Spontaneous myometrial contractility in cows suffering from endometritis—Influence of localisation, smooth muscle layer and cycle phase. An in vitro study

Hirsbrunner, Gabriela; Kaufmann, Christian A.; Keller, C.; Hüsler, Jürg; Steiner, Adrian

doi:10.1016/j.anireprosci.2009.07.003

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…[3][4][5]. Spontaneous contractility of the cycling non-pregnant uterus has also been studied in the rat [40][41][42][43], guinea pig [44], dog [45], cow [46][47][48][49] and horse [50,51]. We have revealed that despite considerable variability in contractile behaviors, there are certain motility patterns that are more prevalent at each phase of the cycle; changes which likely correspond to the cyclic variation in the levels and receptor expression of ovarian steroid hormones, estrogen and progesterone, which have been shown to modulate spontaneous contractility in vitro [52][53][54][55][56] and in vivo [41][42][43]57].…”

Section: Distinct Patterns Of Uterine Contractility Throughout the Esmentioning

confidence: 99%

Uterine Contractility in the Nonpregnant Mouse: Changes During the Estrous Cycle and Effects of Chloride Channel Blockade1

Dodds

Staikopoulos

Beckett

2015

Biology of Reproduction

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Mechanisms involved in the generation of spontaneous uterine contractions are not fully understood. Kit-expressing interstitial cells of Cajal are pacemakers of contractile rhythm in other visceral organs, and recent studies describe a role for Ca(2+)-activated Cl(-) currents as the initiating conductance in these cells. The existence and role of similar specialized pacemaker cells in the nonpregnant uterus remains undetermined. Spontaneous contractility patterns were characterized throughout the estrous cycle in isolated, nonpregnant mouse uteri using spatiotemporal mapping and tension recordings. During proestrus, estrus, and diestrus, contraction origin predominated in the oviduct end of the uterus, suggesting the existence of a dominant pacemaker site. Propagation speed of contractions during estrus and diestrus were significantly slower than in proestrus and metestrus. Five major patterns of activity were predominantly exhibited in particular stages: quiescent (diestrus), high-frequency phasic (proestrus), low-frequency phasic (estrus), multivariant (metestrus), and complex. Kit-immunopositive cells reminiscent of pacemaking ICCs were not consistently observed within the uterus. Niflumic acid (10 μM), anthracene-9-carboxylic acid (0.1-1 mM), and 5-nitro-2-(3-phenylpropylamino)benzoic acid (10 μM) each reduced the frequency of spontaneous contractions, suggesting involvement of Cl(-) channels in generating spontaneous uterine motor activity. It is unlikely that this conductance is generated by the Ca(2+)-activated Cl(-) channels, anoctamin-1 and CLCA4, as immunohistochemical labeling did not reveal protein expression within muscle or pacemaker cell networks. In summary, these results suggest that spontaneous uterine contractions may be generated by a Kit-negative pacemaker cell type or uterine myocytes, likely involving the activity of a yet-unidentified Cl(-) channel.

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Section: Distinct Patterns Of Uterine Contractility Throughout the Esmentioning

confidence: 99%

Uterine Contractility in the Nonpregnant Mouse: Changes During the Estrous Cycle and Effects of Chloride Channel Blockade1

Dodds

Staikopoulos

Beckett

2015

Biology of Reproduction

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“…Efficient uterine expulsion of fluid and debris involves contractions of the myometrium (Hirsbrunner et al. , ). After bacterial challenge, both susceptible and resistant mares had an increase in myoelectrical activity.…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding‐Induced Endometritis and the Effects of Immunomodulation

Woodward

Christoffersen

Campos

et al. 2012

Reprod Domestic Animals

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The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding-induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post-breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047).

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“…), cow (Ruckebusch & Bayard, ; Hirsbrunner et al . , ; Kaufmann et al . ), horse (Hirsbrunner et al .…”

Section: Discussionmentioning

confidence: 99%

“…In research conducted with the use of telemetry, the surgical technique, the materials used and the design of the electrodes are of vital importance. Many published studies focus on non-pregnant, cycling uterine contractile activity in the rat (Ishikawa & Fuchs, 1978;Downing et al 1981;Crane & Martin, 1991a,b), guinea-pig (Chernaeva & Milenov, 1974), dog (Wheaton et al 1988), cow (Ruckebusch & Bayard, 1975;Hirsbrunner et al 2002Hirsbrunner et al , 2010Kaufmann et al 2008), horse (Hirsbrunner et al 2006) and sow (Mwanza et al 2002;Domino et al 2016Domino et al , 2017aPawlinski et al 2016). The induction and synchronization of oestrus are the most prevalent procedures performed to manage the timing of oestrus to meet the needs of the farm.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method

Pawliński

Domino

Zabielski

et al. 2017

Experimental Physiology

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What is the central question of this study? The aim of present study was to record and analyse the myoelectrical activity in the female pig reproductive tract (uterus and oviduct) during early pregnancy. What is the main finding and its importance? Understanding the contractile activity of the uterus and oviducts is indispensable for understanding the physiological mechanisms as well as all irregularities associated with the period of conception and early pregnancy. The aim of the present study was to record the myoelectrical activity of the reproductive tract in sows during the oestrous phase and early pregnancy via a telemetry recording system. In a total of eight non-pregnant pigs, the bioelectrical activity was recorded through three silicone electrodes sutured on the oviduct (isthmus and ampulla) and the uterine horn. Blood samples were collected to monitor the concentrations of progesterone (P4) and luteinizing hormone (LH). The oestrous cycle was synchronized with equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG), and the animals were subjected to artificial insemination. Analysis of the EMG activity of the oviduct and uterus in the oestrous phase and in early stages of pregnancy suggests explicitly that telemetry could enable in vivo assessment of myoelectrical activity of parts of the reproductive system in sows. Off-line analysis of the duration of EMG activity bursts in the uterus, isthmus and ampulla were significantly higher during early pregnancy (phases II and III) than in the oestrous phase. The EMG signals demonstrated low mean amplitudes of activity in the oviduct and uterus during early pregnancy (phases I-III). Significant differences between the root mean square signals were observed in the isthmus and ampulla both during oestrus and in early pregnancy (phase I; P < 0.01). During the oestrous phase, the P4 concentration was estimated at <1 ng ml , whereas the LH concentration was >4 ng ml . In contrast, during early pregnancy, the P4 and LH concentrations were estimated at >4 and <1 ng ml , respectively.

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Spontaneous myometrial contractility in cows suffering from endometritis—Influence of localisation, smooth muscle layer and cycle phase. An in vitro study

Cited by 6 publications

References 35 publications

Uterine Contractility in the Nonpregnant Mouse: Changes During the Estrous Cycle and Effects of Chloride Channel Blockade1

Uterine Contractility in the Nonpregnant Mouse: Changes During the Estrous Cycle and Effects of Chloride Channel Blockade1

An Investigation of Uterine Nitric Oxide Production in Mares Susceptible and Resistant to Persistent Breeding‐Induced Endometritis and the Effects of Immunomodulation

Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method

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