Spleen autotransplantation. Morphological and functional follow‐up after spleen autotransplantation in mice: A research summary

Abstract

In 1986, we started the research on spleen surgery aimed at saving the splenic mass after its traumatic injury, with elaboration of special resection and autotransplantation techniques. The researches started on mongrel dogs and were continued on inbred mice and beagle dogs with complex histological, imaging, and laboratory investigations, following-up the function and the regeneration of autotransplanted spleen chips. Performing research on mice provided more immunological methods, such as lymphocyte subsets, immunoglobulin levels, and monitoring the phagocytic functions. Researches showed evidence also on the presence of apoptosis, furthermore, stem cell studies on regeneration and functional restoration of the spleen chips are in progress. Our results contributed to two multidisciplinary guidelines in Hungary: (1) One of them is under preparation and underlines the importance of spleen saving methods after traumatic splenic injury; (2) The second guideline shows that hemorheological changes can be early indicators of the increased sensitivity to postsplenectomy infections.

Long-term following-up of viability of spleen autotransplants in the Beagle canine model

“…Although not all of the spleen chips were found in the autotransplantation with 5 spleen chips group, the previous investigation of our Department did not find correlation between the number of the implanted spleen chips and their viability 14,19 .…”

“…This difficulty exists because there's no elaborated diagnostic protocol for these aims 12,[17][18][19][20] .…”

Murine Spleen Tissue Regeneration from Neonatal Spleen Capsule Requires Lymphotoxin Priming of Stromal Cells

“…Although the risk of overwhelming postsplenectomy infection is relatively low, progression to fulminant sepsis can occur rapidly and mortality rates are estimated to reach 50-70% (2,3). To retain spleen function following splenectomy, a method for whole-spleen tissue fragmentation into thin slices and reimplantation into omental pouches has been studied in animal models and applied to human surgical procedures (4,5).…”

Assessing the spleen autotransplant effect, which common test is the choice?

“…The mean age of the patients was 20.7 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) years. Abdominal blunt trauma was the main reason for splenic rupture in the participants.…”