Standardization and validation of a high-performance thin-layer chromatography method for the quantification of aflatoxin B1 and its application in surveillance of contamination level in marketed food commodities from the Mumbai region

Pradhan, Santosh; Ananthanarayan, Laxmi

doi:10.1007/s00764-020-00073-6

Cited by 15 publications

(5 citation statements)

References 38 publications

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“…The acid modified QuEChERS method was more effective than the standard QuEChERS method for removing matrix co‐extractants. A modified QuEChERS method using ACN‐methanol (2:3) as extraction solvent was used for the determination of aflatoxin B1 in food commodities (groundnut, corn, rice, wheat, and dried chilies) by HPTLC [60]. The modified QuEChERS method was the most efficient of several methods evaluated including UASE and LLE.…”

Section: Solvent Extraction (Solids)mentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Section: Solvent Extraction (Solids)mentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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“…Enzyme-linked immunosorbent assay (ELISA), highperformance liquid chromatography (HPLC) and thin-layer chromatography are the traditional quantitative techniques to detect AFB1. [3][4][5] However, these methods are expensive, timeconsuming, or require sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

A novel strategy for analyzing aptamer dominated sites and detecting AFB1 based on CRISPR–Cas12a

Xing

Niu

2023

Sens. Diagn.

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“…Thin-layer chromatography (TLC) with LOD of 1.133 ng/band (Pradhan and Ananthanarayan, 2020;Salisu et al, 2021) and highperformance liquid chromatography (HPLC) with LOD of 0.012 ng/mL (Saini and Abdel-Rehim, 2020;Shuib and Saad, 2022) are commonly reported techniques for the separation of aflatoxins from other secondary metabolites and their subsequent analysis. On the other side, immunochemical methods exhibited LOD values down to 0.12 pg/mL and include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and immunoaffinity column assay (ICA), which take advantage of the affinity of monoclonal or polyclonal antibodies for the identification and quantification of aflatoxins in agricultural products (Gross et al, 2019;Raysyan et al, 2020;Zhan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Ensuring food safety with molecularly imprinted polymers: innovative methods for the detection of aflatoxins in food and feed samples

Ali,

Sadiqa,

Ilyas

et al. 2023

Front. Sustain. Food Syst.

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Aflatoxins, a group of mycotoxins, represent a heterogeneous class of secondary metabolites that pose a significant risk to food safety and public health due to their potent toxicity. Aflatoxins are widely distributed in the environment, with high levels frequently observed in hot and humid conditions. There is an ongoing development of various methods for detecting aflatoxins in food and feed samples. Herein, a review of these methods is presented with special emphasis on molecularly imprinted polymers (MIPs) as selective materials for aflatoxins’ detection. The key findings of various methods for real-time analysis of food and feed samples are presented and analyzed, providing a comparative assessment of their performance. Furthermore, the challenges and limitations of these methods are discussed, considering their commercialization prospects and real-world requirements.

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Standardization and validation of a high-performance thin-layer chromatography method for the quantification of aflatoxin B1 and its application in surveillance of contamination level in marketed food commodities from the Mumbai region

Cited by 15 publications

References 38 publications

Sample preparation for planar chromatography

Sample preparation for planar chromatography

A novel strategy for analyzing aptamer dominated sites and detecting AFB1 based on CRISPR–Cas12a

Ensuring food safety with molecularly imprinted polymers: innovative methods for the detection of aflatoxins in food and feed samples

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