Stage and tissue-specific expression of a collagen gene during Drosophila melanogaster development

Parco, Yannick Le; Knibiehler, Bernard; Cecchini, Jean Pierre; Mirre, C.

doi:10.1016/0014-4827(86)90071-6

Cited by 33 publications

(18 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously demonstrated (Le Parco et al, 1986bParco et al, , 1989Knibiehler et al, 1987Knibiehler et al, , 1990Mirre et al, 1988), DCgl gene was expressed in tissues of mesodermal origin and the collagen IV produced was deposited on tissues that did not express it. The main sites of expression were circulating mesenchymal cells during embryogenesis and metamorphosis on one hand, and fat-bodies during phases of larval growth on the other.…”

Section: Discussionsupporting

confidence: 65%

“…During the first, second, and the beginning of the third larval stage, when larval CNS only increases in volume without any gross morphological modification (Power, 1952), the main sites of DCgl mRNA expression were the fat-bodies and some specialized tissues of mesodermal origin (Le Parco et al, 1986b;, but no specific signal was observed on neuronal tissues. DCgl was not expressed within the CNS, even at the end of third larval stage, when metamorphosis begins.…”

Section: Collagen IV Expression During the Formation Of The Imaginal Cnsmentioning

confidence: 99%

See 1 more Smart Citation

Collagen IV is present in the developing CNS during Drosophila neurogenesis

Mirre

Parco

Knibiehler

1992

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

By means of immunocytochemistry with a battery of specific antibodies, we describe the expression of the collagen IV chain produced by the gene DCg1 during the two phases of Drosophila neurogenesis. DgC1 was not expressed in neuronal tissues as shown by in situ hybridization, but the onset of its expression in cells of mesodermal origin was concomitant with the appearance of collagen IV on early axon pathways and peripheral nerve roots in the embryonic developing CNS. A similar situation was found during imaginal CNS development at metamorphosis, when collagen IV immunoreactivity was detected on centrifugal and centripetal nerve pathways, and specially on retinula axons that develop from the eye imaginal disc towards the lamina anlage in the brain optic lobe. Our results strongly suggest that collagen IV could be involved, together with other informative molecules of basement membranes, in a dynamic process of cell-matrix interactions during the establishment of initial axon pathways and neurite outgrowth in vivo.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Collagen IV Expression During the Formation Of The Imaginal Cnsmentioning

confidence: 99%

Collagen IV is present in the developing CNS during Drosophila neurogenesis

Mirre

Parco

Knibiehler

1992

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…As reported elsewhere [18,19], we have characterized three overlapping DNA sequences for the DCgl collagen gene [16] among numerous collagen-like genomic DNA clones from Drosophilu. Briefly, DCgl is a single-copy gene, located at the 2L-25C chromosomal locus; it is transcribed in a 6.4-kb mRNA and its expression is both differential and tissue-specific [ 191 during the course of development.…”

Section: Resultsmentioning

confidence: 85%

Evidence for a type-IV-related collagen in Drosophila melanogaster. Evolutionary constancy of the carboxyl-terminal noncollagenous domain

Cecchini¹,

Knibiehler²,

Mirre³

et al. 1987

Eur J Biochem

View full text Add to dashboard Cite

Type IV collagen, a major structural component of basement membrane, has been characterized only in vertebrates. It is unique among the collagenous proteins in that it forms specific lattice networks by end-to-end interactions. In particular, in mammals the C-terminal noncollagenous domain (NCl) of collagen IV was shown to be one of the major cross-linking sites in the network assembly. Here, we give the first direct evidence of type-IV-related collagen in invertebrates by sequence analysis of cDNA and genomic DNA clones for the 3'-end of a previously characterized Drosophilu collagen gene. The data describe the C-terminal 190 amino acid residues of the triple helix and the entire noncollagenous domain (231 amino acids) of the chain encoded for by this gene. Comparison with data reported for human and mouse al(1V) chains reveals that triple-helix regions are quite different, while NC1 structures are very similar. This suggests different constraints on triple-helix and NC1 domains during evolution. Present data support the assumption that the NC1 structure originated from duplication of an ancestral sequence; the extent of both interspecies and intramolecular homologies suggests the maintenance in vertebrates and invertebrates of an ancestral specific function.Type IV collagen, the major macromolecular component of basement membranes, is now well characterized in mammals [l -61. It consists of triple-helical thread-like particles, probably composed of two different types of CI chains (al, a2) [l-2, 4, 71. These particles differ markedly from interstitial ones in that they comprise, in addition to the central triple helix, a noncollagenous globular domain (NC1) at the C-terminal end, and a short triple-helix domain (7s domain) at the N-terminal which enable them to form, by end-to-end interactions, a large lattice network instead of cross-striated fibrils [2, 3, 6, 81. Similar properties have been reported for an intestinal basement membrane collagen of the helminth Ascuris suum [9] and for a putative basement membrane collagen obtained from Drosophilu KCo cell cultures [5]. These observations, along with data on mammalian systems, have suggested that the ability to form end-to-end interactions is a general property of basement membrane collagens [S, 91. However, information on the fine structure of these binding sites is at present restricted to nucleotide and amino acid sequences of the NC1 domain of the a1 type-IV chains of human [lo] and mouse [ll] origin.Therefore, because of the widespread occurrence of basement membranes throughout animal phyla, as well as their implication in cell and tissue interactions of importance for morphogenetic events [12 -151, the characterization of type IV collagen genes in invertebrates is of particular interest from the point of view of both developmental biology and evolution.A evidence that DCgl encodes a type IV collagen chain by nucleotide sequencing of the 3'-terminal coding region of complementary and genomic DNA clones. The polypeptide predicted from the nucleotide seq...

show abstract

“…Additionally, lamellocytes express an uncharacterized surface marker called L1 antigen (Asha et al, 2003). All Drosophila hemocytes specifically express the marker Hemese (He) (Kurucz et al, 2003), while a majority of plasmatocytes and crystal cells express the Collagens Viking and Cg25C (Le Parco et al, 1986;Yasothornsrikul et al, 1997) and the Von Willebrand-like factor Hemolectin (Hml) (Goto et al, 2003;Goto et al, 2001;Sinenko and Mathey-Prevot, 2004).…”

Section: Introductionmentioning

confidence: 99%

TheDrosophilalymph gland as a developmental model of hematopoiesis

et al. 2005

View full text Add to dashboard Cite

Drosophila hematopoiesis occurs in a specialized organ called the lymph gland. In this systematic analysis of lymph gland structure and gene expression, we define the developmental steps in the maturation of blood cells (hemocytes) from their precursors. In particular, distinct zones of hemocyte maturation, signaling and proliferation in the lymph gland during hematopoietic progression are described. Different stages of hemocyte development have been classified according to marker expression and placed within developmental niches: a medullary zone for quiescent prohemocytes, a cortical zone for maturing hemocytes and a zone called the posterior signaling center for specialized signaling hemocytes. This establishes a framework for the identification of Drosophila blood cells, at various stages of maturation, and provides a genetic basis for spatial and temporal events that govern hemocyte development. The cellular events identified in this analysis further establish Drosophila as a model system for hematopoiesis.Key words: Drosophila, Hematopoiesis, Blood, Hemocyte, Lymph gland SummaryThe Drosophila lymph gland as a developmental model of hematopoiesis

show abstract

Stage and tissue-specific expression of a collagen gene during Drosophila melanogaster development

Cited by 33 publications

References 18 publications

Collagen IV is present in the developing CNS during Drosophila neurogenesis

Collagen IV is present in the developing CNS during Drosophila neurogenesis

Evidence for a type-IV-related collagen in Drosophila melanogaster. Evolutionary constancy of the carboxyl-terminal noncollagenous domain

TheDrosophilalymph gland as a developmental model of hematopoiesis

Contact Info

Product

Resources

About