Standard and non standard products in combined T<sub>1</sub> plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors

Maden, B.E.H.; Forbes, J.

doi:10.1016/0014-5793(72)80733-6

Cited by 30 publications

(15 citation statements)

References 13 publications

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“…Even less is known about the conversion of uridines into pseudouridines in the course of the maturation process. Only limited data on the specificity and the timing of pseudouridylation of HeLa cell rRNA are available (12,13,22).…”

Section: Introductionmentioning

confidence: 99%

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Pseudouridylation of yeast ribosomal precursor RNA

et al. 1979

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The pseudouridylation of ribosomal RNA of Saccharomyces carlsbergensis was investigated with respect to its timing during the maturation of rRNA and its sequence specificity.Analysis of 37-S RNA, the common precursor to 17-S, 5.8-S and 26-S rRNA and most probably the primary ribosomal transcript, shows that this RNA molecule contains already most if not all of the 36-37 pseudouridine residues found in the mature rRNAs. Thus pseudouridylation is, like 2'-0-ribosemethylation, an early event in the maturation of rRNA, taking place immediately after, or even during, transcription.The data presented show that the non-conserved sequences of 37-S precursor rRNA contain very few pseudouridine residues if any. The pseudouridine residues within the rRNA sequences are apparently clustered to a certain degree as can be inferred from the occurrence of a single oligonucleotide containing 3 pseudouridines, which was obtained by digestion of 26-S rRNA with ribonuclease T1.

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Section: Introductionmentioning

confidence: 99%

“…(18). The isolation and purification of 29-S and 37-S precursor rRNA were performed as described previously (6 (13) and the digestion products were separated by two-dimensional electrophoresis (19). Labelled oligonucleotides were located by radioautography and numbered according to Klootwijk et al (14).…”

Section: Introductionmentioning

confidence: 99%

Pseudouridylation of yeast ribosomal precursor RNA

et al. 1979

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“…These techniques have been applied by us to the study of the poly(A) region of mouse globin mRNA (Mansbridge et al, 1973). Others have used a mixture of T1 plus pancreatic RNAases to analyse the methylated base sequences in rRNA (Maden & Forbes, 1972;) by using con-ventional 'fingerprinting' techniques (Brownlee, 1972). Similar digests of Rous sarcoma virus have been separated by homochromatography (Horst et al, 1972).…”

Section: Discussionmentioning

confidence: 99%

The fractionation of adenosine-rich oligoribonucleotides on polyethyleneimine-cellulose

Crossley

Mansbridge

Williamson

1974

Biochemical Journal

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After combined pancreatic and T(1) ribonuclease treatment of RNA, a characteristic series of products of the type A(n)N are obtained, where N can be any of the four ribonucleosides. Depending on whether phosphatase treatment is used before the addition of labelled phosphate at the 5'-terminus with bacteriophage phosphokinase, the labelled oligonucleotides obtained may or may not possess a phosphate group at the 3'- as well as the 5'-end. The behaviour of these characteristic products after electrophoresis on cellulose acetate strips followed by chromatography on polyethyleneimine-cellulose thin-layer plates was examined.

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“…Pseudouridylation occurs rapidly in the nucleolus upon transcription of rRNA (Granneman and Baserga 2004 ;Maden and Forbes 1972 ) , and studies indicate that global loss of rRNA pseudouridylation in yeast due to genetic deletion of Cbf5 results in pre-rRNA processing defects. Speci fi cally, Cbf5 depletion results in delayed production of 18S and 28S rRNA along with an accumulation of pre-rRNA species (Lafontaine et al 1998 ) .…”

Section: Role For Y Modi Fi Cations In Ribosome Biogenesismentioning

confidence: 99%

The “Fifth” RNA Nucleotide: A Role for Ribosomal RNA Pseudouridylation in Control of Gene Expression at the Translational Level

McMahon

Bellodi

Ruggero

2012

Biophysical Approaches to Translational Control of Gene Expression

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Standard and non standard products in combined T₁ plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors

Cited by 30 publications

References 13 publications

Pseudouridylation of yeast ribosomal precursor RNA

Pseudouridylation of yeast ribosomal precursor RNA

The fractionation of adenosine-rich oligoribonucleotides on polyethyleneimine-cellulose

The “Fifth” RNA Nucleotide: A Role for Ribosomal RNA Pseudouridylation in Control of Gene Expression at the Translational Level

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Standard and non standard products in combined T1 plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors

Cited by 30 publications

References 13 publications

Pseudouridylation of yeast ribosomal precursor RNA

Pseudouridylation of yeast ribosomal precursor RNA

The fractionation of adenosine-rich oligoribonucleotides on polyethyleneimine-cellulose

The “Fifth” RNA Nucleotide: A Role for Ribosomal RNA Pseudouridylation in Control of Gene Expression at the Translational Level

Contact Info

Product

Resources

About

Standard and non standard products in combined T₁ plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors