Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis

Yu, Kunqian; Wei, Chong Kian; Hou, Zhanwu; Wang, Zilong; Yuan, Jiaqiang; Jiang, Yu; Zhao, Yongxi; Tang, Yawen; Gao, Meili

doi:10.1155/2014/212697

Cited by 12 publications

(3 citation statements)

References 25 publications

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“…To date, various analytical techniques have been applied for the determination of melamine in milk products, which mainly rely on chromatographic [ 6 , 7 ], spectroscopic [ 8 , 9 ] and other separation methods, such as ELISA [ 10 ] and capillary electrophoresis [ 11 ]. These methods are well known for their high sensitivity and accuracy.…”

Section: Introductionmentioning

confidence: 99%

Visual and Optical Absorbance Detection of Melamine in Milk by Melamine-Induced Aggregation of Gold Nanoparticles

et al. 2021

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The present study reported a facile method for the determination of melamine in milk powder products based on the aggregation of reactant-free 5 nm gold nanoparticles (AuNPs). The strong electrostatic attraction between the positively charged exocyclic amine groups present in the melamine molecule and the negatively charged ions bound to the AuNPs induced aggregation of the AuNPs, resulting in visible color changes that could be seen with the naked eye and monitored by ultraviolet–visible (UV–Vis) absorbance spectra. The method shows high sensitivity with detection limits of 1 × 10−9 M for visual detection and 1 × 10−11 M for UV–Vis analysis, which is far below the safety limit of melamine ingestion in infant formula (1 ppm = 7.9 × 10−6 M) and the detection limit acquired by most AuNP-based melamine detection methods. Good recoveries were obtained over the range of 94.7–95.5% with a relative standard deviation of mean recovery (RSD) ranging from 1.40 to 5.81. The method provides a simple, feasible, fast and real-time detection of melamine adulterants in infant formula by the naked eye, without the aid of advanced instruments.

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Section: Introductionmentioning

confidence: 99%

Visual and Optical Absorbance Detection of Melamine in Milk by Melamine-Induced Aggregation of Gold Nanoparticles

et al. 2021

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“…However, there were relatively few methods on the residue analyses of CYR and MEL in complex samples, most include photometric analysis, flow injection, high performance liquid chromatography (HPLC) and gas chromatography . However, owing to its high separation efficiency, low sample consumption and low analysis cost, capillary electrophoresis (CE) has been applied for the simultaneous determination of CYR and MEL in milk, chicken and other foods based on ultraviolet, diode array detector, electrogenerated chemiluminescence, and amperometric detection . Capacitively coupled contactless conductivity detection (C 4 D) has been recently developed as a universal detection technology for electrophoretic analysis since it can determine the charged substances without derivatization, and effectively avoid electrode contamination .…”

Section: Introductionmentioning

confidence: 99%

“…Solvent extraction, solid phase extraction with different cartridges and dispersive solid phase extraction have been used for sample purification and extraction, however, the preprocessing procedures were usually complicated and involved evaporation and redissolution of the eluent, as well as high solvent consumption. Electrophoretic stacking based on acetonitrile was also used for online enrichment of the target analytes in milk products . Furthermore, two‐phase hollow‐fiber liquid‐phase microextraction (HF‐LPME) has been applied for preconcentration of MEL in soil and milk samples due to its favorable sample purification and anti‐interference abilities as well as low organic solvent consumption.…”

Section: Introductionmentioning

confidence: 99%

Double surfactants‐assisted electromembrane extraction of cyromazine and melamine in surface water, soil and cucumber samples followed by capillary electrophoresis with contactless conductivity detection

et al. 2019

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BACKGROUND: Cyromazine (CYR) and its main degradation product melamine (MEL) are attracting wide attention due to their potential hazards to the environment and humans. In this work, double surfactants-assisted electromembrane extraction (DS-EME) by Tween 20 and alkylated phosphate was firstly used for purification and extraction of CYR and MEL, and the extract was directly analyzed by capillary electrophoresis with capacitively coupled contactless conductivity detection. RESULTS: Under the optimum conditions, two targets could be well separated from the main interferences, including common biogenic amines and inorganic cationswithin 14 min. This developed method was successfully applied to the analyses of surface water, soil and cucumber samples, and the average recoveries were in the range 93.3-112%. DS-EME provided a synergistic purification and enrichment effect for CYR and MEL by adding Tween 20 and alkylated phosphate into donor phase and supporting liquid membrane, respectively. Satisfactory limits of detection [0.2-1.5 ng mL −1 , signal-to-noise ratio (S/N) = 3] could be obtained in the tested sample matrices, and the corresponding enrichment factors were up to 115∼123 times. CONCLUSION: This developed method provides an alternative for the simultaneous analysis of CYR and MEL in complex real-world samples.Figure 2. Effects of the pH values in (A) donor phase (DP) and (B) accept phase (AP) on the enrichment factors (EFs) of target analytes. Enrichment conditions: supporting liquid membrane (SLM), 80% NPOE + 10% DEHP + 10% TEHP; and other conditions are the same as those in Fig. 1.

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Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody

et al. 2015

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A sensitive class-specific monoclonal antibody against tetracyclines (TCs) was generated and used to develop an enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic assay for TC, oxytetracycline (OTC), and chlortetracycline (CTC) detection in milk and honey samples. The dynamic range of detection for TC in ELISA was 0.26-2.00 μg L −1 with an IC 50 of 0.72 μg L −1 . The IC 50 value of OTC and CTC was 3.2 and 6.4 μg L −1 , respectively. The recovery of TC, OTC, and CTC in milk samples was 82-102, 91-105, and 90-101 %, respectively, and 88-101, 89-101, and 89-95 % in honey samples, respectively. In the immunochromatographic assay, the cutoff values for TC, OTC, and CTC were 15, 15, and 50 μg L −1 in milk, respectively, and 40, 40, and 40 μg L −1 in honey, respectively. The results revealed that ELISA and the immunochromatographic assay can be applied for the rapid and sensitive detection of TC, OTC, and CTC in milk and honey samples.

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Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis

Cited by 12 publications

References 25 publications

Visual and Optical Absorbance Detection of Melamine in Milk by Melamine-Induced Aggregation of Gold Nanoparticles

Visual and Optical Absorbance Detection of Melamine in Milk by Melamine-Induced Aggregation of Gold Nanoparticles

Double surfactants‐assisted electromembrane extraction of cyromazine and melamine in surface water, soil and cucumber samples followed by capillary electrophoresis with contactless conductivity detection

Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody

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