Staining Techniques and Biochemical Methods for the Identification of Fungi

Sangeetha, Jeyabalan; Thangadurai, Devarajan

doi:10.1007/978-1-4614-2356-0_19

Cited by 8 publications

(10 citation statements)

References 112 publications

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“…Examine the UV absorbance at 570 nm of each well. Using the formula below, calculate the antiadherence activity of the ZnO NPs and tetracycline [ 45 , 46 ].

…”

Section: Methodsmentioning

confidence: 99%

Trichoderma-Mediated ZnO Nanoparticles and Their Antibiofilm and Antibacterial Activities

Shobha

Ashwini

Ghazwani

et al. 2023

JoF

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Antimicrobial resistance is a major global health concern and one of the gravest challenges to humanity today. Antibiotic resistance has been acquired by certain bacterial strains. As a result, new antibacterial drugs are urgently required to combat resistant microorganisms. Species of Trichoderma are known to produce a wide range of enzymes and secondary metabolites that can be exploited for the synthesis of nanoparticles. In the present study, Trichoderma asperellum was isolated from rhizosphere soil and used for the biosynthesis of ZnO NPs. To examine the antibacterial activity of ZnO NPs against human pathogens, Escherichia coli and Staphylococcus aureus were used. The obtained antibacterial results show that the biosynthesized ZnO NPs were efficient antibacterial agents against the pathogens E. coli and S. aureus, with an inhibition zone of 3–9 mm. The ZnO NPs were also effective in the prevention of S. aureus biofilm formation and adherence. The current work shows that the MIC dosages of ZnO NPs (25, 50, and 75 μg/mL) have effective antibacterial activity and antibiofilm action against S. aureus. As a result, ZnO NPs can be used as a part of combination therapy for drug-resistant S. aureus infections, where biofilm development is critical for disease progression.

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“…Examine the UV absorbance at 570 nm of each well. Using the formula below, calculate the antiadherence activity of the ZnO NPs and tetracycline [ 45 , 46 ].

…”

Section: Methodsmentioning

confidence: 99%

Trichoderma-Mediated ZnO Nanoparticles and Their Antibiofilm and Antibacterial Activities

Shobha

Ashwini

Ghazwani

et al. 2023

JoF

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“…For example, the budding formation has been observed from yeast species, Z. kombuchaensis isolated from a dried tea fungus of Russian Kombucha [ 59 ]. Cellular morphological tests are usually obtained using the wet-mount method, by suspending the culture in saline and mixing it with dyes such as India ink, lactophenol cotton blue, calcofluor white or methylene blue staining [ 87 ].…”

Section: Phenotypic Characterisation and Identification Of Acetic Aci...mentioning

confidence: 99%

Kombucha: Production and Microbiological Research

Wang

Rutherfurd-Markwick

Zhang

et al. 2022

Foods

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Kombucha is a sparkling sugared tea commonly prepared using a sugared tea infusion and fermented at ambient temperature for several days using a cellulose pellicle also called tea fungus that is comprised of acetic acid bacteria and yeast. Consumption of Kombucha has been reported as early as 220 B.C. with various reported potential health benefits and appealing sensory properties. During Kombucha fermentation, sucrose is hydrolysed by yeast cells into fructose and glucose, which are then metabolised to ethanol. The ethanol is then oxidised by acetic acid bacteria (AAB) to produce acetic acid which is responsible for the reduction of the pH and also contributes to the sour taste of Kombucha. Characterisation of the AAB and yeast in the Kombucha starter culture can provide a better understanding of the fermentation process. This knowledge can potentially aid in the production of higher quality products as these microorganisms affect the production of metabolites such as organic acids which are associated with potential health benefits, as well as sensory properties. This review presents recent advances in the isolation, enumeration, biochemical characteristics, conventional phenotypic identification system, and modern genetic identification techniques of AAB and yeast present in Kombucha to gain a better understanding of the microbial diversity of the beverage.

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“…Caseinase: Fungal discs were aseptically placed on skimmed milk agar plates and incubated at room temperature for a period of 7 days. The presence of a distinct clear zone surrounding the fungal growth was indicative of positive caseinase production [66].…”

Section: Screening For Extracellular Enzymes Involved In Cypermethrin...mentioning

confidence: 99%

“…Fungal discs were aseptically placed on the plates, which were then incubated at room temperature for a period of 7 days. The development of a distinct clear zone around the fungal growth on the plates was regarded as a positive indication of cellulase production [66].…”

Section: Screening For Extracellular Enzymes Involved In Cypermethrin...mentioning

confidence: 99%

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Mycotransformation of Commercial Grade Cypermethrin Dispersion by Aspergillus terreus PDB-B Strain Isolated from Lake Sediments of Kulamangalam, Madurai

Kannan,

Baskaran,

Juliana Selvaraj

et al. 2024

Molecules

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A fungal isolate Aspergillus terreus PDB-B (accession number: MT774567.1), which could tolerate up to 500 mg/L of cypermethrin, was isolated from the lake sediments of Kulamangalam tropical lake, Madurai, and identified by internal transcribed spacer (ITS) sequencing followed by phylogenetic analysis. The biotransformation potential of the strain was compared with five other strains (A, J, UN2, M1 and SM108) as a consortium, which were tentatively identified as Aspergillus glaucus, Aspergillus niger, Aspergillus flavus, Aspergillus terreus, and Aspergillus flavus, respectively. Batch culture and soil microcosm studies were conducted to explore biotransformation using plate-based enzymatic screening and GC-MS. A mycotransformation pathway was predicted based on a comparative analysis of the transformation products (TPs) obtained. The cytotoxicity assay revealed that the presence of (3-methylphenyl) methanol and isopropyl ether could be relevant to the high rate of lethality.

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Staining Techniques and Biochemical Methods for the Identification of Fungi

Cited by 8 publications

References 112 publications

Trichoderma-Mediated ZnO Nanoparticles and Their Antibiofilm and Antibacterial Activities

Trichoderma-Mediated ZnO Nanoparticles and Their Antibiofilm and Antibacterial Activities

Kombucha: Production and Microbiological Research

Mycotransformation of Commercial Grade Cypermethrin Dispersion by Aspergillus terreus PDB-B Strain Isolated from Lake Sediments of Kulamangalam, Madurai

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