Spontaneous assembly of DNA–amorphous calcium phosphate nanocomposite spheres for surface-mediated gene transfer

In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering

“…ACP nanoparticles are known to be fabricated easily via homogeneous CaP nucleation (amorphous phase nucleation) in highly supersaturated labile CaP solutions. In our previous studies, a dense polymer substrate was coated with ACP nanoparticles utilizing a labile CaP solution [36,37]. These studies reported that ACP nanoparticles formed promptly in the CaP solution upon mixing source Ca and P solutions, and deposited onto the substrate via gravity sedimentation.…”

Improved gene transfer efficiency of a DNA-lipid-apatite composite layer by controlling the layer molecular composition

“…Note that the present two-parameter approach is based on the molecular composition of the DLp-Ap layer. Combination with different approaches based on the mineral side, i.e., control of ionic composition [18,22,23], micro-morphology [46], and crystal structure [46] of the mineral matrix would be effective in further optimization of the DLp-Ap layer, although this is yet to be clarified.…”

Area-Specific Cell Stimulation via Surface-Mediated Gene Transfer Using Apatite-Based Composite Layers

“…Four kinds of DF-Ap layers were fabricated using the FL and RL reporter genes and the VEGF and BMP-2 differentiation factor genes ( Table 1 ). According to SEM ( Figure 2 ), TF-XRD ( Figure 3 ), ICP ( Figure 4 a), and UV-Vis ( Figure 4 b) results, these layers were similar in their surface morphology, crystalline phase, and the contents of apatite, plasmid DNA, and fibronectin, all of which are controlling factors that affect gene transfer efficiency [ 21 , 25 , 46 ]. The critical difference among these DF-Ap layers lies only on the type of plasmid DNA that was immobilized within the layer.…”