Spiking venom with rVes v 5 improves sensitivity of IgE detection in patients with allergy to Vespula venom

Vos, B. J. P. R.; Köhler, Julian; Müller, Sabine; Stretz, E.; Ruëff, Franziska; Jakob, Thilo

doi:10.1016/j.jaci.2012.07.041

Cited by 88 publications

(81 citation statements)

References 9 publications

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“…Moreover, in a study of 308 subjects, ImmunoCAP revealed rates of sensitization (0.35 UA/ml) of 83.4% for conventional yellow jacket (Vespula vulgaris) venom, 44.2% for rVes v 1, 89.9% for rVes v 5, and 96.1% for either rVes v 1 or rVes v 5, or both. 5 In particular, in patients sensitive to yellow jacket venom, the diagnostic sensitivity of a combination of the currently available rVes v 5 and rVes v 1 has been reported to be as high as 92e96%, 2,5,6 which is similar to our results. Compared with our data, these reports of sensitization to Ves v 5 alone or to both Ves v 1 and Ves…”

Section: Dear Editorsupporting

confidence: 91%

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Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Hirata

Yoshida

Watanabe

et al. 2015

Allergology International

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Section: Dear Editorsupporting

confidence: 91%

“…A recent study reported that spiking conventional hornet venom with rVes v 5 improves the sensitivity of IgE detection in patients allergic to Vespula venom. 5 rVes v 5-spiked yellow jacket venom will provide a more reliable method for detecting hornet venom sensitization in patients with Hymenoptera allergy.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Hirata

Yoshida

Watanabe

et al. 2015

Allergology International

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“…This alteration could be due to the use of new venom allergen standards. There are reports that major allergens are missing or underrepresented in the hymenoptera venom standards, e. g., Ves v5 in the wasp venom extract [25] and Api m10 in the bee venom standard [26]. The importance of these insufficiently represented allergens was shown by Vos et al., who were able to increase sensitivity to an assay from 84.4 to 96.8% by spiking the extract with recombinant (r) Ves v5 [25].…”

Section: Discussionmentioning

confidence: 99%

Six years of INSTAND e. V. sIgE proficiency testing

et al. 2017

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BackgroundEven though allergies are an important health issue, wide manufacturer-dependent differences in the detected amounts of allergen-specific IgE (sIgE) have repeatedly been found. These discrepancies hinder diagnostics and research into clinically significant cutoff points for life-threatening symptoms.MethodsTo evaluate whether the reported differences have led to changes in diagnostic testing, we analyzed data from six years of round robin testing (RRT, also known as proficiency testing) at the Gesellschaft zur Förderung der Qualitätssicherung in medizinischen Laboratorien e.V. (Society for Promoting Quality Assurance in medical Laboratories) for the important allergen sources bee venom, wasp venom, and birch pollen. The results of the four main suppliers of in vitro diagnostic sIgE testing were compared in a pseudo-anonymized form using overlay images of box plot graphs for the semiquantitative data and allergen class results. Coefficients of variation (CV) were obtained to study the development of interlaboratory comparability.ResultsWe found that the large differences between the manufacturer collectives remained constant between January 2010 and April 2015 without any real improvement. The CVs were good for two of the four analyzed suppliers, one was marginal and one above the quality level of 20%.ConclusionThe numerous publications that have found discrepancies in the sIgE results of the different suppliers did not change the status quo within the last six years. Unfortunately, this is unlikely to change until there is a characterized standard material with known values of sIgE.

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“…In a sample of 308 patients with SR due to Vespula stings, Vos et al [30] found that only 83.4% of the patients had positive specific IgE, as measured by ImmunoCAP with Vespula venom. However, when molecular allergens such as Ves v 5 were used, as many as 96% of patients were sensitized, with the levels of specific IgE to Ves v 5 being much higher than the levels of specific IgE to Vespula.…”

Section: Systemic Reactions Due To Hymenoptera Stings With Negative Smentioning

confidence: 99%

Key Issues in Hymenoptera Venom Allergy: An Update

Alfaya

Soriano-Gomis

Mera

et al. 2017

J Investig Allergol Clin Immunol

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In this review, the Hymenoptera Allergy Committee of the SEAIC analyzes the most recent scientific literature addressing problems related to the diagnosis of hymenoptera allergy and to management of venom immunotherapy. Molecular diagnosis and molecular risk profiles are the key areas addressed. The appearance of new species of hymenoptera that are potentially allergenic in Spain and the associated diagnostic and therapeutic problems are also described. Finally, we analyze the issue of mast cell activation syndrome closely related to hymenoptera allergy, which has become a new diagnostic challenge for allergists given its high prevalence in patients with venom anaphylaxis.

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Spiking venom with rVes v 5 improves sensitivity of IgE detection in patients with allergy to Vespula venom

Cited by 88 publications

References 9 publications

Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Six years of INSTAND e. V. sIgE proficiency testing

Key Issues in Hymenoptera Venom Allergy: An Update

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