Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation

Abstract

Abstract:The synthesis of polymer brush decorated silica nano-particles is demonstrated by activator regeneration by electron transfer atom transfer radical polymerization (ARGET ATRP) grafting of poly(tert-butyl methacrylate). ATRP initiator decorated silica nano-particles were obtained using a novel trimethylsiloxane derivatised ATRP initiator obtained by click chemistry. Comparison of de-grafted polymers with polymer obtained from a sacrificial initiator demonstrated good agreement up to 55% monomer conversion. Subsequent mild deprotection of the tert-butyl ester groups using phosphoric acid yielded highly colloidal and pH stable hydrophilic nano-particles comprising approximately 50% methacrylic acid groups. The successful bio-conjugation was achieved by immobilization of Horseradish Peroxidase to the polymer brush decorated nano-particles and the enzyme activity demonstrated in a conversion of o-phenylene diamine dihydrochloride assay.

Polymer brush decorated nanoparticles immobilised on polymer monoliths for enhanced biopolymer elution

“…2. 46,47 This resulted in a 20 nm polymer brush layer determined by Dynamic Light Scattering (DLS see Fig. S3, † SEM image see Fig.…”

“…45 The polymer brushes thus comprise a copolymer structure poly(tert-butyl acrylate-co-acrylic acid) but it is assumed that unprotected acrylic acid is only present close to the silica core as the particles were hydrophilic and fully suspendable in water (denoted as PMA-SiNP). 47 The steps involved in the decoration of the monolith with the PMA-SiNP are depicted in Fig. 2B.…”

One‐step Synthesis of End‐Functionalized Hydrogenated Nitrile‐Butadiene Rubber by Combining the Functional Metathesis with Hydrogenation

“…[1][2][3][4][5][6][7] In recent years, the olefin metathesis reaction has received widespread attention for the synthesis of rubber due to its mild conditions, environment friendliness, high efficiency, and controllable performance. [8][9][10][11] The olefin metathesis reaction can be used for the chemical blending between unsaturated rubber and for the degradation of olefin unsaturated polymers, [12][13][14] such as polybutadiene (PBD), polyisoprene (PIp), [15] and poly(styrene-co-butadiene) (SBR). With the further development of olefin metathesis, adding different types of functional olefins in the olefin metathesis reaction sequence can conveniently introduce ester, [16] carboxyl hydroxyl, [17] and halogen groups at the end of the rubber molecular chain to form polymer materials with specific functions.…”

Silica Modified by Using Alcohol Polyoxyethylene Ether and Silane Coupling Agent together to Achieve High Performance Rubber Composites Using Latex Compounding Method

“…As an inorganic particle, silica has huge amounts of hydroxyl groups (-OH) on its surface [7], leading to the nature of the silica particle [8][9][10]. Therefore, silica particle is less compatible with a hydrophobic polymer, such as rubber [11].…”