Spider Chitin. The biomimetic potential and applications of Caribena versicolor tubular chitin

Machałowski, Tomasz; Wysokowski, Marcin; Żółtowska-Aksamitowska, Sonia; Bechmann, Nicole; Binnewerg, Björn; Schubert, Mario; Guan, Kaomei; Bornstein, Stefan R.; Czaczyk, Katarzyna; Pokrovsky, Oleg S.; Kraft, Michael; Bertau, Martin; Schimpf, Christian; Rafaja, David; Tsurkan, Mikhail V.; Galli, Roberta; Meißner, Heike; Petrenko, Iaroslav; Fursov, Andriy; Voronkina, Alona; Figlerowicz, Marek; Joseph, Yvonne; Jesionowski, Teofil; Ehrlich, Hermann

doi:10.1016/j.carbpol.2019.115301

Cited by 33 publications

(20 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, some maxima in the pattern could not be identified unambiguously. A large hump at 2θ ≈ 20° most likely corresponds to the α-chitin 110 reflection [4] since chitin belongs to the scaffold as was also identified by ATR-FTIR. A second contribution to this maximum can originate from amorphous components in the samples.…”

Section: Resultsmentioning

confidence: 63%

“…In recent decades, the synthesis and application of 3D biopolymer-based scaffolds represent one of the new trends in environmental science and technology. Owing to their ability to mimic the patterns of natural structures, excellent biocompatibility, high biodegradability and non-toxicity 3D scaffolds of natural origin find increasing applications in medicine, biotechnology and various interdisciplinary fields including tissue engineering, biomimetics, biocatalysis, adsorption techniques and wastewater treatment [1][2][3][4][5][6][7][8][9][10]. Highly versatile and promising biopolymers such as cellulose, chitin, collagen and their derivatives are more and more frequently used in modern technology [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Chitin is one of the most abundant polysaccharides of natural origin, obtained mainly from crustaceans, although it can be found in representatives of diatoms, sponges, mollusks, tubeworms, insects and arachnids [17][18][19][20][21][22]. This biopolymer is composed of β- (1,4)-N-acetyl-dglucosamine units and plays a crucial role in the formation of both soft and mineralized skeletal structures in invertebrates requiring rigidity and mechanical strength [23,24]. Usually, chitin as a source of industrially produced chitosan is isolated by two main types of extraction process: chemical and biological methods.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

Self Cite

View full text Add to dashboard Cite

Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering, regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated marine demosponge Aplysina aerophoba which consists of three main steps: (1) decellularization, (2) decalcification and (3) main deproteinization along with desilicification and depigmentation. For the first time, the obtained electrochemically isolated 3D chitinous scaffolds have been further biomineralized ex vivo using hemolymph of Cornu aspersum edible snail aimed to generate calcium carbonates-based layered biomimetic scaffolds. The analysis of prior to, during and post-electrochemical isolation samples as well as samples treated with molluscan hemolymph was conducted employing analytical techniques such as SEM, XRD, ATR-FTIR and Raman spectroscopy. Finally, the use of described method for chitin isolation combined with biomineralization ex vivo resulted in the formation of crystalline (calcite) calcium carbonate-based deposits on the surface of chitinous scaffolds, which could serve as promising biomaterials for the wide range of biomedical, environmental and biomimetic applications.

show abstract

Section: Resultsmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chitosan is a linear amino polysaccharide derived by deacetylation of chitin [10]. Chitin is the second most abundant natural polymer on the earth, a typical constituent of the cell walls of fungi; the exoskeletons of insects and crustaceans; and radula of mollusks [11][12][13]. Chitosan exhibits great properties, such as edibility, biodegradability, and biocompatibility [14].…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nanocrystals to Improve Stability and Functional Properties of Emulsified Film Based on Chitosan Nanoparticles and Beeswax

et al. 2019

View full text Add to dashboard Cite

The framework of this work was to develop an emulsion-based edible film based on a chitosan nanoparticle matrix with cellulose nanocrystals (CNCs) as a stabilizer and reinforcement filler. The chitosan nanoparticles were synthesized based on ionic cross-linking with sodium tripolyphosphate and glycerol as a plasticizer. The emulsified film was prepared through a combination system of Pickering emulsification and water evaporation. The oil-in-water emulsion was prepared by dispersing beeswax into an aqueous colloidal suspension of chitosan nanoparticles using high-speed homogenizer at room temperature. Various properties were characterized, including surface morphology, stability, water vapor barrier, mechanical properties, compatibility, and thermal behaviour. Experimental results established that CNCs and glycerol improve the homogeneity and stability of the beeswax dispersed droplets in the emulsion system which promotes the water-resistant properties but deteriorates the film strength at the same time. When incorporating 2.5% w/w CNCs, the tensile strength of the composite film reached the maximum value, 74.9 MPa, which was 32.5% higher than that of the pure chitosan film, while the optimum one was at 62.5 MPa, and was obtained by the addition of 25% w/w beeswax. All film characterizations demonstrated that the interaction between CNCs and chitosan molecules improved their physical and thermal properties.

show abstract

“…Furthermore, verongiid sponges can be successfully cultivated under marine farming conditions [63][64][65][66][67] and possess the unique ability to regenerate their skeletal tissues up to 12 cm per year [62].Mar. Drugs 2020, 18, x 3 of 27 corals [57], insects, spiders and crustaceans [58][59][60][61], is the size of sponges: they can reach up to 2 meters in diameter (i.e., the elephant ear sponge Ianthella basta (Pallas, 1766)) [27,62], or up to 1.5 meter length, as with the Caribbean stove-pipe sponge Aplysina archeri (Higgin, 1875) used in this study (Figure 1). Furthermore, verongiid sponges can be successfully cultivated under marine farming conditions [63][64][65][66][67] and possess the unique ability to regenerate their skeletal tissues up to 12 cm per year [62].…”

mentioning

confidence: 99%

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

et al. 2020

Self Cite

View full text Add to dashboard Cite

Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO3 amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure.

show abstract

Spider Chitin. The biomimetic potential and applications of Caribena versicolor tubular chitin

Cited by 33 publications

References 31 publications

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

Cellulose Nanocrystals to Improve Stability and Functional Properties of Emulsified Film Based on Chitosan Nanoparticles and Beeswax

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

Contact Info

Product

Resources

About