Standard Reference Materials

Cohen, Alejandro M.; Hertz, Harry S.; Mandel, John; Schaffer, Robert

doi:10.6028/nbs.sp.260-80

Cited by 5 publications

(7 citation statements)

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“…Then, 50 μL of the resulting solution were evaporated again, and the formed precipitate was treated with 50 μL of N,O-bis(trimethylsilyl)acetamide at room temperature for 0.5 h. The solution (3-μL portion) was injected into a gas chromatograph equipped with a mass spectrometric detector that recorded ions with mass-to-charge ratios (m/z) of 458 and 465 of chemical compounds separated in the analytical column; the total cholesterol concentration in serum was then calculated. The mean-square deviation of the relative error of determinations of total cholesterol concentrations of 3.4298, 4.719, 6.1494, 7.4556, and 8.7881 mM in serum corresponded to 0.37, 0.35, 0.38, 0.34, and 0.36% [8].…”

Section: Research In the 1980smentioning

confidence: 92%

Determination of cholesterol in blood. Part 2

Buzanovskii¹

2016

Ref. J. Chem.

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The chronological development of procedures for determining the cholesterol concentration of in plasma, serum, and whole blood is presented in the review. It is stated that, since the correlation between the risk of development of cardiovascular diseases and the concentrations of total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in human blood was established by numerous medical studies, procedures for the measurement of these parameters have been developed most actively. A brief overview of these procedures and the results of their comparative testing in patient medical examinations are given. Classifications are proposed for procedures for determining total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in the blood. The mechanism of action of the chemical reactions taking place in these procedures, advantages and disadvantages, and priorities for their application are considered. Promising directions for the development and improvement of procedures to ensure more accurate measurements of the blood cholesterol concentration, are mentioned, and alternative means of determining the risk of cardiovascular disease are discussed.

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Section: Research In the 1980smentioning

confidence: 92%

Determination of cholesterol in blood. Part 2

Buzanovskii¹

2016

Ref. J. Chem.

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“…Stable isotope dilution mass spectrometry (ID-MS) incorporates a precise method that has proved successful for the measurement of small molecules, including cholesterol [1], cortisol [2], and dioxin [3]. However, its use for macromolecules such as proteins is less developed due to the difficulty in ionization.…”

Section: Introductionmentioning

confidence: 99%

Development of an isotope dilution mass spectrometry assay for HbA1c based on enzyme-cleaved peptide analysis

Iguchi

Nakanishi

Miyazaki

et al. 2004

Journal of Chromatography B

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“…The LC-ESI-MS method was devised by Kobold et al [4]. Isotope dilution MS (IDMS) has been widely accepted as a primary method with high precision, and it has proved successful for the measurement of small molecules [5][6][7][8]. In recent years, it has been successfully expanded for absolute protein quantitation, and several proteins have been quantified by the IDMS method [9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Development of hemoglobin A1c certified reference material by liquid chromatography isotope dilution mass spectrometry

Yang

et al. 2012

Anal Bioanal Chem

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We report the development of a National Institute of Metrology (NIM) hemoglobin A(1c) (HbA(1c)) certified reference material (CRM). Each CRM unit contains about 10 μL of hemoglobin. Both hemoglobin and glycated hemoglobin were quantitatively determined by high-performance liquid chromatography (HPLC)-isotope dilution mass spectrometry (IDMS) with synthesized VHLTPE and glycated VHLTPE as standards. The mass fraction of synthesized VHLTPE or glycated VHLTPE was also quantitatively determined by HPLC-IDMS with NIM amino acid CRMs as standards. The homogeneity and stability of the CRMs were examined with a commercial HbA(1c) analyzer based on the HPLC principle. Fifteen units were randomly selected for homogeneity examination, and statistical analysis showed there was no inhomogeneity. Examination of the stability showed that the CRM was stable for at least 6 months at -80 °C. Uncertainty components of the balance, amino acid purity, hydrolysis and proteolysis efficiency, method reproducibility, homogeneity, and stability were taken into consideration for uncertainty evaluation. The certified value of NIM HbA(1c) CRM was expressed as the ratio of HbA(1c) to total hemoglobin in moles, and was (9.6 ± 1.9)%. The CRM can be used as a calibration or validation standard for clinical diagnostics. It is expected to improve the comparability for HbA(1c) measurement in China.

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Standard Reference Materials

Cited by 5 publications

References 0 publications

Determination of cholesterol in blood. Part 2

Determination of cholesterol in blood. Part 2

Development of an isotope dilution mass spectrometry assay for HbA1c based on enzyme-cleaved peptide analysis

Development of hemoglobin A1c certified reference material by liquid chromatography isotope dilution mass spectrometry

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