SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer

Abstract

Serine-rich splicing factor3 (SRSF3) plays an essential role in cell proliferation and inducing and maintaining of cancers as a proto-oncogene. However, the mechanisms of SRSF3 in pan-cancers are still unknown. In our study, a visualized prognostic landscape of SRSF3 in pan-cancer was investigated and the relationship between SRSF3 expression and immune infiltration was also investigated. The expression pattern and prognostic worth of SRSF3 among pan-cancers were explored through different databases, namely, the TCGA and Kaplan–Meier Plotter. Moreover, the survival analysis including Kaplan-Meier method for evaluating between groups was conducted. Further analyses including the correlation between expression SRSF expression and immune infiltration including tumor mutation burden (TMB), microsatellite instability (MSI) was investigated using Spearman test. In ACC, KIRP and UCEC cancer, upregulated expression of SRSF3 was associated with worse disease-free interval (DFI), representing a mechanism in promoting progression of tumor. Our results showed that SRSF3 expression was positively correlated immune cell infiltration, TMB, MSI in certain cancer types, indicating SRSF3 expression to potential value of therapy response. Additionally, we explored the functional characteristics of SRSF in vitro through western blot detecting the expression level of the apoptosis-related proteins in SW480 and 786-O cells. SRSF3 expression was upregulated in pan-cancer tissue compared with normal tissue, which confirmed by immunohistochemistry and its expression indicated poor overall survival and death-specific survival. Therefore, SRSF3 was found to be a possible biomarker for prognostic and therapeutic assessment through bioinformatic analysis. SRSF3 is expressed in various cancers and its high expression correlated to poor survival and disease progression. In summary, SRSF3 expression can be considered as a prognostic biomarker in pan-cancer and therapeutic evaluation.

Circular RNA hsa_circ_0050386 suppresses non-small cell lung cancer progression via regulating the SRSF3/FN1 axis

“…Li et al discovered that the expression of SRSF3 was higher in pan-cancer tissue in comparison to normal tissue. This overexpression suggested unfavorable outcomes for both overall survival and death-specific survival [32]. Jia et al discovered that SRSF3 controls alternative splicing of ILF3, thereby stimulating the growth and conversion of cancer cells [33].…”

Towards understandings of serine/arginine-rich splicing factors

“…Other researches also indicated involvements of aberrant SRSF activation in cancer-specific immune environment. In pan-cancer SRSF3 expression was positively correlated with immune cell infiltration, tumor mutation burden, microsatellite instability 169 . Dysfunction in T-cell antitumor activity resulting from high expression of programmed cell death-1 contributes to progression of clear cell renal carcinoma.…”