Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

Nikolelis, Dimitrios P.; Raftopoulou, Garyfallia; Nikoleli, Georgia‐Paraskevi; Simantiraki, Maria

doi:10.1002/elan.200603702

Cited by 46 publications

(80 citation statements)

References 24 publications

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“…Stabilized lipid films were prepared by polymerization that was previously described in the literature [12,20,21,23,24]. The polymerization took place by using UV irradiation instead of the thermal polymerization; 5 mg of DPPC were mixed with 0.070 mL of methacrylic acid, 0.8 mL of ethylene glycol dimethacrylate, 8 mg of 2,2'-azobis-(2-methylpropionitrile), 1.0 mL of acetonitrile and 0.29 mg of the phosphoryl receptor.…”

Section: Methodsmentioning

confidence: 99%

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Development of an Electrochemical Biosensor for the Rapid Detection of Carbofuran Based on Air Stable Lipid Films with Incorporated Calix[4]arene Phosphoryl Receptor

et al. 2008

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This work describes the preparation of a selective receptor for the rapid, selective and sensitive electrochemical flow injection analysis of carbofuran in foods using air stable lipid films supported on a methacrylate polymer on a glass fiber filter with incorporated artificial receptor. The selective receptor was synthesized by transformation of the ÀOH groups of resorcin [4]arene receptor into phosphoryl groups. These lipid films were supported on a methylacrylate polymer (i.e., methacrylic acid was the functional monomer for the polymerization, ethylene glycol dimethacrylate was used as the crosslinker and 2,2'-azobis-2-methylpropionitrile as an initiator). A minisensor device was constructed for the electrochemical flow injection analysis of toxicants based on air stabilized lipid films supported on a polymer. The device can sense the analyte in a drop (50 mL) of sample. Carbofuran was injected into flowing streams of a carrier electrolyte solution. A host-guest complex formation between the calix[4]arene phosphoryl receptor and carbofuran takes place through hydrogen bonding. This enhances the preconcentration of carbofuran at the lipid membrane surface which in turn causes dynamic alterations of the electrostatic fields and phase structure of membranes; as a result ion current transients were obtained and the magnitude of these signals was correlated to the substrate concentration. The response times were ca. 80 s and carbofuran was determined at concentration levels of nM. The effect of potent interferences included a wide range of compounds and other insecticides. The effect of interference of proteins and lipids was also examined. The reproducibility of the method was checked by recovery experiments in fruit and vegetable samples with satisfactory results.

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Section: Methodsmentioning

confidence: 99%

“…The filter with the mixture was then irradiated using the UV deuterium lamp. Raman spectrometry was used to monitor the kinetics of the process of polymerization [24]. The polymerization was completed within 4 hours.…”

Section: Methodsmentioning

confidence: 99%

Development of an Electrochemical Biosensor for the Rapid Detection of Carbofuran Based on Air Stable Lipid Films with Incorporated Calix[4]arene Phosphoryl Receptor

et al. 2008

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“…The formation of stabilized lipid film was previously described [27,30,31]. A complete description of the experimental setup used was described previously in literature [27,30,31].…”

Section: Apparatusmentioning

confidence: 99%

“…A complete description of the experimental setup used was described previously in literature [27,30,31]. One of the Ag/AgCl reference electrodes was immersed in the waste of the carrier electrolyte solution, whereas the other Ag/AgCl reference electrode was placed into the cylindrical cell.…”

Section: Apparatusmentioning

confidence: 99%

Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti‐PCB Antibody

et al. 2011

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This work describes a novel electrochemical biosensor based on a supported polymerized lipid film with incorporated Sheep anti-PCB antibody for the rapid detection of arochlor 1242 in flowing solution streams. The antibody was incorporated into the lipid film during polymerization. Injections of antigen were made into flowing streams of a carrier electrolyte solution. Experiments were done in a stopped-flow mode using lipid mixtures containing 15 % (w/w) dipalmitoylphosphatidic acid (DPPA) to provide only a single transient current signal with a magnitude related to the antigen concentration. Lipid films containing 35 % DPPA were used to examine regeneration of the active sites of antibody after complex formation by washing with the carrier electrolyte solution. Repetitive cycles of injection of antigen have shown that the maximum number of cycles is about 5.

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“…The construction of the graphene electrode has been described in detail in one of our previous papers [17]; a 0.4 mg/mL homogeneous graphene dispersion had been obtained in N-methyl-pyrrolidone (NMP) after mild sonication (using a Bandelin SONOREX Digital 10P sonicator, Sigma-Aldrich, Taufkirchen Germany) for 180 h and centrifugation at 700 rpm for 2 h. Stabilized lipid films were prepared by polymerization, as previously described in the literature [18,19]. Shortly, 5 mg of DPPC were added to a mixture of 0.070 mL of methylacrylic acid, 0.8 mL of ethylene glycol dimethacrylate, 8 mg of 2,2 -azobis-(2-methylpropionitrile), 1.0 mL of acetonitrile.…”

Section: Fabrication Of the Lipid-based Sensor Electrode With Incorpomentioning

confidence: 99%

Biosensors Based on Lipid Modified Graphene Microelectrodes

Nikoleli

Siontorou

Nikolelis

et al. 2017

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Graphene is one of the new materials which has shown a large impact on the electronic industry due to its versatile properties, such as high specific surface area, high electrical conductivity, chemical stability, and large spectrum of electrochemical properties. The graphene material-based electronic industry has provided flexible devices which are inexpensive, simple and low power-consuming sensor tools, therefore opening an outstanding new door in the field of portable electronic devices. All these attractive advantages of graphene give a platform for the development of a new generation of devices in both food and environmental applications. Lipid-based sensors have proven to be a good route to the construction of novel devices with improved characteristics, such as fast response times, increased sensitivity and selectivity, and the possibility of miniaturization for the construction of portable biosensors. Therefore, the incorporation of a lipid substrate on graphene electrodes has provided a route to the construction of a highly sensitive and selective class of biosensors with fast response times and portability of field applications for the rapid detection of toxicants in the environment and food products.

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Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses

Cited by 46 publications

References 24 publications

Development of an Electrochemical Biosensor for the Rapid Detection of Carbofuran Based on Air Stable Lipid Films with Incorporated Calix[4]arene Phosphoryl Receptor

Development of an Electrochemical Biosensor for the Rapid Detection of Carbofuran Based on Air Stable Lipid Films with Incorporated Calix[4]arene Phosphoryl Receptor

Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti‐PCB Antibody

Biosensors Based on Lipid Modified Graphene Microelectrodes

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