Spiny-cheek crayfish Orconectes limosus carry a novel genotype of the crayfish plague pathogen Aphanomyces astaci

Kozubíková, Eva; Viljamaa-Dirks, Satu; Heinikainen, Sirpa; Petrusek, Adam

doi:10.1016/j.jip.2011.08.002

Cited by 74 publications

(75 citation statements)

References 17 publications

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“…All 6 ITS sequences obtained from isolates from 5 O. limosus from both Sites CO and BE, and 1 A. leptodactylus from BE, were invariant. They corresponded exactly to sequences from A. astaci strains of all 5 known genotype groups of the pathogen, including the one obtained from O. limosus (Kozubíková et al 2011a). The sequencing thus corroborated the results of the real-time PCR analysis.…”

Section: Molecular Detection Of Aphanomyces Astacimentioning

confidence: 56%

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Invasive crayfish and crayfish plague on the move: first detection of the plague agent Aphanomyces astaci in the Romanian Danube

Pârvulescu

Schrimpf²,

Kozubíková³

et al. 2012

Dis. Aquat. Org.

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Section: Molecular Detection Of Aphanomyces Astacimentioning

confidence: 56%

“…The PCR products were sequenced in the forward direction using the primer 42, and resulting sequences (GenBank accession numbers JN713915−JN713917) were aligned in Kozubíková et al 2011a). The alignment was then checked visually for the presence of differences among sequences.…”

Section: Molecular Diagnosticsmentioning

confidence: 99%

Invasive crayfish and crayfish plague on the move: first detection of the plague agent Aphanomyces astaci in the Romanian Danube

Pârvulescu

Schrimpf²,

Kozubíková³

et al. 2012

Dis. Aquat. Org.

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“…Five major RAPD genotypes of A. astaci have been identified (Huang et al 1994, Diéguez-Uribeondo et al 1995, Kozubiková et al 2011, with two of them causing annual crayfish plague epidemics in Finland (Vennerström et al 1998). Finland is among the last refuges of the Asgenotype, which has been identified as the genotype present in the first invasion of A. astaci to Europe .…”

Section: Introductionmentioning

confidence: 99%

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Makkonen

Jussila²,

Kortet³

et al. 2012

Dis. Aquat. Org.

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Crayfish plague epidemics (caused by Aphanomyces astaci) have been causing population collapses among native European crayfish stocks since the late 1800s. Recent indirect and direct evidence has shown that its virulence has been variable, with native European crayfish even acting as carriers. We tested the differences in A. astaci virulence under experimental conditions using both PsI-and As-genotypes with 3 Finnish noble crayfish Astacus astacus populations. We infected crayfish with adjusted quantities of A. astaci zoospores and monitored the symptoms and mortality of the crayfish. The PsI-genotype isolate caused rapid and total mortality among the tested populations, while the As-genotype isolates expressed more variable virulence. In some cases, mortality among the As-genotype-infected crayfish did not exceed the mortality level of the control group. All of the tested noble crayfish stocks showed lower mortality towards the As-genotype of A. astaci isolated from the River Kemijoki epidemic. We conclude that there are clear differences in virulence between different A. astaci genotypes and also differences in virulence within As-genotypes. Furthermore, we observed clear signs of increased resistance in different populations of noble crayfish towards some of the tested strains belonging to the Asgenotype of A. astaci.

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“…The situation has actually been further reinforced since the signal crayfish introductions have often been accompanied by the notably virulent PsI-genotype crayfish plague (Souty-Grosset et al, 2006;Jussila et al, 2013a). Furthermore, the more recently introduced A. astaci genotypes, namely PsI, PsII, Pc and Or (Kozubíková et al, 2011), are, if possible, an even greater threat to the native European crayfish than the originally introduced As-genotype, since unlike the As-genotype, they so far have not had to adapt to susceptible host species in Europe. The status of the alien species, in this case both the crayfish plague and its introduced North American host crayfish species, has been debated intensively (Gherardi and Holdich, 1999).…”

Section: Introductionmentioning

confidence: 99%

Aphanomyces astaciPsI-genotype isolates from different Finnish signal crayfish stocks show variation in their virulence but still kill fast

Jussila

Kokko

Kortet

et al. 2013

Knowl. Managt. Aquatic Ecosyst.

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Key-words:crayfish plague, noble crayfish, potent killer, Psorospermium haeckeliWe detected significant virulence differences among five tested (PsI-Puujärvi, PsI-Pyhäjärvi, PsI-Kukkia, PsI-Saimaa I and PsI-Saimaa II) PsI-genotype crayfish plague (Aphanomyces astaci) isolates against lake Mikitänjärvi noble crayfish population. The crayfish were inoculated with a dose of 300 m·L −1 A. astaci spores in ambient water under experimental conditions. Mortalities started from four to seven days after inoculation, depending on the PsI-genotype isolate. In all the experimentally infected groups it took no more than three days for all the crayfish to die after the first mortality. The PsI-Puujärvi isolate proved to be the most virulent strain, while PsI-Kukkia was the least virulent. The average day of death for these experimental groups was fifth and ninth day, respectively. We did not discover correlation between the average day of death and gender or level of additional Psorospermium haeckeli infestation. Our results show that there are, from the practical point of view, minor virulence differences among PsI-genotype A. astaci isolates, and that all the tested five isolates are highly virulent. The present results emphasize the necessity to prevent all further spread of highly virulent strains of A. astaci to aid and shelter successful conservations attempts of the native European crayfishes.

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Spiny-cheek crayfish Orconectes limosus carry a novel genotype of the crayfish plague pathogen Aphanomyces astaci

Cited by 74 publications

References 17 publications

Invasive crayfish and crayfish plague on the move: first detection of the plague agent Aphanomyces astaci in the Romanian Danube

Invasive crayfish and crayfish plague on the move: first detection of the plague agent Aphanomyces astaci in the Romanian Danube

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Aphanomyces astaciPsI-genotype isolates from different Finnish signal crayfish stocks show variation in their virulence but still kill fast

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