Spontaneous Tumors and Pathologic Lesions in SWR/J Mice23

Rabstein, Louise S.; Peters, Robert L.; Spahn, Gerard J.

doi:10.1093/jnci/50.3.751

Cited by 37 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11,[22][23][24][25][26] Hyalinosis in gall bladders and in bile and pancreatic ducts has also been reported previously in these same and several other mouse strains or stocks. 11,13,14,[27][28][29] The characteristic pulmonary lesions containing macrophages with eosinophilic crystal formation seen in our 129S4/SvJae population, furthermore, resemble those reported in the motheaten mouse 16,22,30,31 and are sometimes associated with lung tumors 32,33 as well as with macrophage pneumonias in a number of strains. 22,27 In motheaten mice, the pulmonary macrophage lesion is associated with a deficiency of SHP-1, a protein tyrosine phosphatase.…”

Section: Discussionsupporting

confidence: 53%

Hyalinosis and Ym1/Ym2 Gene Expression in the Stomach and Respiratory Tract of 129S4/SvJae and Wild-Type and CYP1A2-Null B6,129 Mice

Ward

Yoon

Anver

et al. 2001

The American Journal of Pathology

127

129

View full text Add to dashboard Cite

The C57BL/6, 129, and B6,129 mouse strains or stocks have been commonly used to generate targeted mutant mice. The pathology of these mice is not well characterized. In studies of these aging mice, we found high incidences of hyalinosis (eosinophilic cytoplasmic change) in the glandular stomach, respiratory tract, bile duct, and gall bladder of B6,129 CYP1A2-null and wild-type mice as well as in both sexes of the background 129S4/SvJae strain. The gastric lesions of the glandular stomach were found in 95.7% of female CYP1A2-null mice as well as in 45.7% of female 129S4/SvJae animals. The eosinophilic protein isolated from characteristic hyaline gastric lesions was identified as Ym2, a member of the chitinase family. Immunohistochemistry, using rabbit polyclonal antibodies to oligopeptides derived from the Ym1 sequence, detected focal to diffuse reactivity within both normal and abnormal nasal olfactory and respiratory epithelium, pulmonary alveolar macrophages, bone marrow myeloid cells, and the squamous epithelium of the forestomach and epithelium of the glandular stomach. Alveolar macrophages in acidophilic pneumonia, a major cause of death of aging 129 mice, and in mice with the me mutation also were highly immunoreactive. CYP1A2 carries out oxidation and N-hydroxylation of arylamine carcinogens and heterocyclic amine food mutagens which, when followed by O-esterification by acetate or sulfate transferases, results in unstable electrophilic derivatives that can cause cell toxicity, cell death, or cell transformation. 1-3 CYP1A2 catalyzes caffeine 3-demethylation and metabolically activates aflatoxin B 1 to its ultimate carcinogenic intermediate 4 and paradoxically catalyzing the hydroxylation of aflatoxin B 1 to aflatoxin M 1 in a detoxification pathway. 5 In addition, CYP1A2 was found to be involved in both hamster and human catechol estrogen metabolism. 6 Although CYP1A2 is constitutively expressed in the liver of mice, rats, and humans and is inducible by ligands of the aryl hydrocarbon receptor (Ahr) in all mammalian species analyzed, no expression has been demonstrated in the stomach, even after treating animals with known inducers of this enzyme. 7 However, the mechanisms involved in CYP1A2 gene regulation are less well established than CYP1A1 because it is difficult to induce expression in vitro and the sensitivities of current methodologies may simply be too low under normal in vivo conditions. The mammalian conservation of the CYP1A subfamily argues for an essential function in metabolism not limited to activation and detoxification of exogenous chemicals and toxins. As with most of the cytochromes P-450, determination of function has primarily been a consequence of exposures to man-made compounds, but it must be remembered that their evolution predates industrialization. The genes of the two recognized members of the CYP1A subfamily,

show abstract

Section: Discussionsupporting

confidence: 53%

Hyalinosis and Ym1/Ym2 Gene Expression in the Stomach and Respiratory Tract of 129S4/SvJae and Wild-Type and CYP1A2-Null B6,129 Mice

Ward

Yoon

Anver

et al. 2001

The American Journal of Pathology

127

129

View full text Add to dashboard Cite

show abstract

“…Prostatic neoplasms were not detected in our study, in contrast to that of F-344 rats in which spontaneous neoplasms were observed in 0.9% of ventral lobes examined (20); however, adenomas and adenocarcinomas were present in the seminal vesicles of B6C3F 1 mice but absent in the F-344 rat (20). Spontaneous proliferative lesions including hyperplasia and adenoma have not been reported in the ampullary gland in mice (2,5,7,(11)(12)(13)(14)(21)(22)(23), nor were they observed in this evaluation. Spontaneous granular cell tumors in the seminal vesicles have been previously reported only in NMRI and B6C3F 1 mice (9,15).…”

Section: Introductioncontrasting

confidence: 60%

Spontaneous Lesions in Control B6C3F1 Mice and Recommended Sectioning of Male Accessory Sex Organs

Suwa¹,

Nyska²,

Haseman

et al. 2002

Toxicol Pathol

View full text Add to dashboard Cite

Because sampling of the paired lobes (ventral, dorsal, lateral, and anterior) of the mouse prostate has often been inconsistent , comparisons among different investigations have lacked validity. The absence of site identi cation for prostatic lesions has made reported incidences relatively nonspeci c. We present here the lobe-speci c incidences and degree of severity of spontaneou s lesions in prostate, coagulating gland (anterior prostatic lobe), seminal vesicles, and ampullary glands in 612 control B6C3F 1 mice from 12 recent National Toxicology Program 2-year carcinogenicity and toxicity studies conducted in 1 of 4 different laboratories. Lymphocyti c in ltration, in ammation, epithelial hyperplasia, mucinous cyst, and mucinous metaplasia were observed in the dorsolateral lobes. Lymphocytic in ltration, in ammation, epithelial hyperplasia, and edema were present in the ventral lobes. Lymphocytic in ltration, acinar dilatation, in ammation, epithelial hyperplasia, and atrophy occurred in the coagulating glands. No neoplastic lesions were observed in the prostate or coagulating gland. Lymphocytic in ltration, acinar dilatation, in ammation, atrophy, adenoma, adenocarcinoma , and a granular cell tumor were observed in the seminal vesicles. Lymphocytic in ltration was also present in the ampullary glands. The results of our survey indicate that the amounts of glandular tissues were not present consistently in slides from the different laboratories. Landmarks for uniform tissue trimming are needed. We therefore suggest an optimal trimming and embedding method for mouse prostate and seminal vesicles to ensure adequate, consistent sampling.

show abstract

“…For example, Rabstein et al reported a similar acidophilic substance in the biliary epithelial cells of SWR/J mice, which were described as showing nonmalignant adenomatous changes. 17 Enomoto et al reported the deposition of similar acidophilic substances and crystals in the epithelium of the gallbladder and extrahepatic bile duct of nontreated DDD mice. 18 Nakao et al reported similar changes in the biliary and pancreatic duct epithelial cells and bronchial epithelial cells of CD-1 mice.…”

Section: Discussionmentioning

confidence: 99%

Aly/Aly mice: A unique model of biliary disease

et al. 1998

View full text Add to dashboard Cite

An autosomal recessive murine mutation, coined ''aly/ aly'' or ''alymphoplasia,'' was recently reported. Homozygotes for aly are defective in both humoral and cellmediated immune function and have diffuse lymphoid cell infiltration of various tissues, particularly around the conduit ducts of the pancreas and salivary glands. In pilot studies in our laboratories, aly/aly mice were found to have peculiar biliary tract lesions, which were analyzed histologically and immunohistochemically in the present study. The livers of aly/aly mice older than 8 weeks consistently showed a variable lymphoid cell infiltration with lymph follicle formation in portal tracts; intrahepatic biliary epithelial cells showed various types of damage including pseudopyloric gland metaplasia and proliferative changes. In addition, the extrahepatic bile duct and intrahepatic large bile duct were found to contain an acidophilic substance in their epithelial cytoplasm. In the lumen and occasionally in the cytoplasm of these bile ducts, acidophilic crystals were also seen. Ultrastructurally, the intracytoplasmic acidophilic substances consisted of membrane-bound intracytoplasmic inclusions with homogeneous electron density, likely derived from rough endoplasmic reticulum (ER). Immunohistochemically, the cytoplasmic acidophilic substances were simultaneously positive for cystatin C, gastrin, serotonin, and somatostatin. In contrast, the acidophilic crystals did not react with any of these antibodies. These findings suggest that the intracytoplasmic acidophilic substances may contain a precursor of the peptide hormones, possibly because of defective secretion or intracellular transport. We believe that the aly/aly mouse is a useful model for the analysis of biliary metabolic events, and for studies of the interaction of the immune system and biliary destruction. (HEPATOLOGY 1998;27:1499-1507.)

show abstract

Spontaneous Tumors and Pathologic Lesions in SWR/J Mice23

Cited by 37 publications

References 0 publications

Hyalinosis and Ym1/Ym2 Gene Expression in the Stomach and Respiratory Tract of 129S4/SvJae and Wild-Type and CYP1A2-Null B6,129 Mice

Hyalinosis and Ym1/Ym2 Gene Expression in the Stomach and Respiratory Tract of 129S4/SvJae and Wild-Type and CYP1A2-Null B6,129 Mice

Spontaneous Lesions in Control B6C3F1 Mice and Recommended Sectioning of Male Accessory Sex Organs

Aly/Aly mice: A unique model of biliary disease

Contact Info

Product

Resources

About