Staining Plant Cells with Silver. III. The Mechanism

Herickhoff, Lisa A.; Stack, Stephên M.; Robertson, J.C.

doi:10.3109/10520299209110030

Cited by 6 publications

(1 citation statement)

References 28 publications

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“…The NORs and nucleoli were identified by AgNO 3 staining as described by Howell and Black (1980) for L. odoratus, and Herickoff et al (1992) for the remaining species.…”

Section: Methodsmentioning

confidence: 99%

Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

1999

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Mitotic metaphase chromosomes and interphase nuclei of nine populations of three South American species of Lathyrus (L. pubescens, L. nervosus and L. crassipes) and six populations of the cultivated species L. odoratus were analyzed. All populations had 2n = 2x = 14 chromosomes. There were significant differences among populations within each species and among species in the number of metacentric, submetacentric and subtelocentric chromosomes, the number and location of secondary constrictions, chromosome length (longest and shortest), total haploid complement, arm ratio, and centromeric index. L. odoratus showed the highest tendency towards karyotype symmetry whereas the three South American species showed a moderate tendency towards asymmetry, with L. pubescens being the most asymmetrical. Silver staining was used to identify the nucleolar organizer regions (NORs) and the number of nucleoli per interphase nucleus in each species. In L. pubescens and L. nervosus, the NORs were located on the secondary constriction of the long arm of pair 7, in L. crassipes, the NOR was proximal being located in the pair of metacentric chromosomes, and in L. odoratus there were four terminal NORs on the short arms of pairs 4 and 5. The four species had a maximum of four nucleoli per interphase nucleus, indicating the presence of four regions with active ribosomal genes in each case.
Cromossomos em metáfases mitóticas e núcleos interfásicos em 9 populações de 3 espécies sul-americanas de Lathyrus (L. pubescens, L.nervosus e L.crassipes) e 6 populações da espécie cultivada L. odoratus foram analisados. Todas as populações apresentaram 2n = 2x = 14 cromossomos. As diferenças significativas observadas entre as populações dentro de cada espécie e entre as espécies foram: número de cromossomos metacêntricos, submetacêntricos e subtelocêntricos; número e localização das constrições secundárias; comprimento dos cromossomos (maior e menor); complemento total haplóide; razão braço longo/braço curto e índice centromérico. L. odoratus é a espécie com maior tendência simétrica em seu cariótipo, enquanto que nas três espécies sul-americanas os cariótipos têm tendência moderada para assimetria, sendo L. pubescens o mais assimétrico. Com nitrato de prata foi possível identificar as NORs e o número máximo de nucléolos por núcleo interfásico em cada espécie. Em L. pubescens e L. nervosus as NORs estão localizadas na constrição secundária do braço longo do par 7, em L. crassipes a NOR é proximal, localizada no par de cromossomos metacêntricos, e em L. odoratus foram observadas quatro NORs terminais nos braços curtos dos pares 4 e 5. As quatro espécies possuem número máximo de quatro nucléolos em cada núcleo interfásico, indicando quatro regiões com genes ribossomais ativos em cada espécie

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“…The NORs and nucleoli were identified by AgNO 3 staining as described by Howell and Black (1980) for L. odoratus, and Herickoff et al (1992) for the remaining species.…”

Section: Methodsmentioning

confidence: 99%

Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

1999

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Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization

Stack

Shearer

Lohmiller

et al. 2019

Methods in Molecular Biology

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Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading

Loidl

Lorenz

2009

Methods in Molecular Biology

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The fission yeast, Schizosaccharomyces pombe, much like the budding yeast, is a particularly well-suited model organism for genetic research. However, the miniscule size of both yeasts' nuclei has hindered their success as research models for cytologists. A solution to this problem is provided by the spreading of nuclei, which increases their volume and allows for a better spatial resolution of nuclear contents. Here we describe nuclear spreading in fission yeast. Spreading of meiotic nuclei is particularly helpful in exposing the linear elements (LinEs), which are the fission yeasts' rudimentary version of the synaptonemal complex. Although the LinEs' role is still not fully understood, they serve as important meiotic hallmarks and their presence and morphology can be used in characterizing meiotic mutants. We first describe methods to induce meiosis in liquid cell cultures, then outline a method to break down cell and nuclear membranes by detergent treatment to release chromatin on cytological slides, and finally provide a set of protocols for analyzing these nuclei by immunostaining and fluorescence in situ hybridization (FISH), and by electron microscopy.

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Staining Plant Cells with Silver. III. The Mechanism

Cited by 6 publications

References 28 publications

Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization

Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading

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