Standard Cremaster Muscle Model for Ischemia Reperfusion

Molski, Michal; Yazici, Ilker; Siemionow, Maria

doi:10.1007/978-1-4471-6335-0_9

Cited by 1 publication

(1 citation statement)

References 19 publications

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“…This well-standardized surgical procedure can be a useful tool for visualizing and analyzing capillary perfusion, leukocyte–endothelial interaction, microvascular response to different stimuli and endothelial permeability in a defined environment ( Donndorf et al, 2013 ; Molski et al, 2015 ). It can also be used to study blood cell interactions under influence of different drugs and chemokines ( Reichel et al, 2012 ; Rius and Sanz, 2015 ) as well as ischemia-reperfusion (IR) injury and local effects of systemic conditions ( Molski et al, 2015 ). Cremaster muscle is an acute IVM model.…”

Section: Models/operation Techniquesmentioning

confidence: 99%

Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering

Vaghela

Arkudas

Horch

et al. 2021

Front. Bioeng. Biotechnol.

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Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs.

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