Squash Leaf Curl, a New Disease of Cucurbits in California

Flock, R. A.; Mayhew, D. E.

doi:10.1094/pd-65-75

Cited by 50 publications

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“…The rapid and widespread appearance of CuLCV in cucurbit cultivation represents the most recent example of begomovirus emergence in the American Southwest and northern Mexico. Previous examples of begomovirus emergence in the region include SLCV-E and SLCV-R (11,16,27) in cucurbits, BCaMV in bean (8), PepGMV and Pepper huasteco yellow vein virus in peppers (39,43), and Chino del tomate virus (9) and Tomato leaf curl Sinaloa virus (22) in tomato. Emergent begomoviruses become noticed primarily due to the new economic consequences of the diseases they cause in cultivated crops and to altered host range or virulence.…”

Section: Discussionmentioning

confidence: 99%

“…Squash leaf curl (SLC) disease is problematic in Arizona (6,7,37), California (12,14,16,35), Texas (24), Sonora, Mexico (3,32,36), Guatemala (5), and Honduras (10). SLC disease was first observed in squash in California during 1977 to 1978 (16) and in cultivated buffalo gourd in Arizona at about the same time (37).…”

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confidence: 99%

“…SLC disease was first observed in squash in California during 1977 to 1978 (16) and in cultivated buffalo gourd in Arizona at about the same time (37). The virus complex associated with SLC disease was initially transmitted by the A biotype of Bemisia tabaci, which was native to the deserts of the southwestern United States and Sonora when the disease was first observed.…”

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confidence: 99%

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Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Brown¹,

Idris²,

Alteri³

et al. 2002

Phytopathology®

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Cucurbit leaf curl virus (CuLCV), a whitefly-transmitted geminivirus previously partially characterized from the southwestern United States and northern Mexico, was identified as a distinct bipartite begomovirus species. This virus has near sequence identity with the previously partially characterized Cucurbit leaf crumple virus from California. Experimental and natural host range studies indicated that CuLCV has a relatively broad host range within the family Cucurbitaceae and also infects bean and tobacco. The genome of an Arizona isolate, designated CuLCV-AZ, was cloned and completely sequenced. Cloned CuLCV-AZ DNA A and B components were infectious by biolistic inoculation to pumpkin and progeny virus was transmissible by the whitefly vector, Bemisia tabaci, thereby completing Koch's postulates. CuLCV-AZ DNA A shared highest nucleotide sequence identity with Squash leaf curl virus-R (SLCV-R), SLCV-E, and Bean calico mosaic virus (BCaMV) at 84, 83, and 80%, respectively. The CuLCV DNA B component shared highest nucleotide sequence identity with BCaMV, SLCV-R, and SLCV-E at 71, 70, and 68%, respectively. The cis-acting begomovirus replication specificity element, GGTGTCCTGGTG, in the CuLCV-AZ origin of replication is identical to that of SLCV-R, SLCV-E, and BCaMV, suggesting that reassortants among components of CuLCV-AZ and these begomoviruses may be possible. Reassortment experiments in pumpkin demonstrated that both reassortants of CuLCV-AZ and SLCV-E A and B components were viable. However, for CuLCV-AZ and SLCV-R, only one reassortant (SLCV-R DNA A/CuLCV-AZ DNA B) was viable on pumpkin, even though the cognate component pairs of both viruses infect pumpkin. These results demonstrate that reassortment among sympatric begomovirus species infecting cucurbits are possible, and that, if generated in nature, could result in begomoviruses bearing distinct biological properties.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Brown¹,

Idris²,

Alteri³

et al. 2002

Phytopathology®

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“…Squash leaf curl disease was first described in 1979 and 1980 in California (19) and Arizona (6,7,44) and was later shown to be caused by either a single begomovirus or a mixture of two, depending on the host. The viruses are recognized as the separate species SLCV and SMLCV (8,18), and evidence suggests that the two causal viruses have been present in the southwestern United States and northern Mexico since at least 1977.…”

Section: Squash Leaf Curl China Virusmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at ϳ90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

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“…Since then, the diseases caused by begomoviruses have been reported in bitter gourd, chayote, cucumber, muskmelon, pumpkin and sponge gourd [17,20,22,30,34]. Many different begomoviruses infecting cucurbits have been reported from other countries such as Squash leaf curl Yunnan virus from China [39], Melon chlorotic leaf curl virus from Guatemala [3] Chayote yellow mosaic virus from Nigeria [33], ToL-CNDV from Thailand [26,27], Watermelon chlorotic curly stunt virus from Sudan and Iran [13], Squash leaf curl Philippines virus from the Philippines [14], Cucurbit leaf curl virus [2], Cucurbit leaf crumple virus [9], Squash leaf curl virus [8,16] and Squash mild leaf curl virus [4] from USA, and Luffa yellow mosaic virus from Vietnam [24].…”

Section: Introductionmentioning

confidence: 99%

Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

et al. 2010

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Bottle gourd (Lagenaria siceraria) an important vegetable crop in India was observed to be affected by a chlorotic curly stunt disease (CCSD) during [2003][2004][2005][2006] in the vegetable growing areas of Delhi and adjoining state of Haryana. The affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. Incidence of the disease varied from 4.7 to 36%. The disease could be easily transmitted by whitefly, Bemisia tabaci but not by sap. The causal virus was found to be a Begomovirus on the basis of whitefly transmission and sequence identity of putative coat protein (CP) and replication initiator protein (Rep) genes. The virus was transmitted to Cucumis sativus, Luffa acutangula, L. cylndrica, Lycopersicon esculentum, Nicotiana tabacum and Praecitrullus fistulosus but not to Citrullus lunatus, Cucumis melo, Cucurbita moschata and Vigna unguiculata. The N-terminal 60 amino acids of CP of the virus had 100% sequence identity with all the isolates of Tomato leaf curl New Delhi virus (ToLCNDV) and two isolates of Squash leaf curl China virus (SLCCV). The full length amino acid sequence of the CP and Rep genes had 100% similarity with ToLCNDVSvr and -Luffa isolates. The phylogenetic analysis showed that the virus associated with CCSD of bottle gourd belongs to ToLCNDV cluster of the begomoviruses. This is the first record of emergence of a Begomovirus associated severe disease in bottle gourd in India.

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Squash Leaf Curl, a New Disease of Cucurbits in California

Cited by 50 publications

References 0 publications

Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

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