Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia

Kumsa, Bersissa; Socolovschi, Cristina; Raoult, Didier; Parola, Philippe

doi:10.1016/j.ttbdis.2014.08.001

Cited by 31 publications

(36 citation statements)

References 33 publications

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“…Ixodid ticks were collected from cattle, sheep, dogs, and cats in the Arsi, Wolmara, Kimbibit Ada'a, Abdela, Gachi, Arero, Moyale, and Yabelo districts in Oromia as described in a previous publication. 5 These districts are located in six zones of the central, southwestern, and southeast parts of Ethiopia with various climates and agro-ecological and animal management systems (Figure 1). Livestock comprising cattle, goats, sheep, and camels in Arero, Moyale, and Yabelo districts (Borana zone) are kept under a pastoral type of animal management.…”

Section: Methodsmentioning

confidence: 99%

“…All the body surfaces of each study animal were thoroughly examined visually to establish the presence or absence of ticks. 5 Ticks attached to the skin of each animal were carefully removed manually using forceps or by hand to avoid any damage to the body and placed into separate, prelabeled, small, plastic tubes containing 70% ethanol for subsequent identification as previously Figure 1. Map of the Oromia State showing study districts in Ethiopia with the overall prevalence of C. burnetii, positive tick spp., and multispacer sequence typing (MST) recorded in each district.…”

Section: Methodsmentioning

confidence: 99%

“…described. 5 Ticks from the same animal were put in the same vial and transported to the laboratory (URMITE, Marseille, France). Ticks were morphologically identified under a microscope using identification keys described previously.…”

Section: Methodsmentioning

confidence: 99%

“…Before DNA extraction, each tick specimen was rinsed twice in sterile water for 15 minutes and then dried on sterile filter paper. 5 Each specimen was longitudinally cut into two equal halves. 32,33 One half of each specimen was kept as reserve sample to avoid the risk of losing samples for any reason during or after DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

“…4 Most previously published data on ticks in Ethiopia focus on species distribution, and thus only a very few molecular studies have been conducted on the occurrence of pathogens in ticks in the country. 5 Coxiella burnetii, the causative agent of Q fever, is a small (3-5 μm) polymorphic obligate intracellular gram-negative bacteria belonging to the genus Coxiella, family Coxiellaceae, order Legionellales, class Gammaproteobacteria, and phylum Proteobacteria.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and Genotyping of Coxiella burnetii in Ixodid Ticks in Oromia, Ethiopia

Kumsa¹,

Socolovschi²,

Almeras³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. This study was conducted from September 2011 to March 2014 to address the occurrence and genotypes of Coxiella burnetii using molecular methods in ticks collected from domestic animals in Ethiopia. Ticks were tested for C. burnetii by quantitative real-time polymerase chain reaction (qPCR) targeting two different genes followed by multispacer sequence typing (MST). An overall prevalence of 6.4% (54/842) of C. burnetii was recorded. C. burnetii was detected in 28.6% (14/49) of Amblyomma gemma, 25% (31/124) of Rhipicephalus pulchellus, 7.1% (1/14) of Hyalomma marginatum rufipes, 3.2% (2/62) of Am. variegatum, 3.1% (4/128) of Am. cohaerens, 1.6% (1/63) of Rh. praetextatus, and 0.6% (1/153) of Rhipicephalus (Boophilus) decoloratus. Significantly higher overall frequencies of C. burnetii DNA were observed in Am. gemma and Rh. pulchellus than in other tick species (Mantel-Haenszel [MH], P 0.0001). The overall frequency of C. burnetii was significantly higher (MH, P 0.0001) in ticks from southeastern districts (Arero, Moyale, and Yabelo) than that from other districts. This study demonstrated the presence of C. burnetii genotype MST 18 in ticks in southeastern districts and genotype MST 20 in ticks in central districts. This study highlights the importance of ticks in the epidemiology of C. burnetii in Ethiopia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and Genotyping of Coxiella burnetii in Ixodid Ticks in Oromia, Ethiopia

Kumsa¹,

Socolovschi²,

Almeras³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Multi‐country investigation of the diversity and associated microorganisms isolated from tick species from domestic animals, wildlife and vegetation in selected african countries

et al. 2021

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In many areas of Africa, recent studies highlighted the great impact of ticks on animal and human health throughout the continent. On the other hand, very limited information on the bacterial endosymbionts of the African ticks and their pattern of co-infections with other bacteria are found in literature, notwithstanding their pivotal role in tick survival and vector efficiency. Thus, we investigated the distribution of selected pathogenic and symbiotic bacteria in hard ticks collected from wild, domestic animals and from vegetation in various ecological zones in Africa and their co-occurrence in the same tick host. Overall, 339 hard ticks were morphologically identified as belonging to the genera Amblyomma, Dermacentor, Hyalomma, Haemaphysalis, Ixodes and Rhipicephalus. Molecular screening provided information on pathogens circulation in Africa, detecting spotted fever group rickettsiae, Anaplasma spp., Ehrlichia ruminantium, Borrelia garinii, Babesia spp., Theileria spp. and Coxiella burnetii. Furthermore, our work provides insights on the African scenario of tick-symbiont associations, revealing the presence of Coxiella, Francisella and Midichloria across multiple tick populations. Coxiella endosymbionts were the most prevalent microorganisms, and that with the broadest spectrum of hosts, being detected in 16 tick species. Francisella was highly prevalent among the Hyalomma species tested and correlated negatively with the presence of Coxiella, showing a potential competitive interaction. Interestingly, we detected a positive association of Francisella with Rickettsia in specimens of Hy. rufipes, suggesting a synergistic interaction between them. Finally, Midichloria was the most prevalent symbiont in Rhipicephalus sanguineus sensu lato from Egypt.

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Tick species from cattle in the Adama Region of Ethiopia and pathogens detected

et al. 2021

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Ticks will diminish productivity among farm animals and transmit zoonotic diseases. We conducted a study to identify tick species infesting slaughter bulls from Adama City and to screen them for tick-borne pathogens. In 2016, 291 ticks were collected from 37 bulls in Adama, which were ready for slaughter. Ticks were identified morphologically. Total genomic DNA was extracted from ticks and used to test for Rickettsia spp. with real-time PCR. Species identification was done by phylogenetic analysis using sequencing that targeted the 23S-5S intergenic spacer region and ompA genes. Four tick species from two genera, Amblyomma and Rhipicephalus, were identified. Amblyomma cohaerens was the dominant species (n = 241, 82.8%), followed by Amblyomma variegatum (n = 22, 7.5%), Rhipicephalus pulchellus (n = 19, 6.5%), and Rhipicephalus decoloratus (n = 9, 3.0%). Among all ticks, 32 (11%) were positive for Rickettsia spp. and 15 (5.2%) of these were identified as R. africae comprising at least two genetic clades, occurring in A. variegatum (n = 10) and A. cohaerens (n = 5). The remainder of Rickettsia-positive samples could not be amplified due to low DNA yield. Furthermore, another 15 (5.2%) samples carried other pathogenic bacteria: Ehrlichia ruminantium (n = 9; 3.1%) in A. cohaerens, Ehrlichia sp. (n = 3; 1%) in Rh. pulchellus and A. cohaerens, Anaplasma sp. (n = 1; 0.5%) in A. cohaerens, and Neoehrlichia mikurensis (n = 2; 0.7%) in A. cohaerens. All ticks were negative for Bartonella spp., Babesia spp., Theileria spp., and Hepatozoon spp. We reported for the first time E. ruminatium, N. mikurensis, Ehrlichia sp., and Anaplasma sp. in A. cohaerens. Medically and veterinarily important pathogens were mostly detected from A. variegatum and A. cohaerens. These data are relevant for a One-health approach for monitoring and prevention of tick-borne disease transmission.

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Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia

Cited by 31 publications

References 33 publications

Occurrence and Genotyping of Coxiella burnetii in Ixodid Ticks in Oromia, Ethiopia

Occurrence and Genotyping of Coxiella burnetii in Ixodid Ticks in Oromia, Ethiopia

Multi‐country investigation of the diversity and associated microorganisms isolated from tick species from domestic animals, wildlife and vegetation in selected african countries

Tick species from cattle in the Adama Region of Ethiopia and pathogens detected

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