Spondias mombin Seed Oil Compounds Identification by Raman Spectroscopy and NMR

Abstract

Spondias mombin L. has been used in traditional medicine to treat some cases such as infections and inflammations. Some researchers have reported that its biological components, such as carotenoids, carotenes, and phenols, have been characterized primarily by HPLC analysis. Here, we report on the characterization of Spondias mombin L. seed oil by Raman spectroscopy, and the profile identification of fatty acids by 1H-NMR and 13C-NMR spectroscopy. The oil was extracted from different weight volumes of seeds using organic solvent, and each batch was characterized. The analysis of the fatty acid profile by NMR indicated that the seed oil is highly unsaturated (monounsaturated: 29.4% and polyunsaturated: 43.5%). Molecular Raman vibrations at 1006, 1158 and 1523 cm−1 showed the presence of carotenoids, which in turn performed an antioxidant activity. This was demonstrated by a 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) method. The cell viability in colon cancer cells was promoted in the presence of the oil. The compounds identified in this study from seed oil could be an interesting proposal for food or pharmaceutical applications.

Flours from Spondias mombin and Spondias tuberosa seeds: Physicochemical characterization, technological properties, and antioxidant, antibacterial, and antidiabetic activities

“…López‐Camacho et al. (2021) evaluated the yellow mombin seed oil describing a concentration‐dependent antioxidant activity, with a maximum ABTS radical inhibition of 45%. Another study with Brazil plum residues showed similar elimination of ABTS and DPPH to that reported in the present study (Xavier et al., 2022).…”

Characterization of the Nonpolar and Polar Extractable Components of Glanded Cottonseed for Its Valorization

“…While both walnut and bluebell oil samples showed a higher percentage of unsaturated fatty acids than Gd cottonseed, walnut was dominated by PUFAs, and bluebell was dominated by MUFAs. Similarly, Barison et al [ 60 ] applied these 1 H NMR spectral characteristics to develop a simple method for the determination of linolenic, linoleic, oleic, and saturated fatty acids. They found the ratio changes of these fatty acid compositions in oil samples of rice, sunflower, corn, canola, olive, and soybean.…”

“…They found the ratio changes of these fatty acid compositions in oil samples of rice, sunflower, corn, canola, olive, and soybean. However, it is impossible to quantitatively compare those data with those in Table 2 not only due to the difference in the specific fatty types but also as no tabulated quantitative values were presented in [60]. a : Averages calculated per the five-year data in [59].…”

Chemical Composition and Anti-Microbial Activity of Hog Plum (Spondias mombin L.) Peel Oil Extracted from Different Regions of Tropical Climates

“…Essential oils from citrus fruits are a group of natural flavors and fragrances which are popularly used in the food and pharmaceutical industries, daily chemical products, and in the health care field [8][9][10][11][12]. The products of medicinal plants like EOs and their antimicrobial properties have been empirically recognized for centuries, but recently the antimicrobial properties have been studied and confirmed scientifically [13][14][15]. These EOs effectively control the growth of different microorganisms like fungus, yeasts, bacteria, etc., which has been reported in several studies [16][17][18][19][20][21].…”