Spreeta-Based Biosensor Immunoassays to Detect Fraudulent Adulteration in Milk and Milk Powder

Haasnoot, W.; Marchesini, Gerardo; Koopal, Kees

doi:10.1093/jaoac/89.3.849

Cited by 33 publications

(16 citation statements)

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“…The technology's breadth of applications is further illustrated by the range of articles investigating oligonucleotides , small molecules , carbohydrates , and extracellular matrix components . In addition, the number of articles describing biosensor work in clinical support [1008][1009][1010][1011][1012][1013][1014][1015][1016][1017][1018][1019][1020][1021][1022][1023][1024], food/agricultural/veterinary/environmental sciences [1025][1026][1027][1028][1029][1030][1031][1032][1033][1034][1035][1036][1037][1038], and membrane/virus/cell characterization [1039][1040][1041][1042][1043][1044][1045][1046][1047][1048][1049][1050] demonstrate Biacore's emerging contributions in these fields. Biacore's more unusual applications, including the characterization of fibrils and the analysis of crude analytes are summarized in References 1051-1066.…”

Section: Methodsmentioning

confidence: 99%

Survey of the year 2006 commercial optical biosensor literature

Rich

Myszka

2007

J of Molecular Recognition

110

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We identified 1219 articles published in 2006 that described work performed using commercial optical biosensor platforms. It is interesting to witness how the biosensor market is maturing with an increased number of instrument manufacturers offering a wider variety of platforms. However, it is clear from a review of the results presented that the advances in technology are outpacing the skill level of the average biosensor user. While we can track a gradual improvement in the quality of the published work, we clearly have a long way to go before we capitalize on the full potential of biosensor technology. To illustrate what is right with the biosensor literature, we highlight the work of 10 groups who have their eye on the ball. To help out the rest of us who have the lights on but nobody home, we use the literature to address common myths about biosensor technology.

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Section: Methodsmentioning

confidence: 99%

Survey of the year 2006 commercial optical biosensor literature

Rich

Myszka

2007

J of Molecular Recognition

110

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“…Sometimes milk fat is replaced by fat from other sources which may also pose a risk to human health. [9] Low-Valued Milk-Milk is adulterated by mixing lower-valued milk with higher valued milk. For example, often goat milk is adulterated with cow milk for greater profit.…”

Section: Purpose Of the Projectmentioning

confidence: 99%

Smart Milk Adulteration Detection

Chandnani*¹,

Belani²,

Bansal³

et al. 2019

IJRTE

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Adulteration is extremely basic today and the most normally adulterated nourishment item is milk. The most widely recognized explanation behind adulteration is the utilization by producers of undeclared materials that are less expensive than the right and pronounced ones. Adulteration brings down the nature of nourishment and now and then, harmful synthetic substances are likewise added which can be dangerous to health. Adulteration of products either in liquid or solid form can harm or pose serious health risk issues to the consumer. The present investigation was arranged with the primary goal of recognizing purchasing practices of homemakers and their degree of awareness identified with chosen food items. Being considerate of the health & betterment of the society we have planned a project which will allow the customer to know about the level of purity of product which they are buying and being aware of it in future.

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“…However, the applied optical biosensor (Biacore 3000) is very expensive and not suitable for routine control application. An alternative and cheaper sensor (Spreeta) has also been applied to this purpose [13] but is not commercially available. The Mabs were previously applied in an indirect ELISA format for the detection of bovine rennet whey powder in milk powder and buttermilk powder [14] using a ĸ-casein-coated plate.…”

Section: Introductionmentioning

confidence: 99%

ELISA for Raw and Heat-Treated Cows and Buffalos Milk in the Milk of Other Species and Sources

Sajic¹,

Essers²

2015

J Adv Dairy Res

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Due to the lower prices of cow's and buffalo's milk, fraudulent mixing with higher priced milk of other species and sources is economically attractive but illegal and dangerous for allergic consumers. For the rapid detection of cow's milk, several immunoassays are available but most of them lack the possibility to detect heat-treated cow's milk due to denaturation of the target protein(s). In the present indirect enzyme-linked immunosorbent assay (ELISA), a mouse monoclonal antibody (Mab) raised against the bovine milk protein ĸ-casein is labelled with the enzyme horseradish peroxidase and the binding to the bovine ĸ-casein-coated wells of a 96-wells microtiter plate is inhibited by free bovine ĸ-casein in the sample or standard. The Mab recognises a 5 amino acids-containing epitope on the glycomacropeptide (GMP) part of bovine ĸ-casein which is absent in ĸ-casein of goat, sheep, horse, donkey, camel, etc, but is present in ĸ-casein of buffalo. Due to the indirect assay format, the cow's and buffalo's milk ELISA also recognises heat-treated milk containing denatured ĸ-casein. The measurement range of the bovine ĸ-casein calibration standards is from 0.1 to 2.5 µg/ml, the limit of detection is 0.05 µg/ml and the only milk sample preparation needed is dilution (100 or 1000 times). This new fast and easy-to-apply ELISA is well suited for the detection of the lower priced raw and heated milk of cows and buffalos in the higher priced milk of other species and sources within the range of 0.25 till 50%.

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Spreeta-Based Biosensor Immunoassays to Detect Fraudulent Adulteration in Milk and Milk Powder

Cited by 33 publications

References 0 publications

Survey of the year 2006 commercial optical biosensor literature

Survey of the year 2006 commercial optical biosensor literature

Smart Milk Adulteration Detection

ELISA for Raw and Heat-Treated Cows and Buffalos Milk in the Milk of Other Species and Sources

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