Spirafolide from bay leaf (Laurus nobilis) prevents dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells

Abstract

Reactive oxygen species (ROS) are important mediators in many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. This study tested the neuroprotective effects of spirafolide, a compound purified from the leaves of Laurus nobilis L. (Lauraceae), against dopamine (DA)-induced apoptosis in human neuroblastoma SH-SY5Y cells. Following a 24-h exposure of cells to DA (final conc., 0.6 mM), we observed a marked increase in apoptosis, increased generation of ROS and decreased cell viability. Pretreatment of the cells for 24 h with spirafolide (0.4, 2, and 10 μM) before exposure to DA notably increased cell survival (p < 0.01) and lowered intracellular ROS levels (p < 0.01). These results indicate that spirafolide has neuroprotective effects against DA toxicity. These effects may contribute to the treatment of neurodegenerative diseases.

Bay Leaf (Laurus Nobilis L.) Incense Improved Scopolamine-Induced Amnesic Rats by Restoring Cholinergic Dysfunction and Brain Antioxidant Status

“…Turkez et al [66] demonstrated the preventive role of L. nobilis leaf extract in alleviating aluminum phosphide-induced DNA and oxidative damages in vitro. Ham et al [67] reported that spirafolide from the bay leaf (L. nobilis) prevented dopamine-induced…”

“…Turkez et al [66] demonstrated the preventive role of L. nobilis leaf extract in alleviating aluminum phosphide-induced DNA and oxidative damages in vitro. Ham et al [67] reported that spirafolide from the bay leaf (L. nobilis) prevented dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells. BL exposure effectively restored the antioxidant defense mechanism by increasing the antioxidant levels of activity in the brain.…”

Downregulation of Reactive Oxygen Species in Apoptosis

“…Spirafolide is a compound purified from the leaves of Laurus nobilis L. It has been reported to decrease the ROS level, thereby inhibited dopamine-induced apoptosis in human neuroblastoma SH-SY5Y cells. 39 Fructose, when used as sole carbon source instead of glucose, reduced ROS and stabilized of cellular GSH pool as efficient as N-acetyl-cystein in the oxidative stress-induced apoptosis in liver parenchymal cells. 40 …”

Aluminium phosphide-induced genetic and oxidative damages in vitro: Attenuation by Laurus nobilis L. leaf extract

“…Recent reports were also exhibited the neuroprotective, cardioprotective and antidiabetic properties of LNE due to its antioxidant content. [2627] Dall’Acqua et al . [28] determined the phytochemical composition L. nobilis leaves and found ten different flavonoid O-glycosides, one flavonoid C-glycoside, catechin, and cinnamtannin B1.…”