ß-Glucuronidase as a new potent biomarker in the diagnosis and prognosis of organophosphorus toxicity

Abstract

Background: Organophosphorus poisoning occurs as a result of accidental exposure, suicidal and homicidal attempts. Organophosphorus compounds are known to cause selective release of liver microsomal ß-glucuronidase (BG) into serum. Dissociation of the egasyn -ß-glucuronidase complex in the liver by organophosphorus compounds was followed by a massive release of ß-glucuronidase from liver into the serum. Objectives: There are few papers suggesting increase in activity of ß-glucuronidase (BG) in serum of experimental animals subjected to organophosphorus poisoning. But, still there is less or no such study in human beings having organophosphorus poisoning. Thus, aim of our this research was to estimate and correlate activity of ß-glucuronidase (BG) in different stages of organophosphorus poisoning. Methods: ß-glucuronidase (BG) activity was determined by Goldstein method. Results and Conclusions:We found that the serum activity of β -glucuronidase (BG) proportionally increases (p < 0.01) and correlates with severity of organophosphorus poisoning. Further, there was found a significant increase in β -glucuronidase (BG) activity in low dose (mild) poisoning also. Thus, β -glucuronidase (BG) can be used as a potent biochemical marker in the diagnosis and prognosis of organophosphorus poisoning along with acetylcholinesterase or may also without acetylcholinesterase.

Serum β-glucuronidase activity as a biomarker for acute cholinesterase inhibitor pesticide poisoning

“…This increase was found to occur 2–5 h after exposure then decreased to normal levels by 24 h (Kikuchi et al, 1981; Satoh et al, 1999). Other studies have been carried out in humans to examine the BG activity in acutely poisoned patients; they showed similarly a proportional increase in serum activity of BG and its correlation with the severity of OP poisoning (Dhotre et al, 2014; Kale, 2013).…”