StABI5 Involved in the Regulation of Chloroplast Development and Photosynthesis in Potato

Abstract

Abscisic acid (ABA) insensitive 5 (ABI5)—a core transcription factor of the ABA signaling pathway—is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth. ABI5 interacts with other phytohormone signals to regulate plant growth and development, and stress responses in Arabidopsis, but little is known about the functions of ABI5 in potatoes. Here, we find that StABI5 is involved in the regulation of chloroplast development and photosynthesis. Genetic analysis indicates that StABI5 overexpression transgenic potato lines accelerate dark-induced leaf yellowing and senescence. The chlorophyll contents of overexpressed StABI5 transgenic potato lines were significantly decreased in comparison to those of wild-type Desiree potatoes under dark conditions. Additionally, the RNA-sequencing (RNA-seq) analysis shows that many metabolic processes are changed in overexpressed StABI5 transgenic potatoes. Most of the genes involved in photosynthesis and carbon fixation are significantly down-regulated, especially the chlorophyll a-b binding protein, photosystem I, and photosystem II. These observations indicate that StABI5 negatively regulates chloroplast development and photosynthesis, and provides some insights into the functions of StABI5 in regard to potato growth.

Phytohormones: plant switchers in developmental and growth stages in potato

“…In potato, StABI5 plays an important role in the regulation of tuber dormancy through the controlling of the auxin signaling pathway. In addition, StABI5 regulates the genes (i.e., SUAR and AUX) downstream of the auxin signaling [ 24 ].…”

ABSCISIC ACID INSENSITIVE5 Interacts With RIBOSOMAL S6 KINASE2 to Mediate ABA Responses During Seedling Growth in Arabidopsis

“…Phytohormone ABA plays key roles in vegetative, seed dormant and germination, and biotic and abiotic stresses such as drought, salt stress, and pathogen infection ( Jurriaan et al, 2009 ; Zhao et al, 2016 ; Vishwakarma et al, 2017 ; Kumar et al, 2019 ). ABI5 is a key transcription factor in regulating the expression of ABA-responsive genes in the ABA signaling pathway ( Skubacz et al, 2016 ; Zhu et al, 2020 ). Previous study revealed that ABI5 was phosphorylated at Thr-35, Ser-36, Ser-41, Ser-42, Ser-138, Ser-139, Ser-145, and Thr-201 residues in Arabidopsis ( Hu and Yu, 2014 ; Yu et al, 2015 ), of which Ser-42, Ser-145, and Thr-201 sites of ABI5 were phosphorylated by SnRK2s protein kinases ( Fujii and Zhu, 2009 ).…”

The Overexpression of The VvWRKY2 Transcription Factor in Potato Improved the Agricultural Performance and Tubers’ Physio-Chemical and Industrial Properties Even Under Non‐Stress Conditions

“…Potato is an important food crop in the world, occupying the fourth rank after wheat, rice, and corn (Zhu et al 2020). However, because of its high sensitivity, biotic and abiotic constraints limit the productivity of this plant (Chacón-Cerdas et al 2020).…”