Spontaneous VEGF Production by Cultured Peritoneal Mesothelial Cells from Patients on Peritoneal Dialysis

Abstract

Episodes of peritonitis 1.4±2.6 0.8±1.3 0.6 Accumulated glucose load (kg) 156.1±287 84.3±66 0.6 Creatinine MTC (mL/min) 11.7±9 10.1±3 0.7 Urea MTC (mL/min) 22.1±13 24.4±7.1 0.7 Ultrafiltration (mL/4 hr, 2.27% glucose) 650±339 540±124 0.5 VEGR = vascular endothelial growth factor; PD = peritoneal dialysis; MTC = mass transfer coefficient.

Epithelial-to-mesenchymal transition of mesothelial cells is an early event during peritoneal dialysis and is associated with high peritoneal transport

“…Recently, important contributions from cell biology and histopathology studies have helped us to understand the pathophysiology of the peritoneal membrane response. [12][13][14][15][16][17][18] Epithelial-to-mesenchymal transition (EMT) of MC has been identified as a key process leading to peritoneal fibrosis with functional deterioration. 19,20 Concerning human histopathology studies, it must be remarked that most peritoneal biopsy studies have been based on PD patients with long-term treatment and peritoneal functional problems, specifically UF failure.…”

Peritoneal function in clinical practice: the importance of follow-up and its measurement in patients. Recommendations for patient information and measurement of peritoneal function

“…Cultured mesothelial cells synthesize various chemokines, prostaglandins and growth factors [ 29 ]. Some are produced constitutively, like vascular endothelial growth factor (VEGF) [ 30 ] and cancer antigen 125 (CA 125) [ 31 ]. Others, like interleukin-6 (IL-6) and IL-8, are produced after stimulation with chemokines [ 32 , 34 ].…”

Opinion: What Are the Problems with Using the Peritoneal Membrane for Long‐Term Dialysis?

“…In vitro studies have shown that cultured mesothelial cells are able to produce various cytokines, chemokines, and prostaglandins (15). Most inflammatory mediators, like interleukin (IL)‐6 and IL‐8 are produced after stimulation with tumor necrosis factor‐α (TNF‐α) or IL‐1; others like vascular endothelial growth factor (VEGF) and cancer antigen 125 (CA125) are released constitutively (16,17). All these substances, some of which are vasoactive, can be detected in peritoneal effluent of PD patients (18).…”