SSR markers linked to mite (Polyphagotarsonemus latus Banks) resistance in jute (Corchorus olitorius L.)

Abstract

Abstract:The mite is considered as one of the major limiting factors of jute production in Bangladesh. So it is essential to select genotypes with the desired resistance allele combination using DNA markers. Eighty-eight SSR primers were used to screen the mite resistant and sensitive parents. Among them only twenty-one primer pairs showed polymorphism. A genetic linkage map was constructed using these polymorphic primers in one hundred and fifty F 2 populations, derived from a cross between the parents. The SSR markers J-170 and HK-89 have been mapped at 34.1 cM and 35.4 cM, respectively, on either side of the phenotypic marker. These two markers (J-170 and HK-89) along with HK-64 showed 100% selection efficiency in combination.

Genetic linkage map construction for white jute (Corchorus capsularis L.) using SRAP, ISSR and RAPD markers

“…, Ghosh et al. ) were successively developed. The use of these markers has been very popular because these marker methods are relatively easy to conduct and generate a relatively high number of DNA markers per unit assay.…”

“…, Ghosh et al. , Topdar et al. ), relatively few SSR markers are publicly available in white jute ( C. capsularis ).…”

“…Haque et al (2008) constructed a linkage map including 40 polymorphic randomly amplified polymorphic DNA (RAPD) markers in dark jute (C. olitorius). Recently, several genetic linkage maps were constructed using simple sequence repeat (SSR) markers (Das et al 2012 because the number of publicly available SSR markers in dark jute (C. olitorius) is increasing (Roy et al 2006, Mir et al 2007, Keka et al 2008, Huq et al 2009, Ghosh et al 2010. Das et al (2012) constructed a linkage map comprising six LGs covering 784.3 cM using 36 polymorphic SSRs in an RIL (recombinant inbred line) population from a cross between two C. olitorius genotypes (JRO524 and PPO4).…”

“…However, only a few studies involving the construction of linkage maps have been conducted in white jute (C. capsularis). With the invention of the polymerase chain reaction (PCR), a variety of DNA-based molecular markers such as RAPD (Takemori et al 1994, Reddy and Soliman 1997, Chen et al 2011), sequence-related amplified polymorphisms (SRAPs) Quiros 2001, Rana et al 2012), ISSRs (Zietkiewicz et al 1994, Chen et al 2011 and SSRs (Basu et al 2004, Roy et al 2006, Ghosh et al 2010 were successively developed. The use of these markers has been very popular because these marker methods are relatively easy to conduct and generate a relatively high number of DNA markers per unit assay.…”

“…To overcome the scarcity of linkage maps in white jute (C. capsularis), an abundance of DNA-based molecular markers, including RAPD, SRAPs, ISSRs and SSRs, should be utilized to construct linkage maps. However, compared with dark jute (C. olitorius) (Roy et al 2006, Ghosh et al 2010, relatively few SSR markers are publicly available in white jute (C. capsularis). In this study, SRAPs together with ISSRs and RAPD were utilized to construct a linkage map in white jute (C. capsularis).…”