Spinal neurons in the zebrafish labeled with Fluoro-gold and wheat-germ agglutinin

Asselt, Els van; Graaf, F. de; Smit-Onel, M.J.; Raamsdonk, W. van

doi:10.1016/0306-4522(91)90320-n

Cited by 12 publications

(5 citation statements)

References 50 publications

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“…Various lectins have been used in the study of brain, kidney, and hematopoietic tissue of zebrafish. [31][32][33][34][35] Yet lectins have rarely been applied to cardiovascular studies of non-mammalian models including the zebrafish. In studies where lectin reactivity with fish heart tissue has been tested, little to no binding was found.…”

Section: Introductionmentioning

confidence: 99%

Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio (Devario aequipinnatus) and Zebrafish (Danio rerio) Hearts

Manalo

May

Quinn

et al. 2016

J Histochem Cytochem.

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SummaryLectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish (Danio rerio) and giant danio (Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins-wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutinin (tomato lectin)-in these hearts. Con A showed broad staining in the myocardium. WGA stained cardiac myocyte borders, with binding markedly stronger in the compact heart and bulbus. BS lectin, which stained giant danio coronaries, was used to measure vascular reconstruction during regeneration. However, BS lectin reacted poorly in zebrafish. RCA I stained the compact heart of both fish. Tomato lectin stained the giant danio, and while low reactivity was seen in the zebrafish ventricle, staining was observed in their transitional cardiac myocytes. In addition, we observed unique staining patterns in the developing zebrafish heart. Lectins' ability to reveal differential glycoconjugate expression in giant danio and zebrafish hearts suggests they can serve as simple but important tools in studies of developing, adult, and regenerating fish hearts. (J Histochem Cytochem 64:687-714, 2016)

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Section: Introductionmentioning

confidence: 99%

Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio (Devario aequipinnatus) and Zebrafish (Danio rerio) Hearts

Manalo

May

Quinn

et al. 2016

J Histochem Cytochem.

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“…Some of these synapses were present on very large neurons in the spinal cord ventrolateral to the central canal. Judging by the size and position of these neurons they were probably primary motor neurons (van Asselt et al, 1991). These observations suggest that at least some of the previous targets of descending axons are re-innervated.…”

Section: Descending Projectionsmentioning

confidence: 81%

Zebrafish as a Model System for Successful Spinal Cord Regeneration

Becker

2006

Model Organisms in Spinal Cord Regeneration

101

114

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OverviewAdult zebrafish, in contrast to mammals, are capable of functional regeneration after spinal cord injury. Regeneration studies in zebrafish benefit from the model status of this vertebrate for research on developmental biology. This model status has helped to make available molecular tools such as genetic manipulation, expression profiling and gene knock-down techniques. Behavioral recovery after spinal injury is clearly quantifiable in zebrafish using different tests. The anatomical basis for behavioral recovery is supported by a surprising heterogeneity in axon regrowth and gene expression in different neuronal populations. In addition, specific gene regulation in non-axotomized spinal neurons indicates plasticity of the spinal circuitry after injury, which may also contribute to behavioral recovery. Gaps in our understanding of the spinal network are being filled by several studies of the more simple spinal network found in larval zebrafish. Gene knock-down, interference with intracellular messenger systems and promoter analysis in neurons after lesion are all possible in vivo, demonstrating the experimental accessibility of the system. At the same time, environmental inhibitors of axon re-growth appear not to be prominent in fish. Thus, environmental conditions may not confound the study of neuronal reactions to axotomy. Zebrafish, therefore, offer the opportunity to perform in-vivo studies of the mechanisms that lead to successful recovery after spinal cord injury, from the molecular to the systems levels.

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“…The use of lectins, such as Maclura pomifera , peanut agglutinin, and wheat germ agglutinin, to mark specific developmental stages or processes ranging from oogenesis to chondrogenesis and neurogenesis is well established and provided the earliest indications that glycan expression is dynamic during zebrafish embryogenesis [55–57]. More recently, advances in chemical biology methods have enabled specific classes of glycans to be visualized in living zebrafish embryos.…”

Section: Visualizing Glycans In Zebrafishmentioning

confidence: 99%

“Casting” light on the role of glycosylation during embryonic development: Insights from zebrafish

Flanagan-Steet

Steet

2012

Glycoconj J

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Zebrafish (Danio rerio) remains a versatile model organism for the investigation of early development and organogenesis, and has emerged as a valuable platform for drug discovery and toxicity evaluation [1–6]. Harnessing the genetic power and experimental accessibility of this system, three decades of research have identified key genes and pathways that control the development of multiple organ systems and tissues, including the heart, kidney, and craniofacial cartilage, as well as the hematopoietic, vascular, and central and peripheral nervous systems [7–31]. In addition to their application in large mutagenic screens, zebrafish has been used to model a variety of diseases such as diabetes, polycystic kidney disease, muscular dystrophy and cancer [32–36]. As this work continues to intersect with cellular pathways and processes such as lipid metabolism, glycosylation and vesicle trafficking, investigators are often faced with the challenge of determining the degree to which these pathways are functionally conserved in zebrafish. While they share a high degree of genetic homology with mouse and human, the manner in which cellular pathways are regulated in zebrafish during early development, and the differences in the organ physiology, warrant consideration before functional studies can be effectively interpreted and compared with other vertebrate systems. This point is particularly relevant for glycosylation since an understanding of the glycan diversity and the mechanisms that control glycan biosynthesis during zebrafish embryogenesis (as in many organisms) is still developing. Nonetheless, a growing number of studies in zebrafish have begun to cast light on the functional roles of specific classes of glycans during organ and tissue development. While many of the initial efforts involved characterizing identified mutants in a number of glycosylation pathways, the use of reverse genetic approaches to directly model glycosylation-related disorders is now increasingly popular. In this review, the glycomics of zebrafish and the developmental expression of their glycans will be briefly summarized along with recent chemical biology approaches to visualize certain classes of glycans within developing embryos. Work regarding the role of protein-bound glycans and glycosaminoglycans (GAG) in zebrafish development and organogenesis will also be highlighted. Lastly, future opportunities and challenges in the expanding field of zebrafish glycobiology are discussed.

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Spinal neurons in the zebrafish labeled with Fluoro-gold and wheat-germ agglutinin

Cited by 12 publications

References 50 publications

Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio (Devario aequipinnatus) and Zebrafish (Danio rerio) Hearts

Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio (Devario aequipinnatus) and Zebrafish (Danio rerio) Hearts

Zebrafish as a Model System for Successful Spinal Cord Regeneration

“Casting” light on the role of glycosylation during embryonic development: Insights from zebrafish

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