SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

Ashkani, Sadegh; Rafii, Mohd Y.; Rusli, Ibrahim; Meon, Sariah; Abdullah, Siti Nor Akmar; Harun, Abdul Rahim; Latif, Abdul

doi:10.1007/s11105-011-0315-4

Cited by 62 publications

(49 citation statements)

References 34 publications

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“…The availability of molecular markers, along with marker-assisted selection (MAS) strategies, is essential for the development of rice varieties with durable blast resistance against different races of M. oryzae (Ashkani et al 2012). The pyramiding of three blast R genes, Pi1, Piz-5 and Pita-2, into cultivars doi: 10.17221/51/2016-CJGPB was undertaken to provide broad-spectrum resistance to many isolates of M. oryzae (Hittalmani et al 2000).…”

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confidence: 99%

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Suwannual¹,

Chankaew²,

Monkham³

et al. 2017

Czech J. Genet. Plant Breed.

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Suwannual T., Chankaew S., Monkham T., Saksirirat W., Sanitchon J. (2017): Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection. Czech J. Genet. Plant Breed., 53: 1-8.Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC 2 F 2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research.

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confidence: 99%

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Suwannual¹,

Chankaew²,

Monkham³

et al. 2017

Czech J. Genet. Plant Breed.

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“…In case of blast resistance, application of marker assisted selection is very powerful as single or a few genes are involved in the resistance mechanism. Development of durable blast resistant rice varieties against M. oryzae progressed with the availability of different molecular markers used for marker assisted selection (Ashkani et al, 2012).…”

Section: Mas and Gene Pyramiding For Blast Resistancementioning

confidence: 99%

Mapping and Deployment of Blast Resistance Gene in Rice – A Work in Progress

Debnath¹,

Bhutia²,

Sumpi³

2018

Int.J.Curr.Microbiol.App.Sci

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“…So up to now, rice cultivar with broad-spectrum of resistance to highly adaptive virulent isolates/races remains a challenge. In a MAS breeding for blast resistance, F3 population derived from the cross of Pongsu seribu 2 (resistant) and Mahsuri (susceptible) allowed the association of four SSR markers RM413, RM1233, RM8225, and RM5961 to blast resistant genes (Ashkani et al 2012). Because these markers had high selection accuracy for resistant plant sources, they were suggested to be used in MAS for the resistant gene.…”

Section: Blastmentioning

confidence: 99%

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)

Nogoy¹,

Song²,

Ouk³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Abiotic and biotic stresses adversely affect rice (Oryza sativa L.) growth and yield. Conventional breeding is a very effective method to develop tolerant rice variety; however, it takes a decade long to establish a new rice variety. DNA-based markers have a huge potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection (MAS). The large number of quantitative trait loci (QTLs) mapping studies for rice has provided an abundance of DNA marker-trait associations. The limitations of conventional breeding such as linkage drag and lengthy time consumption can be overcome by utilizing DNA markers in plant breeding. The major applications of DNA markers such as MAS, QTL mapping and gene pyramiding have been surveyed. In this review, we presented the latest markers available for some of the most important abiotic and biotic stresses in rice breeding programs. Achieving a significant impact on crop improvement by marker assisted breeding (MAB) represents the great challenge for agricultural scientists in the next few decades.

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SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

Cited by 62 publications

References 34 publications

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Mapping and Deployment of Blast Resistance Gene in Rice – A Work in Progress

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)

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