Anastomotic leakage following colorectal resection is reported to have a significant mortality and morbidity. Various surgical technologies have been developed to improve the outcome of colonic anastomotic, including biomaterial protective agent which has limitations in expensive price and rejection risk. The free peritoneal graft contains growth factors, antimicrobial agent and progenitor or stem cell nature by Mesothelial-Mesenchymal transition mechanism for wound healing process. Examining the healing of colonic anastomotic treated with free peritoneal graft in the presence of intraperitoneal infection by measuring the bursting pressure of the colonic tissue after surgery. We conducted an experimental study using 30 fecal peritonitis-induced New Zealand White Rabbit in 2 sample groups. Both of them have half circumferential diameter colonic resection treated with primary anastomotic using 5/0 silk braided suture material. In the treatment group, free peritoneal auto-graft was harvested and applicated. Bursting pressure was measured on the colonic tissue treated with anastomosis on day-7. The changes in the rabbits’ body weight before and after surgery which describe the nutritional status did not significantly affect the bursting pressure value (P = 0.745).