Spikelet-specific variation in ethylene production and constitutive expression of ethylene receptors and signal transducers during grain filling of compact- and lax-panicle rice (Oryza sativa) cultivars

Sekhar, Sudhanshu; Panda, Binay; Mohapatra, Trupti; Das, Kaushik; Shaw, Birendra Prasad; Kariali, Ekamber; Mohapatra, Pravat K.

doi:10.1016/j.jplph.2015.03.003

Cited by 61 publications

(99 citation statements)

References 64 publications

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“…At maturity, the control plant had higher carbohydrate content in the stem but lower in the grains (Figure 8) due to early senescence that reduced photosynthetic rate and leaf area compared to SPM treatment. Similar results were reported by Kuanar et al (2010), Ji et al (2012) and Sekhar et al (2015).…”

Section: Discussionsupporting

confidence: 91%

“…In rice, endosperm SUS activity is considered as a potential indicator of high grain yield (Counce and Gravois 2006). Activity of the enzyme is low in the inferior spikelets compared to the superior spikelets (Mohapatra et al 2011), and it is positively linked to the strength of the developing grain to accept sucrose (Yang et al 2003) The increase in SUS enzyme activity in the present study corroborated with increased expression of SUS gene which was in accordance with the findings of Cai et al (2004), Liu et al (2008) and Sekhar et al (2015). Rice is formed mainly through the synthesis and accumulation of starch and protein.…”

Section: Discussionsupporting

confidence: 91%

“…Assimilation rates in photosynthetic leaves increased due to enhanced photosynthetic metabolites and enzyme activity which directly improved grain productions (Lisar et al, 2012). In this study, SPM proved to be an effective growth enhancer as it increased the activity of SUS enzyme ( Figure 5) which translated into enhanced grain filling and improved yield attributes under water stress which was in agreement with other studies (Cai et al 2004;Liu et al 2008;Sekhar et al 2015).…”

Section: Discussionsupporting

confidence: 90%

“…On the other hand, the inadequate supply of assimilate in control plant consequently delayed sucrose and starch accumulation whereas sucrose availability for translocation to the grains was reduced. These is postulated to be the main inhibitor for the slow and poor grain filling of the inferior spikelets as reported by Mohapatra et al (2011), Dong et al (2012), Lemoine et al (2013) and Sekhar et al (2015), particularly due to water stress. The synthesis of sucrose and starch as well as SUS activity during grain filling were totally interrupted when water stress was imposed which was in agreement with other studies (Zhu et al 2011 andSekhar et al 2015).…”

Section: Discussionmentioning

confidence: 83%

“…Sucrose is actively loaded into the developing rice grains and subsequently converted into starch by a series of enzymatic steps (Mohapatra et al 2011). It has been reported that the low activities of starch synthesis-related enzymes, such as sucrose synthase enzyme (SUS), ADP-glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS), and starch branching enzyme (SBE), are closely associated with the inferior grain weight (Kato et al 2007;Panda et al 2009;Tang et al 2009;Sekhar et al 2015). Meanwhile, some of these enzymes have been shown to be differentially expressed at the transcript level in both inferior and superior caryopses during grain filling (Ishimaru et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of sucrose synthase and its association with grain filling in spermine-treated rice plant under water deficit

Berahim

Dorairaj

Saud

et al. 2019

Journal of Plant Interactions

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Spermine (SPM) was utilized to investigate its impact on the accumulation of sucrose synthase (SUS) and the role of SUS as a predictor of sink strength of rice under cyclic water stress. Treatments which consisted of control and SPM were arranged in a randomized complete block design. Biochemical analyses showed significantly higher contents of sucrose, starch and carbohydrate in SPM-treated panicles which pointed to increased loading of sucrose into the grains or conversion of sucrose into starch. Besides, the expression of SUS gene was up-regulated in both inferior and superior spikelets at twenty and three fold, respectively. Correspondingly, SUS enzyme showed an increase in its activity. The high expression of SUS3 during grain filling is linked to the increased capacity for starch synthesis. Grain weight and grain filling rate of SPM-treated spikelets improved due to their large sink capacity and increased number of spikelets per panicle.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation of sucrose synthase and its association with grain filling in spermine-treated rice plant under water deficit

Berahim

Dorairaj

Saud

et al. 2019

Journal of Plant Interactions

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Grain filling in rice vis‐a‐vis ethylene production in the spikelets

et al. 2023

Self Cite

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Rice being a staple food worldwide necessitates that its production be enhanced to meet the demands of the continuously increasing population. However, the productivity advantages associated with breeding programs have reached their maximum. The production plateau brought on is not due to a lack of genetic potential but rather is due to the inhibition of grain filling by ethylene produced in large quantities in the heavy‐panicle‐ and compact‐panicle‐type varieties developed thus far, as evidenced by the application of ethylene synthesis inhibitors and ethylene action blockers. The inhibition could be a result of the accumulation of HCN, a byproduct of ethylene biosynthesis. In addition, inhibition of grain filling by excessive amounts of ethylene may also be the result of enhanced synthesis/expression of rice starch regulator 1, an ethylene‐responsive element binding protein that inhibits expression of the genes encoding type I starch‐biosynthesizing enzymes. In the past decade, miRNAs have also been found to inhibit grain filling by targeting starch‐biosynthesizing enzymes and the transcription factors required for their expression. Hence, biotechnological interventions should be designed to (1) decrease ethylene production and CN− accumulation and (2) downregulate/knockdown the expression of miRNAs unfavorable to grain filling in a spikelet/seed‐specific manner to enhance rice production.

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Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

Wang

Gong

et al. 2020

Food and Energy Security

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Moderate soil drying (MD) applied during the mid‐to‐late grain filling stages can promote carbon reserve remobilization in straws (sheaths and stems) and increase the grain weight. The coordination between starch‐to‐sucrose transition in straws and sucrose‐to‐starch conversion in inferior grains is essential for carbon reserve remobilization during grain filling. Herein, to reveal the regulating mechanism of carbon reserve remobilization from source to sink, RNA‐seq was utilized to analyze the dynamic transcript profile in source and sink of rice under MD treatment during grain filling. The expression of amylase genes and amylase activity was enhanced by MD treatment in straws, which is consistent with the increased amylase activity. In inferior grains, it was starch synthesis genes that were upregulated by MD treatment. Furthermore, an elevated ABA was found in both straws and inferior grains, which was caused by an increased expression of NCED1 and downregulation of ABA8OX2 by MD treatment, respectively. Additionally, the expression of MYB30, a transcription factor (TF) that negatively regulates beta‐amylase genes, was reduced in straws by MD, resulting in an increased amylase activity. In contrast, an increased expression of NAC activated the expressions of starch synthesis gene in inferior grains under MD. Therefore, it is MYB30 and NAC that cooperates in source and sink, respectively, to promote carbon reserve remobilization in response to MD. Taken together, genes involved in carbon flow from source to sink are different between rice straws and inferior grains.

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Spikelet-specific variation in ethylene production and constitutive expression of ethylene receptors and signal transducers during grain filling of compact- and lax-panicle rice (Oryza sativa) cultivars

Cited by 61 publications

References 64 publications

Regulation of sucrose synthase and its association with grain filling in spermine-treated rice plant under water deficit

Regulation of sucrose synthase and its association with grain filling in spermine-treated rice plant under water deficit

Grain filling in rice vis‐a‐vis ethylene production in the spikelets

Expression profile of the carbon reserve remobilization from the source to sink in rice in response to soil drying during grain filling

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