Stable isotope labeling derivatization coupled with magnetic dispersive solid phase extraction for the determination of hydroxyl-containing cholesterol and metabolites by in vivo microdialysis and ultra-high performance liquid chromatography tandem mass spectrometry

Wang, Xin; Sun, Jing; Xu, Yanqiu; Sun, Luping; Zhu, Shuyun; You, Jing; Wang, Xiao

doi:10.1016/j.chroma.2019.02.021

Cited by 22 publications

(12 citation statements)

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“…Fifteen biomarkers (10 of them are potential biomarkers found in nontargeted metabolomics) were quantitatively analyzed using multiple reaction monitoring. Multiple reaction monitoring detection was a sensitive, accurate, rapid, and specific quantification of multianalytes in a short chromatographic separation [19,20].…”

Section: Methodsmentioning

confidence: 99%

A metabolomic study of the urine of rats with Alzheimer's disease and the efficacy of Ding‐Zhi‐Xiao‐Wan on the afflicted rats

Wang

Liu

et al. 2020

J of Separation Science

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As a well-known traditional Chinese medicine formula, Ding-Zhi-Xiao-Wan has long been used for the routine treatment of Alzheimer's disease. However, the mechanism of Ding-Zhi-Xiao-Wan in treating Alzheimer's disease is unclear. Therefore, a nontargeted metabolomics method based on ultrahigh performance liquid chromatography with quadrupole time-of-flight mass spectrometry has been established to explore the metabolic variations in the urine of Alzheimer's disease rats and investigate the therapeutic mechanism of Ding-Zhi-Xiao-Wan on Alzheimer's disease. To develop a better rat model of Alzheimer's disease, amyloid β25-35 was injected into the bilateral hippocampus of Sprague-Dawley rats. Multivariate analysis approaches were applied to differentiate the urine components between the four groups. Thereafter, a targeted metabolomics method was used to verify the identified endogenous metabolites and determine the mechanism of action of Ding-Zhi-Xiao-Wan. Altogether, 26 potential biomarkers were found, of which 15 biomarkers (10 of which are potential biomarkers found in nontargeted metabolomics) were identified. The results show that Ding-Zhi-Xiao-Wan mainly affects the pathways of taurine and hypotaurine metabolism, tryptophan metabolism, and phenylalanine metabolism. Ding-Zhi-Xiao-Wan might play a role in the treatment of Alzheimer's disease by mediating antioxidative stress, regulation of energy metabolism, improvement of intestinal microbes, and protection of nerve cells.

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Section: Methodsmentioning

confidence: 99%

A metabolomic study of the urine of rats with Alzheimer's disease and the efficacy of Ding‐Zhi‐Xiao‐Wan on the afflicted rats

Wang

Liu

et al. 2020

J of Separation Science

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“…It was applied for the simultaneous and dynamic measurement of multiple neurosteroids in normal and Parkinson's disease rat blood microdialysates. Using the same SILD reagents, we developed an accurate, sensitive, and rapid method for the determination of hydroxyl-containing cholesterol and metabolites in rat blood microdialysates by microwave-assisted SILD magnetic dispersive SPE coupled with UHPLC-ESI-MS/MS in MRM mode [58]. This work can provide technical support in the diagnosis and treatment of Alzheimer's disease because hydroxyl-containing cholesterol and related metabolites are potential biomarkers for Alzheimer's disease.…”

Section: Amino-carbonyl Reaction or Esterification Derivatization Appmentioning

confidence: 99%

“…Recently, based on the advantage of rhodamine derivatization reagent structure for MS and MS/MS performance in positive ion mode [38,[52][53][54][55][56][57][58], the authors used a conventional amine-reactive fluorescent probe Nhydroxysuccinimidyl rhodamine B ester [S25] as derivatization reagent to label a classical phosphatidylethanolamine (PE 16:0 18:1) to evaluate its analytical performance of UHPLC-MS/MS (MRM). The results indicated that the LOD of this derivatization method was lower about two to three orders of magnitude than the nonderivatization method.…”

Section: Amino-carbonyl Reaction or Esterification Derivatization Appmentioning

confidence: 99%

Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry

Zhao

Zhu

Liu

2020

J of Separation Science

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Lipidomics plays an essential role in the development of an improved understanding of lipids metabolism and the identification of new biomarkers or therapeutic targets of related diseases. The strong analytical power of mass spectrometry and its rapid developments in the respect of instruments and techniques have significantly accelerated the emerging lipidomics and related application fields in biology, medicine, and pharmacy. The strategy of chemical derivatization can remarkably improve the shortcomings of mass spectrometric analytical technologies of shotgun lipidomics and liquid chromatography mass spectrometry, and in the past decade many related studies have been reported for fatty acids, glycerophospholipids, sphingomyelins, monoglycerides, diacylglycerols, long-chain bases, steroids, and so on. Therefore, this review will focus on new chemical derivatization approaches about the research progresses of shotgunbased and liquid chromatography mass spectrometry-based targeted lipidomics (from 2005 to July 2019, most of reports emerged in the past 5 years), and put forward the problems and prospects in this field. It is expected to be helpful for the design and synthesis of new derivatization reagents, especially the outstanding stable isotope labeling derivatization reagents, and the development and application of new chemical derivatization strategies and matched mass spectrometric analysis methods. K E Y W O R D Schemical derivatization, lipidomics, liquid chromatography mass spectrometry, shotgun lipidomics, stable isotope labeling 1838

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“…As mentioned previously, 2 H 4 ‐DMBA was also used as a substrate for the synthesis of other ICD reagents including 2 H 4 ‐DAPPz and 2 H 4 ‐DAPAP, which target carboxylic acids (Ogawa et al, ; Ogawa, Tadokoro, et al, ). Recently, the 2 H 0 / 2 H 3 ‐3‐ N ‐methyl‐2′‐carboxyl rhodamine 6G ( 2 H 0 / 2 H 3 ‐MCR6G) pair was used for derivatization of hydroxyl‐containing cholesterol and metabolites as indicators for Alzheimer's disease (Wang, Sun, et al, ). In addition, this pair of reagents were also applied for the determination of eight hydroxyl‐containing neurosteroids in rat blood microdialysates as indicators for Parkinson's disease (Xu et al, ).…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 99%

“…The derivatization reaction was carried out at 55 or 70°C under microwave irradiation. The samples were analyzed by UPLC–MS/MS showing good accuracy, linearity, and minimum matrix effects (Wang, Sun, et al, ; Xu et al, ). Lastly, the 18 O 0 / 18 O 2 ‐TSPC reagent pair could be used for chiral derivatization of hydroxyl and amine immunometabolite of 2‐hydroxyglutarate Zheng et al (), as mentioned previously in our discussion about ICD reagents targeting amines (see Table ).…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

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Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

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Stable isotope labeling derivatization coupled with magnetic dispersive solid phase extraction for the determination of hydroxyl-containing cholesterol and metabolites by in vivo microdialysis and ultra-high performance liquid chromatography tandem mass spectrometry

Cited by 22 publications

References 50 publications

A metabolomic study of the urine of rats with Alzheimer's disease and the efficacy of Ding‐Zhi‐Xiao‐Wan on the afflicted rats

A metabolomic study of the urine of rats with Alzheimer's disease and the efficacy of Ding‐Zhi‐Xiao‐Wan on the afflicted rats

Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

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