Stabilization of <i>Bombyx mori</i> silk fibroin/sericin films by crosslinking with PEG-DE 600 and genipin

Motta, Antonella; Barbato, B; Foss, Cristina; Torricelli, Paola; Migliaresi, Claudio

doi:10.1177/0883911511400251

Cited by 43 publications

(53 citation statements)

References 43 publications

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“…6 C, this behavior was due to b-sheet structure. 42,43 In addition, BSF/TSF blend lms appeared two endothermic peaks at 278. 6 C and 360.6 C, which belonged to endothermic peaks of BSF and TSF, respectively.…”

Section: View Article Onlinementioning

confidence: 99%

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

Yang

Wang

et al. 2018

RSC Adv.

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The possibility of using silk fibroin in biomaterials for tissue engineering is a subject of broad interest. In this study, Bombyx mori/tussah silk fibroin (BSF/TSF) blend films were prepared by solution casting using CaCl 2 / formic acid as a co-solvent and water as a rinse solvent. The morphology, crystallinity, thermal resistance, mechanical properties and water contact angle of the blend films as well as the biocompatibility were investigated. The BSF/TSF blend films displayed a smooth surface and specific nanostructure in their cross-section, originating from the nanofibril-preservation during fibroin dissolution. The water rinse process induced the formation of a stable b-sheet structure. The BSF film showed superior mechanical properties to the TSF film, and the blending with TSF led to a significant reduction in the strength and elasticity of blend films. However, adding the TSF component could regulate the hydrophilic properties and enhance cell growth on the blend films. The BSF/TSF blend films with specific nanostructure, stable secondary structure, appropriate mechanical properties, and good biocompatibility, are promising candidates for application in regenerative medicine.

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Section: View Article Onlinementioning

confidence: 99%

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

Yang

Wang

et al. 2018

RSC Adv.

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“…Addition of the isocyanate compounds to sericin films exhibits lower solubility and also lower swell ratio to the distilled water, indicating that sericin films could retain more biodegradability. Crosslinked fibroin/sericin 90/10 films fabricated using two different crosslinkers poly(ethylene glycol) diglycidyl ether 600 (PEG-DE) and genipin, exhibit good biocompatibility and support cell adhesion and proliferation [47]. Teramoto et al showed that the sericin gel film rapidly absorbs water and equilibrates at a content of about 80% without cytotoxicity [15].…”

Section: (B) Filmsmentioning

confidence: 99%

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

Nayak

Kundu

2016

Biomaterials From Nature for Advanced Devices and Therapies

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“…Motta et al [24] used PEG-DE to cross-link fibroin and sericin and indicated that PEG-DE stabilized the random/α-helix structures in the fi broin and prevented phase separation between fi broin and sericin, and the cross-linked fi lms exhibited good biocompatibility. Antheraea pernyi silk fibroin was reported to have no changes in molecular conformations caused by PEG-DE in the regenerated film at 40 ℃, and the crosslinked regenerated film showed remarkable compliance and tenacity [26] .…”

Section: Introductionmentioning

confidence: 98%

“…The molecular conformation of regenerated SF material can be translated from random coil structures into β-sheets (Silk II) or β-turns induced by genipin, to obtain water-insoluble SF materials [23] . The genipin crosslinked silk fi lm displayed highest elongation at break and tensile strength [24] . In addition, metal ions such as cupric ion can induce the SF secondary structure transition from an amorphous to a β-sheet structure [25] .…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of PEGDE crosslinked silk fibroin film

Wei

Sun

Huang

et al. 2014

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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To obtain water-insoluble silk fibroin (SF) materials, polyethylene glycol diglycidyl ether (PEG-DE) was selected as a crosslinking agent to prepare SF films (blends). The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF:PEG-DE was 1.0:0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form β-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend fi lms. In order to demonstrate the cytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend fi lm (SF:PEG-DE = 1:1) and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend fi lm resulting in high cell viability. The extracted liquid from the SF porous fi lm did not inhibit cell proliferation, as estimated by the MTT assay.

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Stabilization of Bombyx mori silk fibroin/sericin films by crosslinking with PEG-DE 600 and genipin

Cited by 43 publications

References 43 publications

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

A novel method to prepare tussah/Bombyx mori silk fibroin-based films

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

Preparation and characterization of PEGDE crosslinked silk fibroin film

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