Spermiogenesis Deficiency in Mice Lacking the
            <i>Trf2</i>
            Gene

Zhang, Di; Penttilä, Tarja-Leena; Morris, Patricia L.; Teichmann, Martin; Roeder, Robert G.

doi:10.1126/science.1059188

Cited by 189 publications

(175 citation statements)

References 27 publications

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“…68 Unlike TRF2 in Drosophila, zebrafish and Xenopus, mouse TRF2 is not required for embryonic development. 79,80 TRF2 is widely expressed in the adult animal. [61][62][63]65 Mouse TRF2 is essential for spermiogenesis.…”

Section: Tbp-related Factorsmentioning

confidence: 99%

“…[61][62][63]65 Mouse TRF2 is essential for spermiogenesis. [79][80][81] It was recently demonstrated that TRF2 works in concert with the tissue-specific TAF7L, which is associated with testis-specific promoters, to regulate a subset of postmeiotic genes directing spermiogenesis. 82 Notably, Drosophila TRF2 has been shown to be involved in differentiation of germ cells of both male and female.…”

Section: Tbp-related Factorsmentioning

confidence: 99%

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TRF2: TRansForming the view of general transcription factors

et al. 2015

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These authors contributed equally to this work.Keywords: core promoter elements/motifs, DPE, embryonic development, histone gene cluster, RNAP II transcription, ribosomal protein genes, spermiogenesis, TBP-related factors, TRF2, TCT Transcriptional regulation is pivotal for development and differentiation of organisms. Transcription of eukaryotic protein-coding genes by RNA polymerase II (RNAP II) initiates at the core promoter. Core promoters, which encompass the transcription start site, may contain functional core promoter elements, such as the TATA box, initiator, TCT and downstream core promoter element. TRF2 (TATA-box-binding protein-related factor 2) does not bind TATA box-containing promoters. Rather, it is recruited to core promoters via sequences other than the TATA box. We review the recent findings implicating TRF2 as a basal transcription factor in the regulation of diverse biological processes and specialized transcriptional programs.

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Section: Tbp-related Factorsmentioning

confidence: 99%

Section: Tbp-related Factorsmentioning

confidence: 99%

TRF2: TRansForming the view of general transcription factors

et al. 2015

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“…Although other components of the RNA Polymerase II basal transcription apparatus impact meiosis (Zhang et al 2001;Hiller et al 2004), TAF4b is required for mitosis specifically in gonocytes and spermatogonia. TAF4b appears to be essential for germ cell stem cell differentiation and proliferation, which are required for the maintenance of fertility in adult males.…”

Section: Genes and Development 799mentioning

confidence: 99%

“…Control of spermatogenesis requires specialized transcriptional machinery in multiple species including Drosophila, Xenopus, mouse, and human (Martianov et al 2001;Zhang et al 2001;Wang and Page 2002;Han et al 2003;Hiller et al 2004). These alternative general transcription factors may be direct targets of testis-specific factors or function in the initiation of expression-specific genes in the testis that are expressed from alternative promoters (Sassone-Corsi 2002).…”

Section: Genes and Development 799mentioning

confidence: 99%

“…TRF2/TLF, a TBP-related factor, acts as a surrogate transcription factor for TBP in pachytene spermatocytes and post-meiotic spermatids (Martianov et al 2002). Loss of Trf2/Tlf causes infertility in mice (Zhang et al 2001). ALF (TFIIA ) is a germ cell-specific isoform of TFIIA, another component of the basal RNA Polymerase II machinery, that can substitute for TFIIA at specific promoters (Ozer et al 2000).…”

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Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID

Falender¹,

Freiman²,

Geles³

et al. 2005

Genes Dev.

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The establishment and maintenance of spermatogenesis in mammals requires specialized networks of gene expression programs in the testis. The gonad-specific TAF4b component of TFIID (formerly TAF II 105) is a transcriptional regulator enriched in the mouse testis. Herein we show that TAF4b is required for maintenance of spermatogenesis in the mouse. While young Taf4b-null males are initially fertile, Taf4b-null males become infertile by 3 mo of age and eventually exhibit seminiferous tubules devoid of germ cells. At birth, testes of Taf4b-null males appear histologically normal; however, at post-natal day 3 gonocyte proliferation is impaired and expression of spermatogonial stem cell markers c-Ret, Plzf, and Stra8 is reduced. Together, these data indicate that TAF4b is required for the precise expression of gene products essential for germ cell proliferation and suggest that TAF4b may be required for the regulation of spermatogonial stem cell specification and proliferation that is obligatory for normal spermatogenic maintenance in the adult. Spermatogenesis is a complex process requiring the specialized function of multiple cell types including somatic and germ cells that collectively results in the continuous production of functional sperm in adult males. The unlimited production of male gametes is largely accomplished through the ability of spermatogonial stem cells to self-renew in the adult testis. These complex and multifaceted events are dependent on appropriate expression and action of specific genes at multiple stages of germ cell and testicular development (Matzuk and Lamb 2002;McLaren 2003). The precise temporal and spatial expression of specific transcription factors is also essential for proper execution of spermatogenesis (SassoneCorsi 1997). Emerging evidence now suggests that in addition to gonad-specific transcription factors, specialized components of the basal RNA Polymerase II machinery are also critical for the execution of gonad-specific programs of gene expression (Hochheimer and Tjian 2003).The TFIID complex is a core RNA polymerase complex that contains the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs) that function in core promoter recognition and activator-dependent RNA Polymerase II recruitment (Verrijzer and Tjian 1996). While most TFIID subunits are expressed and function broadly in most cell types, there are selective TFIID subunits that apparently have evolved to function in the specification of gonadal-specific programs of gene expression. In the mouse, TAF4b is a component of TFIID that is highly enriched in gonadal tissues and is required for ovarian follicle development (Freiman et al. 2001). TAF4b is similar in structure to its broadly expressed paralog TAF4 (TAF II 130). While TAF4 and TAF4b display overlapping expression patterns in certain cell types, TAF4b is essential for regulating the selective expression of ovarian-specific gene expression patterns required for female fertility (Freiman et al. 2001).Several other members of the basal transcription mac...

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Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion

Shima

Aigaki

Nojima

et al. 2007

Arch Insect Biochem Physiol

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TATA-box-binding protein (TBP)-related factor (Trf)2 is a member of the family of TBP-related factors present in metazoan organisms. In Drosophila, Trf2 immunoprecipitates with the nucleosome remodeling factor (NURF) chromatin remodeling complex and the DNA replication element (DRE)-binding factor DREF. When it forms a complex with DREF, Trf2 activates transcription from the DRE-binding sites of the proliferating cell nuclear antigen (PCNA) gene. Despite these observations at the molecular level, no mutations in the trf2 locus have been found in Drosophila. Here, we identify two P-element insertion alleles, PL28 and GS7403, as hypomorphic mutants with a decreased expression level of trf2. Pupae of these mutant alleles show failure in anterior spiracle eversion, a hallmark of mutations in the loci associated with ecdysteroid signaling.

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Spermiogenesis Deficiency in Mice Lacking the Trf2 Gene

Cited by 189 publications

References 27 publications

TRF2: TRansForming the view of general transcription factors

TRF2: TRansForming the view of general transcription factors

Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID

Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion

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