ST105 Deep sequencing reveals abundant Pol III retroviral microRNA cluster in Bovine Leukemia Virus-induced leukemia

Rosewick, Nicolas; Durkin, Keith; Momont, Mélanie; Takeda, Hitoshi; Caiment, Florian; Cleuter, Yvette; Vernin, Céline; Mortreux, Franck; Wattel, Éric; Burny, Arsène; Georges, Michel; Broeke, Anne Van den

doi:10.1097/01.qai.0000429267.82844.b6

Cited by 5 publications

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“…But advancement in cloning methodologies and computational algorithms resulted in a rapid increase in number of miRNAs identified in plants in last few years (Tripathi et al, 2015 ). In recent past, the advent of high throughput/deep sequencing has really helped in an exponential growth in the number of plant miRNAs, not only identified but also functionally annotated (Jagadeeswaran et al, 2010 ; Rosewick et al, 2013 ). This has resulted into establishment of biological databases to act as archives of miRNA-sequences and annotation such as miRBase (Tripathi et al, 2015 ).…”

Section: Identification and Target Prediction Of Plant Mirnas: Computmentioning

confidence: 99%

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

et al. 2016

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The microRNAs (miRNAs) are small (20–24 nt) sized, non-coding, single stranded riboregulator RNAs abundant in higher organisms. Recent findings have established that plants assign miRNAs as critical post-transcriptional regulators of gene expression in sequence-specific manner to respond to numerous abiotic stresses they face during their growth cycle. These small RNAs regulate gene expression via translational inhibition. Usually, stress induced miRNAs downregulate their target mRNAs, whereas, their downregulation leads to accumulation and function of positive regulators. In the past decade, investigations were mainly aimed to identify plant miRNAs, responsive to individual or multiple environmental factors, profiling their expression patterns and recognizing their roles in stress responses and tolerance. Altered expressions of miRNAs implicated in plant growth and development have been reported in several plant species subjected to abiotic stress conditions such as drought, salinity, extreme temperatures, nutrient deprivation, and heavy metals. These findings indicate that miRNAs may hold the key as potential targets for genetic manipulations to engineer abiotic stress tolerance in crop plants. This review is aimed to provide recent updates on plant miRNAs, their biogenesis and functions, target prediction and identification, computational tools and databases available for plant miRNAs, and their roles in abiotic stress-responses and adaptive mechanisms in major crop plants. Besides, the recent case studies for overexpressing the selected miRNAs for miRNA-mediated enhanced abiotic stress tolerance of transgenic plants have been discussed.

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Section: Identification and Target Prediction Of Plant Mirnas: Computmentioning

confidence: 99%

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

et al. 2016

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“…Coupled with nextgeneration sequencing, improved algorithm has made it possible. This rapid increase is also due to the advancement in cloning techniques and analysis methods (Jagadeeswaran et al 2010;Rosewick et al 2013). Further, utilization of different bioinformatics databases and tools have revolutionized the study of miRNAs and their regulatory roles during different abiotic stresses including HS.…”

Section: Role Of Bioinformatics In Establishing Mirna and Plant Hs Tolerance Interactionsmentioning

confidence: 99%

miRNomes involved in imparting thermotolerance to crop plants

2018

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Thermal stress is one of the challenges to crop plants that negatively impacts crop yield. To overcome this ever-growing problem, utilization of regulatory mechanisms, especially microRNAs (miRNAs), that provide efficient and precise regulation in a targeted manner have been found to play determining roles. Besides their roles in plant growth and development, many recent studies have shown differential regulation of several miRNAs during abiotic stresses including heat stress (HS). Thus, understanding the underlying mechanism of miRNA-mediated gene expression during HS will enable researchers to exploit this regulatory mechanism to address HS responses. This review focuses on the miRNAs and regulatory networks that were involved in physiological, metabolic and morphological adaptations during HS in plant, specifically in crops. Illustrated examples including, the miR156-SPL, miR169-NF-YA5, miR395-APS/AST, miR396-WRKY, etc., have been discussed in specific relation to the crop plants. Further, we have also discussed the available plant miRNA databases and bioinformatics tools useful for miRNA identification and study of their regulatory role in response to HS. Finally, we have briefly discussed the future prospects about the miRNA-related mechanisms of HS for improving thermotolerance in crop plants.

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“…The numbers however increased rapidly with the advancement in cloning techniques and computational algorithms. In the past few years high throughput sequencing and screening protocols has caused an exponential increase in number of miRs, identified and functionally annotated from various plant species (Rajagopalan et al, 2006 ; Fahlgren et al, 2007 ; Jagadeeswaran et al, 2010 ; Rosewick et al, 2013 ). This is best exemplified by the establishment of miRBase, a biological database that acts as an archive of miR sequences and annotations (Griffiths-Jones, 2004 ; Griffiths-Jones et al, 2008 ; Kozomara and Griffiths-Jones, 2014 ).…”

Section: Identification Of Stress-associated Micrornasmentioning

confidence: 99%

Role of bioinformatics in establishing microRNAs as modulators of abiotic stress responses: the new revolution

2015

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microRNAs (miRs) are a class of 21–24 nucleotide long non-coding RNAs responsible for regulating the expression of associated genes mainly by cleavage or translational inhibition of the target transcripts. With this characteristic of silencing, miRs act as an important component in regulation of plant responses in various stress conditions. In recent years, with drastic change in environmental and soil conditions different type of stresses have emerged as a major challenge for plants growth and productivity. The identification and profiling of miRs has itself been a challenge for research workers given their small size and large number of many probable sequences in the genome. Application of computational approaches has expedited the process of identification of miRs and their expression profiling in different conditions. The development of High-Throughput Sequencing (HTS) techniques has facilitated to gain access to the global profiles of the miRs for understanding their mode of action in plants. Introduction of various bioinformatics databases and tools have revolutionized the study of miRs and other small RNAs. This review focuses the role of bioinformatics approaches in the identification and study of the regulatory roles of plant miRs in the adaptive response to stresses.

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ST105 Deep sequencing reveals abundant Pol III retroviral microRNA cluster in Bovine Leukemia Virus-induced leukemia

Cited by 5 publications

References 0 publications

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

miRNomes involved in imparting thermotolerance to crop plants

Role of bioinformatics in establishing microRNAs as modulators of abiotic stress responses: the new revolution

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