Squamous cell carcinoma antigen is a new member of the serine protease inhibitors

Suminami, Yoshinori; Kishi, Fumio; Sekiguchi, Kazuma; Katô, Hiroshi

doi:10.1016/s0006-291x(05)81380-4

Cited by 194 publications

(179 citation statements)

References 25 publications

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“…RT-PCR was carried out using the specific primer for leupin as described above or with a specific primer corresponding to the unique SCCA reactive site loop region sequence [8], primer E: 5" CCCCCGGGTA-CCTACGGGGATGAATCTG 3' (antisense to nucleotides 1054-1074 in SCCA). In each case, amplification with the sense primer D to the 5' open reading frame sequence was performed to yield a 1094 bp product.…”

Section: Expression Of Scca and Leupinmentioning

confidence: 99%

“…A sub-family of serpins related to ovalbumin, the ov-serpins, has been identified based on higher sequence identity (45 55%), gene organisation, and lack of an identifiable N-terminal signal sequence [4]. Human proteins belonging to this ov-serpin family include plasminogen activitor inhibitor-2 (PAI-2) [5], leucocyte elastase inhibitor (LEI) [6], placental thrombin inhibitor (PTI) [7], and squamous cell carcinoma antigen (SCCA) [8].…”

Section: Introductionmentioning

confidence: 99%

“…SCCA isolated from carcinoma of the cervix demonstrates heterogeneity on isoelectric focussing, with normal squamous epithelia contairfing a predominantely neutral form and carcinoma tissue containing both neutral and acidic forms [13]. The gene encoding for SCCA has been cloned and sequenced from SKGIIIa cells, a uterine cervical carcinoma cell line [8]. The cleavage site of SCCA is Se~Ser which is unique to this serpin, and the protein has recently been shown to have inhibitory activity against cathepsin L and papain which are cysteine rather than serine proteases [14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of a novel human serpin gene; cloning sequencing and expression of leupin

Barnes

Worrall

1995

FEBS Letters

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Section: Expression Of Scca and Leupinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of a novel human serpin gene; cloning sequencing and expression of leupin

Barnes

Worrall

1995

FEBS Letters

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“…9 SCCA is a component of the high molecular weight serine protease inhibitors named serpins. 10 A different expression of SCCA in HCC and peritumoral tissue has recently been documented, together with a higher concentration of this antigen in the serum of HCC patients than cirrhotic patients. 11 SCCA is physiologically expressed in the squamous epithelia, and increased levels have been detected in several epithelial cancers such as those of the head, neck, cervix and lung.…”

mentioning

confidence: 99%

SCCA antigen combined with alpha‐fetoprotein as serologic markers of HCC

Giannelli

Marinosci

Trerotoli

et al. 2005

Intl Journal of Cancer

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Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Because of its increased incidence in the last decade and the estimated further increase in the next 2 decades, HCC is arousing great interest. In Europe and North America, it commonly develops on cirrhotic livers, and surveillance programs have therefore been suggested to identify early HCC, at a stage when it remains suitable for surgical therapy and has a better clinical outcome. The only serologic marker used in clinical practice is a-fetoprotein (a-FP), but its sensitivity is poor. In our study, 120 patients with HCC and 90 patients with liver cirrhosis were investigated. We report for the first time to our knowledge that as a marker of HCC, the squamous cell carcinoma (SCCA) antigen has high sensitivity (84.2%) but low specificity (48.9%). However, the combination of a-FP and SCCA yielded a correct serologic diagnosis in 90.83% of the HCC patients. A small percentage of patients remain undetected, likely because of the low specificity of SCCA. In conclusion, the combined use of a-FP and SCCA antigen represents a more powerful tool for the serologic detection of HCC. ' 2005 Wiley-Liss, Inc.Key words: SCCA; HCC; a-fetoprotein; diagnosis; cirrhosis Hepatocellular carcinoma (HCC) is becoming a major health problem worldwide as it represents the fifth most common cancer in the world and the third most common cause of cancer-related death. In Europe and the USA, the incidence rates of HCC have strongly increased in the last decade and will likely increase further in the upcoming 2 decades due to hepatitis C virus infection, 1,2 although there is some controversy as to whether it is directly responsible for liver cancer development. [3][4][5] In Western and North American countries, HCC commonly develops in cirrhotic livers whatever the etiology, so that liver cirrhosis by itself represents the strongest risk factor. 3,5,6 The clinical outcome and the prognosis of HCC are unsatisfactory, even if in developed countries a major improvement of treatment and survival has been achieved in patients with HCC at the initial stage. 7 Therefore, surveillance programs aimed at detecting early stage HCC have been recommended by the European Association for the Study of the Liver (EASL) as well as the Italian Association for the Study of the Liver (AISF). These programs are based on the use of ultrasound tomography and a-fetoprotein (a-FP). The reliability of imaging techniques has greatly improved in the last years but such diagnostic procedures are expensive and subject to interpretation. On the other hand, as the only diagnostic serologic test currently available in clinical practice, a-FP has too low a sensitivity and specificity; based on receiving operating characteristic (ROC) curve analysis, its sensitivity reaches only 60%. 8 It has been reported that the squamous cell carcinoma antigen (SCCA) is overexpressed in HCC tissues. 9 SCCA is a component of the high molecular weight serine protease inhibitors named serpins. 10 A different expressi...

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“…There are evidences which proposes HBV-BP to be SCCA1 as HBV-BP has been found to inhibit cysteine proteinase such as cathepsin L and papain like SCCA1 [11]. It modulates human immune responses towards parasites derived proteases and tumors [15,65].…”

Section: Hepatitis B Virus Binding Protein (Hbv-bp)mentioning

confidence: 99%

Deciphering the mystery of hepatitis B virus receptors: A historical perspective

2015

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Hepatitis B virus is one of the major reasons of viral hepatitis with an estimated 350 million infected patients worldwide. Although, the virus was discovered and cloned more than three decades ago, its entry mechanism has still been in investigation. Numerous potential candidates have been proposed and investigated rigorously to reveal HBV entry mechanism and to unveil the first door of viral entry to hepatocytes. This review provides a short account of role of receptors for entry of HBV into hepatocytes. The viral preS1 region of large surface protein is involved in the attachment of HBV to hepatocytes. The putative attachment site of HBV is located at amino acids 21-47 of preS1. So far, several proteins have been proposed to interact with these different regions of the preS1 domain which includes human immunoglobulin A receptor, glyceraldehyde-3-phosphate dehydrogenase, interleukin-6, a 31-kDa protein, HBV binding factor, asialoglycoprotein receptor, nascent polypeptide-associated complex a polypeptide, lipoprotein lipase, hepatocyteassociated heparan sulfate proteoglycans, glucose-regulated protein 75. However, none of them have appeared to be generally accepted as a true receptor for the virus until recently when sodium taurocholate cotransporting polypeptide identified as HBV entry receptor. Current review provides scientific historical perspective of various candidates known to be interacting with preS1 of HBV for their possible role in viral entry.

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Squamous cell carcinoma antigen is a new member of the serine protease inhibitors

Cited by 194 publications

References 25 publications

Identification of a novel human serpin gene; cloning sequencing and expression of leupin

Identification of a novel human serpin gene; cloning sequencing and expression of leupin

SCCA antigen combined with alpha‐fetoprotein as serologic markers of HCC

Deciphering the mystery of hepatitis B virus receptors: A historical perspective

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