human spermatozoa are herein discussed focusing on those proven to improve one or several of the following parameters: sperm genetic integrity, fertilization capacity, embryo production, and in vitro survival, as well as pregnancy and delivery rates following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). In addition, we discuss the potential of techniques developed in non-human mammals that could be further transferred to the clinic.

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Section: Sperm Selection Based On Membrane Characteristicssupporting

confidence: 69%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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“…These studies reported that processing samples through MACS decreased sperm aneuploidy rates in the annexin V (-) fraction. Moreover they compared the aneuploidy rate in the annexin V (-) and V (+) fractions and concluded that aneuploid sperm cells are preferentially retained in the MACS column (Esbert et al 2017). Our study is the first of its kind to evaluate the rate of normal or balanced spermatozoa and unbalanced spermatozoa after MACS in the two fractions of annexin V (-) and annexin V (+) for patients carrying a constitutional structural chromosomal abnormality.…”

Section: Discussionmentioning

confidence: 90%

“…To the best of our knowledge, only two studies have worked on the effects of MACS on sperm aneuploidy rate in patients with a normal karyotype (Vendrell et al 2014;Esbert et al 2017). These studies reported that processing samples through MACS decreased sperm aneuploidy rates in the annexin V (-) fraction.…”

Section: Discussionmentioning

confidence: 99%

Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality

et al. 2019

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Background: We showed that in men with a constitutional chromosomal abnormality, DNA fragmentation was significantly higher in chromosomally unbalanced spermatozoa than in spermatozoa with a normal or balanced chromosomal content. These results could be explained by a phenomenon already described in infertile men: abortive apoptosis. Objectives: To determine if magnetic-activated cell separation could select spermatozoa with lower levels of DNA fragmentation and unbalanced chromosome content in men carrying a structural chromosomal abnormality. Materials and methods: The spermatozoa of ten males with a chromosomal rearrangement were separated into two populations using MACS (annexin V (-) and annexin V (+) fractions), in order to study meiotic segregation by FISH, the percentage of spermatozoa with an externalization of phosphatidylserine (EPS) by annexin V staining and DNA fragmentation by TUNEL on the whole ejaculate and on selected spermatozoa in the same patient. Results: For all patients, the percentage of spermatozoa with EPS decreased in the annexin V (-) fraction and increased in the annexin V (+) fraction as compared to the frozen-thawed semen sample. The rates of DNA fragmentation were statistically much lower in the annexin V (-) fraction when compared to the rate before MACS for all but one patient. Conversely, we observed a statistically significant higher rate of DNA fragmentation in the annexin V (+) fraction for 6 patients. After MACS, there was a significant increase of normal/balanced spermatozoa in the fraction of annexin V (-) for all patients. Conversely, we observed a significant decrease in the fraction of annexin V (+) for seven patients. Discussion and Conclusions: MACS is a promising tool for increasing the selection of healthy spermatozoa, with a decrease in the number of spermatozoa with EPS, DNA fragmentation and chromosome unbalance, for use in assisted reproductive technologies such as ICSI for males with a chromosomal structural abnormality.

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“…Colloidal microbeads conjugated with annexin V effectively separated spermatozoa with numerical chromosomal aberrations and apoptotic spermatozoa (Esbert et al, 2017) and this procedure can be applied to select spermatozoa needed for the ICSI procedure. Colloidal microbeads conjugated with annexin V effectively separated spermatozoa with numerical chromosomal aberrations and apoptotic spermatozoa (Esbert et al, 2017) and this procedure can be applied to select spermatozoa needed for the ICSI procedure.…”

Section: Apoptosis and Testicular Cadmium Levelmentioning

confidence: 99%

“…In men with varicocele, elevated levels of apoptosis were found in spermatozoa. Colloidal microbeads conjugated with annexin V effectively separated spermatozoa with numerical chromosomal aberrations and apoptotic spermatozoa (Esbert et al, 2017) and this procedure can be applied to select spermatozoa needed for the ICSI procedure.…”

Section: Apoptosis and Testicular Cadmium Levelmentioning

confidence: 99%

A global view of the pathophysiology of varicocele

2018

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Varicocele is the most common abnormality identified in men being evaluated for subfertility. In this comprehensive review of the pathophysiology of varicocele, we will shed light on novel pathophysiological findings and their clinical implications that may direct future researches; we will shed light on the impact of transient scrotal hyperthermia and the roles of inflammation and differential protein expression and androgen expression in spermatozoa on inducing pathophysiological findings. Furthermore, we will clarify the linked processes contributing to the pathophysiology of varicocele and the impact of genetics on the induction of these processes. Spermatogenesis is a temperature-sensitive process, and heat stress of varicocele is considered the most plausible cause of impaired spermatogenesis. The three processes associated with the presence of varicocele - heat stress, excess reactive oxygen species, and increased apoptosis - appear to be linked; heat stress is associated with increased levels of reactive oxygen species and oxidative stress, which can induce apoptosis. The genetic role should not be overlooked as a contributing factor in the induction of heat stress, excess reactive oxygen species/oxidative stress, and apoptosis; this is evidenced by the association of varicocele with decreased expression of heat-shock proteins, higher polymorphism of glutathione S transferase and nitric oxide synthase genes, and increased BAX and decreased BCL2 genes and proteins. In this article, we will highlight the need of application of novel diagnostic techniques that can provide a precise pathophysiological diagnosis to guide potential specific innovative therapies. Innovative therapies can counteract the varicocele-induced stasis, suppress the degenerative effects of testicular hyperthermia, reduce the varicocele-induced apoptosis, and target the elevated-neutrophil products aiming at abrogating the testicular damage caused by the induced varicocele in rats/mice. In conclusion, on the basis of the novel scientific research, it may be possible to formulate new treatments and achieve the appropriate selection of patients who can benefit from these treatments.

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Spermatozoa with numerical chromosomal abnormalities are more prone to be retained by Annexin V‐MACS columns

Cited by 17 publications

References 36 publications

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality

A global view of the pathophysiology of varicocele

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