SPR Sensor Instrumentation

Piliarik, Marek; Homola, Jiřı́

doi:10.1007/5346_016

Cited by 25 publications

(18 citation statements)

References 72 publications

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“…The smallest change in mass surface density that can be detected is estimated to be 300 pg cm 22 considering the lowest detectable Dk SPR is three times the standard deviation of the baseline noise. This value is consistent with other systems which were capable of detecting biomolecules (Piliarik and Homola. 2006).…”

Section: Underwater Characterization Of the Transducersupporting

confidence: 92%

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A surface plasmon resonance system for the underwater detection of domoic acid

Colas

Crassous

Laurent

et al. 2016

Limnology & Ocean Methods

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Over the past decade Surface Plasmon Resonance (SPR) techniques have been applied to the measurement of numerous analytes. In this article, an SPR biosensor system deployed from an oceanographic vessel was used to measure dissolved domoic acid (DA), a common and harmful phycotoxin produced by certain microalgae species belonging to the genus Pseudo-nitzschia. During the biosensor deployment, concentrations of Pseudo-nitzschia cells were very low over the study area and measured DA concentrations were below detection. However, the in situ operational detection limit of the system was established using calibrated seawater solutions spiked with DA. The system could detect the toxin at concentrations as low as 0.1 ng mL 21 and presented a linear dynamic range from 0.1 ng mL 21 to 2.0 ng mL 21 . This sensor showed promise for in situ detection of DA.

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Section: Underwater Characterization Of the Transducersupporting

confidence: 92%

“…This value is consistent with other systems which were capable of detecting biomolecules (Piliarik and Homola. ).…”

Section: Assessmentmentioning

confidence: 97%

A surface plasmon resonance system for the underwater detection of domoic acid

Colas

Crassous

Laurent

et al. 2016

Limnology & Ocean Methods

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“…Commercial SPR sensors can incorporate 400 unique receptors on a single chip. 8 In academic studies, SPR arrays are being developed to analyze RNA, 9 viruses, 10 proteins, 11 carbohydrates, 12 and small molecules. 13 …”

Section: Introductionmentioning

confidence: 99%

A Localized Surface Plasmon Resonance Imaging Instrument for Multiplexed Biosensing

et al. 2013

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Localized surface plasmon resonance (LSPR) spectroscopy has been widely used for label-free, highly-sensitive measurements of interactions at a surface. LSPR imaging (LSPRi) has the full advantages of LSPR, but enables high-throughput, multiplexed measurements by simultaneously probing multiple individually addressable sensors on a single sample surface. Each spatially distinct sensor can be tailored to provide data regarding different surface functionalities or reaction environments. Previously, LSPRi has focused on single particle sensing where the size scale is very small. Here, we create defined macroscale arrays of nanoparticles that are compatible with common patterning methods such as dip-pen nanolithography and multi-channel microfluidic delivery devices. With this new LSPR sensing format, we report the first demonstration of multiplexed LSPR imaging and show that the increased throughput of our instrument enables the collection of a complete Langmuir binding curve on a single sensor surface. In addition, the multiplexed LSPR sensor is highly selective, as demonstrated by the hybridization of single-stranded DNA to complementary sequences immobilized on the sensor surface. The LSPR arrays described in this work exhibit uniform sensitivity and tailorable optical properties, making them an ideal platform for high-throughput, label-free analysis of a variety of molecular binding interactions.

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“…Binding events that change the refractive index at the gold-solution interface are measured either as a change in the angle or wavelength of light which is maximally coupled into the metal film. SPR has been actively investigated as a biosensing technique over the past several decades with a number of reported sensor geometries and modes of operation, several of which are available commercially (Homola 2008; Piliarik and Homola 2006; Scarano et al 2010). For SPR sensors, the evanescent field penetration depth, which is a function of wavelength and dielectric environment, is on the order of hundreds of nanometers (Homola 2006) with bulk refractive index sensitivities usually varying between 10 -5 and 10 -7 RIU (Fu et al 2008; Homola 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform

Luchansky

Washburn

Martin

et al. 2010

Biosensors and Bioelectronics

104

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Silicon photonic microring resonators have emerged as a sensitive and highly multiplexed platform for real-time biomolecule detection. Herein, we profile the evanescent decay of device sensitivity towards molecular binding as a function of distance from the microring surface. By growing multilayers of electrostatically bound polymers extending from the sensor surface, we are able to empirically determine that the evanescent field intensity is characterized by a 1/e response decay distance of 63 nm. We then applied this knowledge to study the growth of biomolecular assemblies consisting of alternating layers of biotinylated antibody and streptavidin, which follow a more complex growth pattern. Additionally, by monitoring the shift in microring resonance wavelength upon the deposition of a radioactively labeled protein, the mass sensitivity of the ring resonator platform was determined to be 14.7 ± 6.7 [pg/mm 2 ]/Δpm. By extrapolating to the instrument noise baseline, the absolute mass limit of detection is found to be 1.5 ± 0.7 pg/mm 2 . Taking the small surface area of the microring sensor into consideration, this value corresponds to an absolute mass detection limit of 97 ag (i.e. 0.6 zmol of IgG), demonstrating the remarkable sensitivity of this promising label-free biomolecular sensing platform.

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SPR Sensor Instrumentation

Cited by 25 publications

References 72 publications

A surface plasmon resonance system for the underwater detection of domoic acid

A surface plasmon resonance system for the underwater detection of domoic acid

A Localized Surface Plasmon Resonance Imaging Instrument for Multiplexed Biosensing

Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform

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