Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Parasuraman, Paramanantham; Busi, Siddhardha; Lee, Jung-Kul

doi:10.1007/978-981-99-8799-3_4

ESKAPE Pathogens 2024

DOI: 10.1007/978-981-99-8799-3_4

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Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Paramanantham Parasuraman,

Siddhardha Busi,

Jung-Kul Lee

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2024

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Molecular characterization of virulence genes in quorum sensing system of different pathogenic strains of Pseudomonas aeruginosa from neonates with pulmonary infections using PCR as efficient technique

Chaudhry,

Rehman,

Zahid

et al. 2024

Preprint

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Background: The expectation from the current research study was to genetically identify the virulence genes involved in quorum sensing (QS) in different strains of Pseudomonas aeruginosa, as the QS controls production of many virulence factors. Objective: The intention of current research study was to isolate the pathogenic strains of P. aeruginosa from sputum samples of neonates and infants and their molecular characterization. Methods: P. aeruginosawere obtained from Microbiology Laboratory, Department of Zoology, Government College University Lahore were grown on selective media. Biochemical and molecular characterization was done. Molecular characterization was done by specific primers of quorum sensing virulent genes. Amplified genes were sequenced, and accession numbers were obtained from the NCBI site. Genes lasR, lasI, rhlR, and rhlI were identified in P. aeruginosa strains (ss5, ss6, and ss11). Results: Current study revealed that these virulence genes are the main contributors of resistance of P. aeruginosa strains against different antibacterial agents. Conclusion: P. aeruginosa is a Gram-negative bacterium with diverse metabolic capacity to regulate survival under many different conditions and can be highly resistant to antibiotics, facilitating its spread in diverse habitats, particularly in hospitals. These virulent genes contribute to the overall pathogenic potential of P. aeruginosa that facilitates its ability to cause disease.

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Molecular characterization of virulence genes in quorum sensing system of different pathogenic strains of Pseudomonas aeruginosa from neonates with pulmonary infections using PCR as efficient technique

Chaudhry,

Rehman,

Zahid

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Standard Microbiological Techniques (Staining, Morphological and Cultural Characteristics, Biochemical Properties, and Serotyping) in the Detection of ESKAPE Pathogens

Cited by 1 publication

References 183 publications

Molecular characterization of virulence genes in quorum sensing system of different pathogenic strains of Pseudomonas aeruginosa from neonates with pulmonary infections using PCR as efficient technique

Molecular characterization of virulence genes in quorum sensing system of different pathogenic strains of Pseudomonas aeruginosa from neonates with pulmonary infections using PCR as efficient technique

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