Sphingopyxis microcysteis sp. nov., a novel bioactive exopolysaccharides-bearing Sphingomonadaceae isolated from the Microcystis phycosphere

Li, Guixian; Ge, Yaming; Iqbal, Nurhezreen Md; Qiao, Yingjie; Cui, Zhendong

doi:10.1007/s10482-021-01563-1

Cited by 13 publications

(9 citation statements)

References 36 publications

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“…The genome sequences of selected type strains within the family Dermatophilaceae were available until June 2021, and downloaded from GenBank genome database ( , accessed on 10 June 2021). The up-to-date bacterial core gene set (UBCG) was used to construct a phylogenetic tree using the genomes of strain NJES-13 and other reference type strains [ 21 , 48 ]. DNA G+C content of strain NJES-13 was calculated based on the genome sequence.…”

Section: Methodsmentioning

confidence: 99%

“…However, they were also not found in the type strain of Mobilicoccus pelagius. Based on the bioflocculanting activity assay of EPS of strain NJES-13, it is indicated that strain NJES-13 dwelling in the gut microenvironment of antarctic emperor penguin might act as a novel fresh candidate with great production potential for natural promising bioflocculants [21][22][23]. Additionally, ultrastructure observation of the cells of strain NJES-13 by transmission electron microscopy also clearly showed the presence of polyhydroxyalkanoates (PHA) granules inside the cells (Figure 1).…”

Section: Biosynthetic Genes Responsible For Active Metabolitesmentioning

confidence: 99%

“…ncbi.nlm.nih.gov/genome, accessed on 10 June 2021). The up-to-date bacterial core gene set (UBCG) was used to construct a phylogenetic tree using the genomes of strain NJES-13 and other reference type strains [21,48]. DNA G+C content of strain NJES-13 was calculated based on the genome sequence.…”

Section: Phylogenomic Analysismentioning

confidence: 99%

“…The prepared EPS were dissolved in distilled water for further bioflocculanting activity assay [23]. Briefly, the measurements using the kaolin clay suspension flocculation assay calculated as flocculation rate were used and performed in a 96-well microplate with a modified high-throughput manner in at least triplicate [20][21][22]. All the results are expressed as the means ± SD.…”

Section: Bacterial Eps Bioflocculanting Activity Assaymentioning

confidence: 99%

“…Indeed, microbial EPS derived from the extremophiles have shown diverse biotechnological promise, ranging from pharmaceutical industries for their immuno-modulatory and antiviral effects, and bone regeneration and cicatrizing capacity, to food industries for their peculiar gelling and thickening properties [13,18,19]. Moreover, some EPS are employed as novel microbial biosurfactants which can outcompete the traditional chemical flocculants due to their non-toxicity and extraordinary biodegradability characteristics [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Phylogenetic and Gephyromycin Metabolites Characterization of New Exopolysaccharides-Bearing Antarctic Actinobacterium from Feces of Emperor Penguin

2021

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A new versatile actinobacterium designated as strain NJES-13 was isolated from the feces of the Antarctic emperor penguin. This new isolate was found to produce two active gephyromycin analogues and bioflocculanting exopolysaccharides (EPS) metabolites. Phylogenetic analysis based on pairwise comparison of 16S rRNA gene sequences showed that strain NJES-13 was closely related to Mobilicoccus pelagius Aji5-31T with a gene similarity of 95.9%, which was lower than the threshold value (98.65%) for novel species delineation. Additional phylogenomic calculations of the average nucleotide identity (ANI, 75.9–79.1%), average amino acid identity (AAI, 52.4–66.9%) and digital DNA–DNA hybridization (dDDH, 18.6–21.9%), along with the constructed phylogenomic tree based on the up-to-date bacterial core gene (UBCG) set from the bacterial genomes, unequivocally separated strain NJES-13 from its close relatives within the family Dermatophilaceae. Hence, it clearly indicated that strain NJES-13 represented a putative new actinobacterial species isolated from the gut microbiota of mammals inhabiting the Antarctic. The obtained complete genome of strain NJES-13 consisted of a circular 3.45 Mb chromosome with a DNA G+C content of 67.0 mol%. Furthering genome mining of strain NJES-13 showed the presence of five biosynthetic gene clusters (BGCs) including one type III PKS responsible for the biosynthesis of the core of gephyromycins, and a series of genes encoding for bacterial EPS biosynthesis. Thus, based on the combined phylogenetic and active metabolites characterization presented in this study, we confidently conclude that strain NJES-13 is a novel, fresh actinobacterial candidate to produce active gephyromycins and microbial bioflocculanting EPS, with potential pharmaceutical, environmental and biotechnological implications.

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Section: Methodsmentioning

confidence: 99%

Section: Biosynthetic Genes Responsible For Active Metabolitesmentioning

confidence: 99%

Section: Phylogenomic Analysismentioning

confidence: 99%

Section: Bacterial Eps Bioflocculanting Activity Assaymentioning

confidence: 99%