Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Jagadish, S. V. Krishna; Raveendran, M.; Rang, Zhongwen; Malo, Richard; Heuer, Sigrid; Bennett, John; Craufurd, Peter

doi:10.1007/s12284-011-9059-x

Cited by 85 publications

(74 citation statements)

References 41 publications

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“…They were grown in triplicate during the 2013 dry season at IRRI using a glasshouse maintained with ambient light, 29°C/21°C d/n temperature, and 75% to 85% relative humidity. Soil preparation, fertilizer regime, pesticide application, and glasshouse maintenance were as previously described (Jagadish et al, 2011).…”

Section: Transcriptome and Gene Regulatory Network Analysesmentioning

confidence: 99%

Systems Genetics Identifies a Novel Regulatory Domain of Amylose Synthesis

et al. 2016

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A deeper understanding of the regulation of starch biosynthesis in rice (Oryza sativa) endosperm is crucial in tailoring digestibility without sacrificing grain quality. In this study, significant association peaks on chromosomes 6 and 7 were identified through a genomewide association study (GWAS) of debranched starch structure from grains of a 320 indica rice diversity panel using genotyping data from the high-density rice array. A systems genetics approach that interrelates starch structure data from GWAS to functional pathways from a gene regulatory network identified known genes with high correlation to the proportion of amylose and amylopectin. An SNP in the promoter region of Granule Bound Starch Synthase I was identified along with seven other SNPs to form haplotypes that discriminate samples into different phenotypic ranges of amylose. A GWAS peak on chromosome 7 between LOC_Os07g11020 and LOC_Os07g11520 indexed by a nonsynonymous SNP mutation on exon 5 of a bHLH transcription factor was found to elevate the proportion of amylose at the expense of reduced short-chain amylopectin. Linking starch structure with starch digestibility by determining the kinetics of cooked grain amylolysis of selected haplotypes revealed strong association of starch structure with estimated digestibility kinetics. Combining all results from grain quality genomics, systems genetics, and digestibility phenotyping, we propose target haplotypes for fine-tuning starch structure in rice through marker-assisted breeding that can be used to alter the digestibility of rice grain, thus offering rice consumers a new diet-based intervention to mitigate the impact of nutrition-related noncommunicable diseases.

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Section: Transcriptome and Gene Regulatory Network Analysesmentioning

confidence: 99%

Systems Genetics Identifies a Novel Regulatory Domain of Amylose Synthesis

et al. 2016

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“…Three rice cultivars, rice 'IR64' (susceptible to water deficit stress), rice 'Apo' (aerobic/water deficit tolerant), and rice 'N22' (water deficit and high-temperature tolerant), were chosen for our study based on previous reports (Liu et al, 2006;Jagadish et al, 2011;Rang et al, 2011;Venuprasad et al, 2012). The two wheat cultivars selected were wheat 'SeriM82,' which is moderately susceptible (Pfeiffer, 1988) to tolerant of water-limited conditions (Villareal et al, 1995), and wheat 'Weebill4,' a highly drought-tolerant check cultivar Praba et al, 2009).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Does Morphological and Anatomical Plasticity during the Vegetative Stage Make Wheat More Tolerant of Water Deficit Stress Than Rice?

et al. 2015

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District of Columbia 20005 (P.S.B.)Water scarcity and the increasing severity of water deficit stress are major challenges to sustaining irrigated rice (Oryza sativa) production. Despite the technologies developed to reduce the water requirement, rice growth is seriously constrained under water deficit stress compared with other dryland cereals such as wheat (Triticum aestivum). We exposed rice cultivars with contrasting responses to water deficit stress and wheat cultivars well adapted to water-limited conditions to the same moisture stress during vegetative growth to unravel the whole-plant (shoot and root morphology) and organ/tissue (root anatomy) responses. Wheat cultivars followed a water-conserving strategy by reducing specific leaf area and developing thicker roots and moderate tillering. In contrast, rice 'IR64' and 'Apo' adopted a rapid water acquisition strategy through thinner roots under water deficit stress. Root diameter, stele and xylem diameter, and xylem number were more responsive and varied with different positions along the nodal root under water deficit stress in wheat, whereas they were relatively conserved in rice cultivars. Increased metaxylem diameter and lower metaxylem number near the root tips and exactly the opposite phenomena at the rootshoot junction facilitated the efficient use of available soil moisture in wheat. Tolerant rice 'Nagina 22' had an advantage in root morphological and anatomical attributes over cultivars IR64 and Apo but lacked plasticity, unlike wheat cultivars exposed to water deficit stress. The key traits determining the adaptation of wheat to dryland conditions have been summarized and discussed.Among cereals, rice (Oryza sativa) and wheat (Triticum aestivum) are the most important staple food crops, and they belong to the family Poaceae. These two cereals share a common ancestor and diverged about 65 million years ago (Sorrells et al., 2003). Rice eventually developed strong adaptation potential for fully flooded conditions across tropical to temperate environments, while wheat became well adapted to aerobic conditions mostly restricted to temperate environments. Rice, with a semiaquatic behavior, consumes about 30% of the total fresh water available for agricultural crops worldwide, which equates to a 2-to 3-fold higher consumption than other cereals such as wheat and maize (Zea mays; Peng et al., 2006). Despite a significantly lower water requirement, the potential yield of wheat in a favorable environment (9 tons ha 21 ) is comparable with the yield of fully flooded rice (9 tons ha 21 ) in the dry season at the International Rice Research Institute (IRRI; Fischer and Edmeades, 2010). Hence, rice records very low water productivity compared with wheat and other dryland cereals. Because of growing concerns about water scarcity and the increased frequency and magnitude of water deficit stress events under current and future climates, increasing or even sustaining rice yield under fully flooded conditions is highly challenging. To minimize the total water requ...

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“…A detailed description of the greenhouse and growth chamber conditions, including technical details, has been given elsewhere (Jagadish et al 2010a(Jagadish et al , 2011. Briefly, the main tillers of the plants were tagged and regularly monitored for the marker distance.…”

Section: Case Study -High-temperature Stressmentioning

confidence: 99%

“…The probability of these combined stresses damaging rice crops in the major ricegrowing regions in Southern and South-east Asia has been recently mapped (Wassmann et al 2009). The rice scientific community has intensified efforts to develop rice varieties capable of withstanding these adverse conditions (heat stress, Yoshida et al 1981;Jagadish et al 2010aJagadish et al , 2010bJagadish et al , 2011drought stress, Bernier et al 2007;Kumar et al 2008;Venuprasad et al 2008; cold stress, Andaya and Mackill 2003;Ji et al 2011) to sustain rice production under future adverse climates. Rice is extremely sensitive to these stresses, in particular, during the reproductive -gametogenesis and flowering stages -and exposure can result in increased spikelet sterility, which in turn, reduces grain yield.…”

Section: Introductionmentioning

confidence: 99%

A phenotypic marker for quantifying heat stress impact during microsporogenesis in rice (Oryza sativa L.)

Jagadish

Craufurd

Shi

et al. 2014

Functional Plant Biol.

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Abstract. Gametogenesis in rice (Oryza sativa L.), and particularly male gametogenesis, is a critical developmental stage affected by different abiotic stresses. Research on this stage is limited, as flowering stage has been the major focus for research to date. Our main objective was to identify a phenotypic marker for male gametogenesis and the duration of exposure needed to quantify the impact of heat stress at this stage. Spikelet size coinciding with microsporogenesis was identified using parafilm sectioning, and the panicle (spikelet) growth rate was established. The environmental stability of the marker was ascertained with different nitrogen (75 and 125 kg ha -1 ) and night temperature (22 C and 28 C) combinations under field conditions. A distance of -8 to -9 cm between the collar of the last fully opened leaf and the flag leaf collar, which was yet to emerge was identified as the environmentally stable phenotypic marker. Heat stress (38 C) imposed using the identified marker induced 8-63% spikelet sterility across seven genetically diverse rice genotypes. Identifying the right stage based on the marker information and imposing 6 consecutive days of heat stress ensures that >95% of the spikelets in a panicle are stressed spanning across the entire microsporogenesis stage.

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Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Cited by 85 publications

References 41 publications

Systems Genetics Identifies a Novel Regulatory Domain of Amylose Synthesis

Systems Genetics Identifies a Novel Regulatory Domain of Amylose Synthesis

Does Morphological and Anatomical Plasticity during the Vegetative Stage Make Wheat More Tolerant of Water Deficit Stress Than Rice?

A phenotypic marker for quantifying heat stress impact during microsporogenesis in rice (Oryza sativa L.)

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